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Adenosylmethionine Metabolism in Human Inflammation

6 августа 2015 г. обновлено: En-Pei Isabel Chiang, National Chung Hsing University

Translational Study on the Regulation of Adenosylmethionine Synthesis During Chronic Inflammation

The investigators propose to conduct a translational study on the regulation of S-adenosylmethionine synthesis and cellular methylation reactions during chronic inflammation. Development of in vitro cell models may reveal the regulatory mechanisms by which specific inflammatory mediators cause metabolic changes and alter DNA methylation status. Metabolic and pharmacological studies in the in vivo models will enable us to better understand the regulation of inter-organ homeostasis of S-adenosyl methionine and help identify tissue specific biomarkers for methylation and epigenetic modifications in different stage of chronic inflammation. The clinical study in human subjects will help distinguish the impacts of autoimmune rheumatic disease, degenerated joint disease, or specific medication use on significant clinical and biochemical markers in folate and vitamin B6 metabolic pathways.The Investigators hope the present study can identify specific clinical markers for potential epigenetic changes in patients suffering from chronic inflammation, which will contribute to better clinical management of these diseases in humans.

Обзор исследования

Статус

Неизвестный

Условия

Подробное описание

The significance of epigenetic alterations in autoimmune rheumatic diseases and degenerated joint diseases has drawn great attention among clinicians and researchers. Aberrant methylation status has been demonstrated in human chronic inflammation yet more efforts have focused on global and sequence-specific hypomethylation and overexpression of specific genes. Few studies investigated the regulation of S-adenosylmethionine homeostasis and regulation during inflammation. At present the relevance and regulation of the complex epigenetic profiles and their modifications among different tissues and organs during inflammation remain largely unknown.

Тип исследования

Наблюдательный

Регистрация (Ожидаемый)

250

Критерии участия

Исследователи ищут людей, которые соответствуют определенному описанию, называемому критериям приемлемости. Некоторыми примерами этих критериев являются общее состояние здоровья человека или предшествующее лечение.

Критерии приемлемости

Возраст, подходящий для обучения

От 18 лет до 80 лет (Взрослый, Пожилой взрослый)

Принимает здоровых добровольцев

Нет

Полы, имеющие право на обучение

Все

Метод выборки

Вероятностная выборка

Исследуемая популяция

patients with arthritis or healthy adults

Описание

Inclusion Criteria:

> 18 years

Exclusion Criteria:

pregnancy,
anemia (hemoglobin 10 mg/dL or lower),
thrombocytopenia (platelet count below 50,000 cells/μL),
abnormal serum hepatic transaminase (aspartate aminotransferase or alanine aminotransferase above 50 IU/L),
diabetes or cancer

Учебный план

В этом разделе представлена подробная информация о плане исследования, в том числе о том, как планируется исследование и что оно измеряет.

Как устроено исследование?

Количество групп / когорт

Когорты и вмешательства

Группа / когорта
Arthritis subjects with arthritis
Health control subjects Health control

Что измеряет исследование?

Первичные показатели результатов

Мера результата	Мера Описание	Временное ограничение
s-adenosylmethionine Временное ограничение: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
homocysteine Временное ограничение: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
folate Временное ограничение: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
vitamin B6 Временное ограничение: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor

Вторичные показатели результатов

Мера результата	Мера Описание	Временное ограничение
blood amino acid profile (serine, glycine, methionine,cysteine, cystathionine, dimethylglycine) Временное ограничение: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
gene expression of target enzymes in PBMCs Временное ограничение: Blood were collected at admission.Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses	Blood were collected at admission.Blood were dawn again 1mo later if his medication was changed by the doctor
enzyme activities of S-adenosylmethionine synthase in RBC Временное ограничение: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
vitamin B6 metabolic enzyme in RBC Временное ограничение: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
polymorphisms in one carbon metabolism enzymes in PBMCs Временное ограничение: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor

Соавторы и исследователи

Здесь вы найдете людей и организации, участвующие в этом исследовании.

Спонсор

National Chung Hsing University

Соавторы

National Science Council, Taiwan

Ministry of Science and Technology, Taiwan

Следователи

Главный следователь: En-Pei I Chiang, PhD, Department of Food Science and Biotechnology, National Chung Hsing University

Даты записи исследования

Эти даты отслеживают ход отправки отчетов об исследованиях и сводных результатов на сайт ClinicalTrials.gov. Записи исследований и сообщаемые результаты проверяются Национальной медицинской библиотекой (NLM), чтобы убедиться, что они соответствуют определенным стандартам контроля качества, прежде чем публиковать их на общедоступном веб-сайте.

Изучение основных дат

Начало исследования

1 января 2011 г.

Первичное завершение (Действительный)

1 июля 2014 г.

Завершение исследования (Ожидаемый)

1 августа 2015 г.

Даты регистрации исследования

Первый отправленный

28 июля 2015 г.

Впервые представлено, что соответствует критериям контроля качества

6 августа 2015 г.

Первый опубликованный (Оценивать)

11 августа 2015 г.

Обновления учебных записей

Последнее опубликованное обновление (Оценивать)