Anxiety and Depression in IBS: A Brain-Gut Axis Study

Inclusion Criteria:

1. Right-handed, aged 18-60 years, with more than 6 years of education;
2. Meeting the Rome IV diagnostic criteria for IBS: Symptoms have been present for at least 6 months, and for the last 3 months, the following criteria are met: recurrent abdominal pain or discomfort, with symptoms occurring on at least 3 days per month in the last 3 months, associated with two or more of the following: 1) Improvement with defecation; 2) Onset associated with a change in frequency of stool; 3) Onset associated with a change in form (appearance) of stool;
3. The following symptoms support the diagnosis of IBS: 1) Abnormal stool frequency: <3 bowel movements per week or >3 bowel movements per day; 2) Abnormal stool form: lumpy/hard stool or mushy/watery stool; 3) Straining during defecation; 4) Urgency or a feeling of incomplete evacuation; 5) Passage of mucus; 6) Bloating.

Exclusion Criteria:

1. History of anti-anxiety/depressant medication use (e.g., SSRI/SNRI, Chinese patent medicines) within the past 1 month;
2. History of antibiotic or probiotic use within the past 1 month;
3. Other gastrointestinal diseases such as IBD, intestinal obstruction, or history of gastrointestinal surgery;
4. Current or past history of psychiatric or neurological disorders, such as psychosis, brain tumors, or impaired consciousness;
5. Concurrent severe hepatic or renal insufficiency, cardiovascular or cerebrovascular diseases, or malignant tumors; 7.0T MRI Contraindications: Presence of ferromagnetic implants (e.g., cardiac pacemakers, defibrillators, neurostimulators, aneurysm clips, cochlear implants, or any metallic foreign bodies); Non-ferromagnetic implants (e.g., titanium alloy, orthopedic implants), various types of intrauterine devices, various types of non-removable dentures (including dental implants); Presence of metallic foreign bodies (e.g., metal fragments, shrapnel, or filings in the eyes or body), such as in individuals engaged in welding work or with a history of metal item trauma; Tattoos or permanent makeup (e.g., eyebrows, lips) applied within the past month; Claustrophobia; Fever.

HC Group Inclusion Criteria (all must be met):

1. Right-handed, aged 18-60 years, with more than 6 years of education;
2. All physiological indicators are within normal ranges, with no signs of disease;
3. No family history of psychiatric or neurological disorders.