Proteomic Study of Urinary Stone Disease
Urinary Proteomic Profiling Using ProteinChip SELDI-TOF-MS: A Potential Means of Identifying Protein Biomarkers of Urinary Stone Formers
調査の概要
詳細な説明
Urinary stone disease affects 10% of the Canadian population during their lifetime and approximately half of these patients will have another episode within ten years. Currently, patients undergo metabolic testing (serum and 24 hour urine tests) to identify modifiable risk factors; however, no modifiable risk factors are identified in many patients, yet they continue to form stones. New techniques must be developed to identify stone patients at risk for future recurrences and ultimately to develop more specific prevention strategies.
Urinary protein levels are not routinely measured in stone patients while there is strong evidence that proteins play a role in the etiology of stones. The purpose of this study is to examine the urinary and serum proteins of stone formers compared to healthy subjects utilizing the high throughput method, Surface Enhanced Laser Desorption/Ionization (SELDI). We hypothesize that there is a unique set of proteins expressed in serum and urine in stone patients that can be detected by SELDI. Once a protein is identified as a biomarker, a specific assay similar to a quick and affordable dipstick test may be developed to identify those stone patients at risk of future stones. Ultimately, this will better our understanding of stone disease and help develop new prevention strategies.
Comparisons: protein profiles (serum/urine) of stone patients both during the presence of a stone and 6 weeks after they have passed it. comparison of stone profiles of stone patients with controls (non-forming stone patients).
研究の種類
入学 (実際)
連絡先と場所
研究場所
-
-
Ontario
-
London、Ontario、カナダ、N6A 4V2
- St. Joseph's Health Care London
-
-
参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
サンプリング方法
調査対象母集団
説明
Inclusion Criteria:
Controls:
- Ages 18 to 65 years of age
- No history of stone disease and no radiographical evidence of stone (as demonstrated by negative ultrasound)
- No family history of stones
- Healthy and no autoimmune or systemic disease that may affect renal function (see exclusion criteria)
Stone patients
- Ages 18 to 65 years of age
- Solitary stone of any size, in any location along the urinary tract (except lower renal calyceal stones and bladder stones)
- Radiology of any modality proving the existence of the stone (ultrasound, computed tomography, intravenous pyelogram, kidney-ureter-bladder x-ray)
Exclusion Criteria:
ALL:
- Pregnant females
- Male patients treated for with benign prostate hyperplasia (BPH) (ongoing medical treatment or surgical intervention within 6 months)
- Positive urine culture
- Any cancer (excluding superficial skin, brain)
- Chronic Recurrent urinary infections (prostate, cystitis, vaginosis/vaginitis)
- Gross hematuria
- Autoimmune disease that may affect renal function (eg Systemic lupus erythematosus)
- Renal dysfunction or its common causes:
- Diabetes
- Uncontrolled hypertension (with concurrent microalbuminuria) (diastolic BP > 90 mmHg)
- glomerulonephritis
- Renal transplant
- Genetic stone disease (e.g. Cystine stones, xanthinuria)
- Medullary sponge kidney, or other renal anomalies such as horseshoe kidney
- GI disorders: Inflammatory bowel disease, short bowel
- Hypercalcemic disorders (hyperparathyroidism, sarcoidosis, Paget's disease)
- Renal tubular acidosis
- Immunodeficient patients e.g. HIV (indinavir stones)
- Unable to provide informed consent
- Anyone in the opinion of the investigator who would be inappropriate
Controls :
- In addition to criteria above.....
- persistent thiazide use
- Family history of stones (this will exclude any genetic factors since a positive family history increases the risk of urolithiasis)
研究計画
研究はどのように設計されていますか？
デザインの詳細
協力者と研究者
捜査官
- 主任研究者：John D Denstedt, MD, FRCSC、The University of Western Ontario (Professor)
研究記録日
主要日程の研究
研究開始
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (見積もり)
学習記録の更新
投稿された最後の更新 (見積もり)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
この情報は、Web サイト clinicaltrials.gov から変更なしで直接取得したものです。研究の詳細を変更、削除、または更新するリクエストがある場合は、register@clinicaltrials.gov。 までご連絡ください。 clinicaltrials.gov に変更が加えられるとすぐに、ウェブサイトでも自動的に更新されます。