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Proteomic Study of Urinary Stone Disease

2. september 2009 oppdatert av: Lawson Health Research Institute

Urinary Proteomic Profiling Using ProteinChip SELDI-TOF-MS: A Potential Means of Identifying Protein Biomarkers of Urinary Stone Formers

Urinary protein levels are not routinely measured in stone patients while there is strong evidence that proteins play a role in the etiology of stones. The purpose of this study is to examine the urinary and serum proteins of stone formers compared to healthy subjects utilizing the high throughput method, Surface Enhanced Laser Desorption/Ionization (SELDI). We hypothesize that there is a unique set of proteins expressed in serum and urine in stone patients that can be detected by SELDI. Ultimately, this will better our understanding of stone disease and help develop new prevention strategies.

Studieoversikt

Status

Fullført

Forhold

Detaljert beskrivelse

Urinary stone disease affects 10% of the Canadian population during their lifetime and approximately half of these patients will have another episode within ten years. Currently, patients undergo metabolic testing (serum and 24 hour urine tests) to identify modifiable risk factors; however, no modifiable risk factors are identified in many patients, yet they continue to form stones. New techniques must be developed to identify stone patients at risk for future recurrences and ultimately to develop more specific prevention strategies.

Urinary protein levels are not routinely measured in stone patients while there is strong evidence that proteins play a role in the etiology of stones. The purpose of this study is to examine the urinary and serum proteins of stone formers compared to healthy subjects utilizing the high throughput method, Surface Enhanced Laser Desorption/Ionization (SELDI). We hypothesize that there is a unique set of proteins expressed in serum and urine in stone patients that can be detected by SELDI. Once a protein is identified as a biomarker, a specific assay similar to a quick and affordable dipstick test may be developed to identify those stone patients at risk of future stones. Ultimately, this will better our understanding of stone disease and help develop new prevention strategies.

Comparisons: protein profiles (serum/urine) of stone patients both during the presence of a stone and 6 weeks after they have passed it. comparison of stone profiles of stone patients with controls (non-forming stone patients).

Studietype

Observasjonsmessig

Registrering (Faktiske)

20

Kontakter og plasseringer

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Studiesteder

  • Canada
    • Ontario
      • London, Ontario, Canada, N6A 4V2
        • St. Joseph's Health Care London

Deltakelseskriterier

Forskere ser etter personer som passer til en bestemt beskrivelse, kalt kvalifikasjonskriterier. Noen eksempler på disse kriteriene er en persons generelle helsetilstand eller tidligere behandlinger.

Kvalifikasjonskriterier

Alder som er kvalifisert for studier

18 år til 65 år (Voksen, Eldre voksen)

Tar imot friske frivillige

Ja

Kjønn som er kvalifisert for studier

Alle

Prøvetakingsmetode

Ikke-sannsynlighetsprøve

Studiepopulasjon

This observational study will compare stone formers meeting the inclusion criteria with a cohort of age and sex matched non-stone formers as controls.

Beskrivelse

Inclusion Criteria:

  • Controls:

    • Ages 18 to 65 years of age
    • No history of stone disease and no radiographical evidence of stone (as demonstrated by negative ultrasound)
    • No family history of stones
    • Healthy and no autoimmune or systemic disease that may affect renal function (see exclusion criteria)

Stone patients

  • Ages 18 to 65 years of age
  • Solitary stone of any size, in any location along the urinary tract (except lower renal calyceal stones and bladder stones)
  • Radiology of any modality proving the existence of the stone (ultrasound, computed tomography, intravenous pyelogram, kidney-ureter-bladder x-ray)

Exclusion Criteria:

  • ALL:

    • Pregnant females
    • Male patients treated for with benign prostate hyperplasia (BPH) (ongoing medical treatment or surgical intervention within 6 months)
    • Positive urine culture
    • Any cancer (excluding superficial skin, brain)
    • Chronic Recurrent urinary infections (prostate, cystitis, vaginosis/vaginitis)
    • Gross hematuria
    • Autoimmune disease that may affect renal function (eg Systemic lupus erythematosus)
    • Renal dysfunction or its common causes:
    • Diabetes
    • Uncontrolled hypertension (with concurrent microalbuminuria) (diastolic BP > 90 mmHg)
    • glomerulonephritis
    • Renal transplant
    • Genetic stone disease (e.g. Cystine stones, xanthinuria)
    • Medullary sponge kidney, or other renal anomalies such as horseshoe kidney
    • GI disorders: Inflammatory bowel disease, short bowel
    • Hypercalcemic disorders (hyperparathyroidism, sarcoidosis, Paget's disease)
    • Renal tubular acidosis
    • Immunodeficient patients e.g. HIV (indinavir stones)
    • Unable to provide informed consent
    • Anyone in the opinion of the investigator who would be inappropriate

Controls :

  • In addition to criteria above.....
  • persistent thiazide use
  • Family history of stones (this will exclude any genetic factors since a positive family history increases the risk of urolithiasis)

Studieplan

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Hvordan er studiet utformet?

Designdetaljer

Samarbeidspartnere og etterforskere

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Sponsor

Lawson Health Research Institute

Samarbeidspartnere

University of Western Ontario, Canada
The Physicians' Services Incorporated Foundation

Etterforskere

  • Hovedetterforsker: John D Denstedt, MD, FRCSC, The University of Western Ontario (Professor)

Studierekorddatoer

Disse datoene sporer fremdriften for innsending av studieposter og sammendragsresultater til ClinicalTrials.gov. Studieposter og rapporterte resultater gjennomgås av National Library of Medicine (NLM) for å sikre at de oppfyller spesifikke kvalitetskontrollstandarder før de legges ut på det offentlige nettstedet.

Studer hoveddatoer

Studiestart

1. januar 2005

Primær fullføring (Faktiske)

1. november 2008

Studiet fullført (Faktiske)

1. november 2008

Datoer for studieregistrering

Først innsendt

12. september 2005

Først innsendt som oppfylte QC-kriteriene

12. september 2005

Først lagt ut (Anslag)

20. september 2005

Oppdateringer av studieposter

Sist oppdatering lagt ut (Anslag)

3. september 2009

Siste oppdatering sendt inn som oppfylte QC-kriteriene

2. september 2009

Sist bekreftet

1. september 2009

Mer informasjon

Begreper knyttet til denne studien

Nøkkelord

Ytterligere relevante MeSH-vilkår

Andre studie-ID-numre

  • R-04-481
  • PSI 04-041

Denne informasjonen ble hentet direkte fra nettstedet clinicaltrials.gov uten noen endringer. Hvis du har noen forespørsler om å endre, fjerne eller oppdatere studiedetaljene dine, vennligst kontakt register@clinicaltrials.gov. Så snart en endring er implementert på clinicaltrials.gov, vil denne også bli oppdatert automatisk på nettstedet vårt. .

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