The Impact of Cigarette Smoking on Periodontal Therapy
Outcomes of Periodontal Therapy in Smokers and Non-smokers With Chronic Periodontitis
調査の概要
状態
詳細な説明
The overall aim of this work was to study clinical outcomes of active and supportive periodontal therapy in smokers and non-smokers with chronic periodontitis at patient, tooth, and site level. Moreover, to compare the periopathogenic microflora and inflammatory markers in gingival crevicular fluid and in blood in smokers and non-smokers following therapy.
Eighty patients, 40 smokers and 40 non-smokers, with moderate to severe chronic periodontitis were included in this prospective cohort study and treated non-surgically and surgically, and then followed-up in a supportive periodontal therapy program for 12 months. Smoking status was validated measuring serum cotinine levels at pre-treatment and 12 months following supportive periodontal therapy.
Clinical measurements included full mouth recordings of clinical attachment level, probing depth, bleeding on probing, and plaque index at pre-treatment and following active and supportive periodontal therapy. At the same timepoints, subgingival plaque samples of 20 subgingival periopathogenic bacterial species were analysed using checkerboard DNA-DNA hybridization. Blood samples (PAXgeneBlood), serum, gingval crevicular fluid were also collected at the three timepoints.
研究の種類
入学 (実際)
段階
- 適用できない
参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
説明
Inclusion Criteria:
- healthy subjects
- age 35-75 years
- diagnosed with chronic periodontitis
- at least four non-adjacent teeth with proximal sites with a PD ≥6 mm and clinical attachment loss ≥5 mm with BoP and no radiographic signs of apical pathology.
- either smokers (>10 cigarettes per day for at least 5 years) or non-smokers (never smoked or not within the last 5 years).
Exclusion Criteria:
- subjects who presented with any current medical condition or used medications known to affect periodontal healing
- incorrectly reported smoking status
- use of antibiotics within 6 months of the study
- received subgingival scaling within 6 months of the study
研究計画
研究はどのように設計されていますか？
デザインの詳細
- 主な目的：処理
- 割り当て：非ランダム化
- 介入モデル：並列代入
- マスキング：なし（オープンラベル）
武器と介入
参加者グループ / アーム |
介入・治療 |
|---|---|
|
アクティブコンパレータ：Cigarette smokers with periodontitis
Periodontal therapy in cigarette smokers
|
Non-surgical and surgical periodontal therapy in cigarette smokers and non-smokers
他の名前：
|
|
アクティブコンパレータ：Non-smokers with periodontitis
Periodontal therapy in non-smokers
|
Non-surgical and surgical periodontal therapy in cigarette smokers and non-smokers
他の名前：
|
この研究は何を測定していますか？
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
|
PD ≥5 mm with BoP
時間枠：24 months
|
Corrected for clustering within patients, teeth, and sites over time, was the unit of the analysis and PD ≥5 mm with BoP was the outcome (dependent variable) dichotomized as present (1) or absent (0).
|
24 months
|
二次結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
|
Teeth present
時間枠：24 months
|
Number of teeth
|
24 months
|
|
Clinical periodontal attachmentloss
時間枠：24 months
|
Clinical attachment loss in mm
|
24 months
|
|
Periodontal pocket depth
時間枠：24 months
|
Probing depth in mm
|
24 months
|
|
Bleeding at site level
時間枠：24 months
|
Bleeding on probing (yes or no)
|
24 months
|
|
Bleeding at patient level
時間枠：24 months
|
Bleeding index in percentage (%)
|
24 months
|
|
Plaque at patient level
時間枠：24 months
|
Plaque index in percentage (%)
|
24 months
|
|
Amount of periopathogenic bacteria
時間枠：24 months
|
Subgingival plaque samples of 20 subgingival periopathogenic bacterial species were analysed using checkerboard DNA-DNA hybridization.
|
24 months
|
|
Inflammatory gingival crevicular fluid markers (proteins)
時間枠：24 months
|
Concentrations of gingival crevicular fluid biomarkers measured in pg/mLwere analysed using multiplex and singleplex micro-bed immunoassays.
|
24 months
|
|
Serum proteins
時間枠：24 months
|
Concentration of inflammatory serum markers measured in pg/ɥL determined using multiplex kit Bio-Plex Human ProTM Assay.
|
24 months
|
|
Gene expression in blood
時間枠：24 months
|
Inflammatory gene (RNA) expression levels is estimated by counting the reads that map to genes or exones using the the RPKM (Reads per Kilobases per Million reads) methods.
|
24 months
|
協力者と研究者
スポンサー
捜査官
- 主任研究者：Dagmar F. Bunæs, PhD、Department of Clinical Dentistry - Periodontics, University of Bergen, NORWAY
出版物と役立つリンク
一般刊行物
- Bunaes DF, Lie SA, Enersen M, Aastrom AN, Mustafa K, Leknes KN. Site-specific treatment outcome in smokers following non-surgical and surgical periodontal therapy. J Clin Periodontol. 2015 Oct;42(10):933-42. doi: 10.1111/jcpe.12462. Epub 2015 Oct 28.
- Bunaes DF, Mustafa M, Mohamed HG, Lie SA, Leknes KN. The effect of smoking on inflammatory and bone remodeling markers in gingival crevicular fluid and subgingival microbiota following periodontal therapy. J Periodontal Res. 2017 Aug;52(4):713-724. doi: 10.1111/jre.12438. Epub 2017 Mar 17.
研究記録日
主要日程の研究
研究開始 (実際)
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (実際)
学習記録の更新
投稿された最後の更新 (実際)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
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