Functional and Metabolic Effects of Ketone Bodies on Human Atrial Tissue in Patients With and Without Heart Failure
研究概览
详细说明
Ketone bodies are high energy molecules which are naturally occurring in the body during fasting or stress.
Recently, ketone bodies have been shown to improve cardiac function in patients with heart failure(1) through a mechanism, which has yet to be elaborated.
We wish to evaluate the effects of ketone body administration on cardiac tissue subjected to ischemia reperfusion injury. We will evaluate contractile force of the and the mitochondrial performance. Cardiac atrial tissue will be obtained from patients undergoing coronary artery bypass grafting (CABG) or valve replacement surgery. Patients will be recruited after signing an informed consent and allocated in heart failure / non-heart failure groups based on a clinical evaluation.
研究类型
注册 (预期的)
阶段
- 不适用
联系人和位置
学习联系方式
- 姓名：Jacob Seefeldt, B.M
- 电话号码：+45 41178633
- 邮箱：jacob.seefeldt@clin.au.dk
研究联系人备份
- 姓名：Bent Roni Nielsen, M.D., PhD.
- 电话号码：+45 51 21 93 63
- 邮箱：bent.nils@midt.rm.dk
学习地点
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Aarhus、丹麦、8200
- Aarhus University Hospital
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接触：
- Jacob Seefeldt, M.B.
- 电话号码：+45 4117633
- 邮箱：jacob.seefeldt@clin.au.dk
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接触：
- Bent Roni Nielsen, M.D., Ph.D.
- 电话号码：+45 51219363
- 邮箱：bent.nils@midt.rm.dk
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首席研究员：
- Bent Roni Nielsen, M.D., PhD
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副研究员：
- Jacob Seefeldt, M.B
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
描述
Inclusion Criteria:
- EF < / > 45%, hospitalized for elective CABG or heart valve surgery
Exclusion Criteria:
- Myocardial infarction within 4 weeks, atrial fibrillation, medically treated thyroid disease, oral treatment with opioids.
学习计划
研究是如何设计的？
设计细节
- 主要用途：基础科学
- 分配：非随机化
- 介入模型：并行分配
- 屏蔽：无（打开标签）
武器和干预
参与者组/臂 |
干预/治疗 |
|---|---|
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其他：Heart failure
Ejection fraction < 45%
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3-hydroxybuturate will be administered directly as a modified Krebs Henseleit buffer in a super perfused atrial strips model
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其他：Non-heart failure
Ejection fraction > 45%
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3-hydroxybuturate will be administered directly as a modified Krebs Henseleit buffer in a super perfused atrial strips model
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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Contractile force recovery
大体时间：Outcome will be assessed within a year when the inclusion of patients is complete
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Atrial strips model, contractile force (nm*mm^-1) during stabilization, ischemia and reperfusion
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Outcome will be assessed within a year when the inclusion of patients is complete
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
Mitochondrial respiratory capacity
大体时间：Outcome will be assessed within a year when the inclusion of patients is complete
|
High Resolution Respirometry, Complex I and Complex I+II respiration (pmol O2 · s-1·mg-1) assessed via a substrate and inhibitor (SUIT) protocol.
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Outcome will be assessed within a year when the inclusion of patients is complete
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Mitochondrial Reactive Oxygen Species production
大体时间：Outcome will be assessed within a year when the inclusion of patients is complete
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High Resolution FluoRespirometry, Complex I and Complex I+II linked ROS generation measured with AmplexUltra red and H2O2 interaction fluorescense
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Outcome will be assessed within a year when the inclusion of patients is complete
|
合作者和调查者
调查人员
- 研究主任：Hans Erik Bøtker, M.D., Prof.、Aarhus University Hospital
出版物和有用的链接
研究记录日期
研究主要日期
学习开始 (预期的)
初级完成 (预期的)
研究完成 (预期的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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