Bioavailability and Metabolism of Anthocyanins Following Acute Cranberry Consumption

Bioavailability and Metabolism of Anthocyanins Following Acute Cranberry (Vaccinium Macrocarpon)Consumption

This study hypothesis is that anthocyanins from cranberry juice are bioavailable and can be measured in blood and urine after a single acute consumption of cranberry juice.

Study Overview

• Status: Completed
• Conditions: Cardiovascular Disease; Bioavailability
• Intervention / Treatment: Dietary supplement: Experimental

Detailed Description

Little is known about the acute or chronic pharmacokinetics and metabolism of anthocyanins consumed in amounts relevant to usual dietary intakes. Thus, we hypothesize that anthocyanins from a single oral dose of cranberry juice will be absorbed quickly and can be measured in the circulation and urine within 2-4 h. Further, these anthocyanins will undergo a degree of metabolism but will circulate as well as be removed from blood as both parent compounds and metabolic products.

This research proposal is submitted as an independent sub-study of an existing project, "Effects of Cranberry Juice on Endothelial Function in Patients with Coronary Artery Disease", conducted by Joseph A. Vita, MD at the Boston University School of Medicine and sponsored by Ocean Spray Cranberries, Inc.

This study includes 15 subjects aged 21-80 y and with demonstrated endothelial dysfunction and angiographically proven coronary artery disease who will receive a single dose of 480 mL cranberry juice.

To test the hypotheses of the study to be conducted at HNRCA Tufts, we will utilize blood and urine samples that are already collected and stored. We will separate and analyze anthocyanins in these samples by HPLC and determine the pharmacokinetics of relevant anthocyanins.

• Study Type: Observational
• Enrollment (Actual): 15

Contacts and Locations

Study Locations

• United States
  • Massachusetts
    • Boston, Massachusetts, United States, 02111
      • Jean Mayer USDA Human Nutrition Center on Aging at Tufts University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 21 years to 80 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

Subjects aged 21-80 y and with demonstrated endothelial dysfunction and angiographically proven coronary artery disease

Description

Inclusion Criteria:

• Stable CAD Patients
• Men & postmenopausal women
• Aged 21-80 years
• All Ethnic Groups
• English Language

Exclusion Criteria:

• Subjects with heart failure are not eligible for participation in this study.
• Women with a positive urine beta HCG pregnancy test and lactating women or women who are planning to become pregnant.
• Clinical history of other major illness including end-stage cancer, renal failure, hepatic failure, gastrointestinal disorders that may impair absorption, or other conditions that in the opinion of the principal investigator make a clinical study inappropriate.
• Regular use of oral steroids
• Cigarette smoking and/or nicotine replacement use
• Regular daily intake of ≥ 2 alcoholic drinks
• Illicit drug use
• History of a psychological illness or condition such as to interfere with the subject's ability to understand the requirements of the study.
• No dietary supplements containing phenolic compounds, i.e., herbal preparations, or berry containing preparations (such as cranberry capsules) for one month prior to study admission.
• Treatment with an investigational new drug within the last 30 days.

Study Plan

How is the study designed?

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Dietary Supplement; Cranberry Juice Consumption	Dietary supplement: Experimental; Administration of of 480 mL cranberry juice; Other Names: Cranberry Juice

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Anthocyanin levels in plasma and urine	0, 2, and 4 hours

Collaborators and Investigators

• Sponsor: Tufts University
• Investigators: Principal Investigator: Paul E. Milbury, Ph.D., Tufts University

Study record dates

Study Major Dates

• Study Start: June 1, 2007
• Primary Completion (Actual): April 1, 2008
• Study Completion (Actual): April 1, 2008

Study Registration Dates

• First Submitted: December 15, 2008
• First Submitted That Met QC Criteria: December 16, 2008
• First Posted (Estimate): December 17, 2008

Study Record Updates

• Last Update Posted (Estimate): December 2, 2009
• Last Update Submitted That Met QC Criteria: December 1, 2009
• Last Verified: December 1, 2008

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Cardiovascular Diseases
• Other Study ID Numbers: Tufts 7903