Effect of Partial Sleep Deprivation on Immunological System in Peripheral Blood

Effect of Sleep Deprivation on Expression of T Cell Markers in Peripheral Blood of Medical Residents

Sleep deprivation was found to affect many organs including the immune system and predisposing for various health consequences including diabetes hypertension infections and increase in neoplastic diseases.

Subjects will be evaluated for immune parameters in peripheral blood test following a regular nigh sleep and compared with a test performed following a night shift in which they slept less than 3 hours.

Each participant will serve as its own control.

Study Overview

• Status: Unknown
• Conditions: Sleep Deprivation
• Intervention / Treatment: Other: Blood withdrawal

Detailed Description

Overall Study Design and Plan

1. Subjects will be bled on the day of the experiment between 8and 9 AM.
2. Blood tubes will be tested for T cell markers, serum cytokines levels, and other immune parameters by western blots.
3. The first tube will be tested on the day of experiment.
4. The second tube will kept in the lab and will be tested 24 hours later.
5. The third tube will be kept in the pocket of the subject and be tested 24 hours later.
6. The fourth tube will be kept in the pocket of another subject and will be tested 24 hours later.
7. The subject will be tested again following 24 hours.
8. Each subject will be tested twice following a regular 6-8 hours of sleep, and following an on call duty in which he slept less than 3 hours.

• Study Type: Observational
• Enrollment (Anticipated): 50

Contacts and Locations

Study Locations

• Israel
  • Jerusalem, Israel, 91120
    • Hadassah Medical Organization

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

Medical residnets

Description

Inclusion Criteria:

Male and female ages 18-40 signed confirmed consent no evidence of chronic disease or chronic medications

Exclusion Criteria:

Any type of acute infection or other acute illness

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Control
• Time Perspectives: Prospective

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
surface markers on T cells as assessed by FACS	Other: Blood withdrawal; Blood withdrawal

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
surface markers of T cell as assessed by FACS	6 months
serum cytokine levels	6 months
immune parameters to be assessed by western including STAT proteins	6 months

Collaborators and Investigators

• Sponsor: Hadassah Medical Organization

Study record dates

Study Major Dates

• Study Start: September 1, 2013
• Primary Completion (Anticipated): September 1, 2014
• Study Completion (Anticipated): September 1, 2014

Study Registration Dates

• First Submitted: August 25, 2013
• First Submitted That Met QC Criteria: August 27, 2013
• First Posted (Estimate): August 28, 2013

Study Record Updates

• Last Update Posted (Estimate): August 28, 2013
• Last Update Submitted That Met QC Criteria: August 27, 2013
• Last Verified: August 1, 2013

More Information

Terms related to this study

• Keywords: T cell markers
• Additional Relevant MeSH Terms: Mental Disorders; Nervous System Diseases; Dyssomnias; Sleep Wake Disorders; Neurologic Manifestations; Sleep Deprivation
• Other Study ID Numbers: LDB001

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No