Protein Requirements for Active Adolescent Males (IAAO-AM)

Evaluation of Protein Requirements in Active Adolescent Males

Protein requirements in active individuals have been suggested to be greater than the current recommended dietary allowance (RDA).

Nutritional requirements for dietary amino acids in adolescents have traditionally been determined utilizing the nitrogen balance technique, which is prone to underestimating protein requirements. As a result, there is a need to re-evaluate recommendations in order to characterize how dietary amino acid needs may be modulated by physical activity.

Recent studies using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 50% higher than the RDA based on nitrogen balance data.

The purpose of this study is to measure the protein requirement in adolescent, males using the IAAO technique in the presence of a variable intensity exercise stimulus.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Dietary supplement: Amino Acid Intake

• Study Type: Interventional
• Enrollment (Actual): 7
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Canada
  • Ontario
    • Toronto, Ontario, Canada, M5S2C9
      • Goldring Centre for High Performance Sport

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 10 years to 15 years (Child)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Male

Description

Inclusion Criteria (Adolescents):

• Healthy, active adolescent who regularly participates in moderate to vigorous physical activity 5 times a week for a minimum of 1 hour.
• Being -0.5 to +1.0 years from peak height velocity, determined by the ratio of standing to sitting height, with the maximal age for adolescents being 16 years.
• Obtaining a minimum level of 8.3 on the Beep Test

Exclusion Criteria (All Subjects):

• Inability to meet health and physical activity guidelines according to the Physical activity readiness questionnaire (PAR-Q+) and Physical Activity Questionnaire,
• an inability to adhere to any of the protocol guidelines (i.e. caffeine consumption)
• a biological age falling outside of the aforementioned ranges

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Active, Adolescent males; Subjects will receive different levels of amino acid intakes, varying from 0.2-2.67 g/kg/d	Dietary supplement: Amino Acid Intake; Amino acid intakes will be varied at 0.2-2.67 g/kg/d

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
13CO2 ([13]Carbon Dioxide) Excretion (µmol/kg/h)	Each trial day will assess a different protein intake with 13CO2 excretion defining the level of protein adequacy. Each trial day will be separated by ~1wk washout with all trial being completed in approximately 3 months.	4 hours per study day

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
[13C]Phenylalanine Oxidation (µmol/kg/h)	Each trial day will assess a different protein intake with 13CO2 excretion defining the level phenylalanine oxidation being used as a secondary marker of protein adequacy. Each trial day will be separated by ~1wk washout with all trial being completed in approximately 3 months.	4 hours per study day

Collaborators and Investigators

• Sponsor: University of Toronto
• Collaborators: Ajinomoto Co., Inc.
• Investigators: Principal Investigator: Daniel Moore, PhD, University of Toronto

Study record dates

Study Major Dates

• Study Start (Actual): October 1, 2016
• Primary Completion (Actual): November 15, 2017
• Study Completion (Actual): April 30, 2018

Study Registration Dates

• First Submitted: June 10, 2016
• First Submitted That Met QC Criteria: August 3, 2016
• First Posted (Estimate): August 8, 2016

Study Record Updates

• Last Update Posted (Actual): March 29, 2019
• Last Update Submitted That Met QC Criteria: March 28, 2019
• Last Verified: March 1, 2019

More Information

Terms related to this study

• Keywords: Increased Metabolic Requirement
• Other Study ID Numbers: IAAO-AM

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No