Clinical and Biochemical Evaluation of Dental and Periodontal Conditions in Children With Juvenile Idiopathic Arthritis

Aimed to be done in the planned thesis to evaluate the dental and periodontal health of patients with newly diagnosed JIA and healthy-periodontal problems with cytokines from saliva and oxidative stress markers non-invasively, and thus, to determine the markers' evaluability in terms of markers in determining the state of inflammation among individuals with and without the disease.

Study Overview

• Status: Active, not recruiting
• Conditions: Gingivitis; Juvenile Idiopathic Arthritis; Decay, Dental
• Intervention / Treatment: Diagnostic test: Humans ELISA kits

Detailed Description

Periodontal diseases and inflammatory diseases such as arthritis show similar clinical pathologies such as connective tissue deterioration and bone destruction. They carry the risk of being seen together and the possibility of mutual influence. It is important whether these patients have a difference in cytokine profile from individuals who do not have the disease. In the literature, it has been determined that pro and anti-inflammatory cytokines, which are involved in the pathogenesis of periodontitis, are also involved in the pathogenesis of juvenile idiopathic arthritis (JIA). Cytokines play an undeniable role in the pathogenesis of both diseases. The cytokine profile in periodontal disease is distinctly similar to that of rheumatoid arthritis (RA). As in RA, the level of pro-inflammatory cytokines such as interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) increase in periodontal disease. Again, it is stated that oxidative stress assessment is an important factor in evaluating the development of the disease. Saliva evaluation can be made with new oxidative stress markers that can be used in this sense.

On the subject of the proposed thesis; It is to determine the profile of dental and periodontal health in a specific group in terms of biochemical and microbiology using different body fluids together with clinical findings and has an interdisciplinary character. Aimed to be done in the planned thesis;

It was aimed to evaluate the dental and periodontal health of patients with newly diagnosed JIA and healthy-periodontal problems with cytokines from saliva and oxidative stress markers non-invasively, and thus, to determine the markers' evaluability in terms of markers in determining the state of inflammation among individuals with and without the disease.

• Study Type: Observational
• Enrollment (Anticipated): 114

Contacts and Locations

Study Locations

• Turkey
  • Maltepe
    • Istanbul, Maltepe, Turkey
      • Marmara University Faculty of Dentistry

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 5 years to 16 years (CHILD)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

Patients who have no disease other than juvenile idiopathic arthritis, newly diagnosed and not yet using medication.

Description

Inclusion Criteria:

• healty patients

Exclusion Criteria:

• used antibiotics within the last 3 months

Study Plan

How is the study designed?

Number of groups / cohorts

4

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
patient with juvenile idiopathic arthritis and gingivitis; It is a group of patients between the ages of 5 and 16 who have recently been diagnosed with juvenile idiopathic arthritis and gingivitis, have not used medication yet.	Diagnostic test: Humans ELISA kits; ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The saliva of the patients included in the study will be examined with ELISA kits after they are taken.
healthy patient group with gingivitis; It is a group of healthy patients with gingivitis between the ages of 5-16 who have not taken antibiotics in the last 3 months.	Diagnostic test: Humans ELISA kits; ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The saliva of the patients included in the study will be examined with ELISA kits after they are taken.
healthy patient group without gingivitis; It is a group of healthy patients without gingivitis between the ages of 5-16 who have not taken antibiotics in the last 3 months.	Diagnostic test: Humans ELISA kits; ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The saliva of the patients included in the study will be examined with ELISA kits after they are taken.
patient with juvenile idiopathic arthritis and without gingivitis; It is a group of patients between the ages of 5 and 16 who have recently been diagnosed with juvenile idiopathic arthritis and without gingivitis, have not used medication yet.	Diagnostic test: Humans ELISA kits; ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. The saliva of the patients included in the study will be examined with ELISA kits after they are taken.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
biochemical evaluation of saliva in juvenile idiopathic arthritis	Saliva samples were taken from the patients and the levels of interleukin-17, TNF alpha and Total antioxidant status in the saliva were measured with ELISA kits.	baseline

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Evaluation of oral hygiene in patients with juvenile idiopathic arthritis	The number of caries in the patient was evaluated with the DMFT-dft index.	baseline
Evaluation of periodontal health in patients with juvenile idiopathic arthritis	Silness-Loe plaque index (PI) was used to assess periodontal health.	baseline
Examination of periodontal health in patients with juvenile idiopathic arthritis	Gingival index (GI) was also used to examination periodontal health.	baseline
periodontal health in patients with juvenile idiopathic arthritis	Bleeding on prob index were used for periodontal health examination.	baseline

Collaborators and Investigators

• Sponsor: Marmara University
• Investigators: Study Chair: Betul Sozeri, Researcher; Study Chair: Ferhat Demir, Researcher; Study Chair: Selin Yıldırım, Researcher

Study record dates

Study Major Dates

• Study Start (ACTUAL): May 6, 2020
• Primary Completion (ANTICIPATED): May 15, 2022
• Study Completion (ANTICIPATED): September 1, 2022

Study Registration Dates

• First Submitted: January 21, 2022
• First Submitted That Met QC Criteria: January 30, 2022
• First Posted (ACTUAL): February 1, 2022

Study Record Updates

• Last Update Posted (ACTUAL): February 21, 2022
• Last Update Submitted That Met QC Criteria: February 18, 2022
• Last Verified: January 1, 2022

More Information

Terms related to this study

• Keywords: juvenile idiopathic arthritis; periodontal status; pediatric dentistry
• Additional Relevant MeSH Terms: Infections; Immune System Diseases; Autoimmune Diseases; Joint Diseases; Musculoskeletal Diseases; Rheumatic Diseases; Connective Tissue Diseases; Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Gingival Diseases; Tooth Demineralization; Tooth Diseases; Arthritis; Gingivitis; Dental Caries; Arthritis, Juvenile
• Other Study ID Numbers: 2019-146

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No