Detection of Circulating Tumor DNA After Stereotactic Ablative Radiotherapy in Patients With Unbiopsied Lung Tumors (SABR-DETECT)

The goal of this observational study is to determine if liquid biopsies from patients with stage I non-small cell lung cancer (NSCLC) can add to the diagnosis of a small lung cancer, or can better detect recurrent lung cancer compared to the standard of care procedures used for diagnosing this type of cancer. The main question[s] it aims to answer are:

• Primary Objective:

  1) To assess whether liquid biopsy for molecular residual disease during follow-up can predict a recurrence of lung cancer

• Secondary Objectives:

  1. To assess the impact of SABR on detection rates of ctDNA in patients undergoing SABR for early-stage lung tumors.
  2. To correlate positivity by blood-based cancer detection platforms and pre-treatment probability of malignancy using the Brock and Herder models.

Study investigators will also assess the rate of detection for targetable mutations in this patient population, and to correlate ctDNA findings, in patients without tissue confirmed disease.

Blood samples from participants will collected at eight (8) time-points: before the participant's first radiation treatment, following their first treatment and then at their 3-month, 6-month, 9-month, 12-month, 18-month and 24-month follow-up visits.

Study Overview

• Status: Recruiting
• Conditions: Non Small Cell Lung Cancer

Detailed Description

This is a multi-institutional cohort study. The first cohort will be comprised of patients with suspected stage I/IIA NSCLC with plans to undergo curative SABR. The second cohort will be comprised of patients with biopsy proven NSCLC, with clinically staged I/IIA disease with a plan to undergo definitive therapy with SABR.

Focused, or stereotactic, radiation therapy is a standard treatment for early-stage lung cancer when surgery is not possible due to comorbidities or when patient denies surgery and opts for radiation therapy. Many patients with early stage (1 or 2) disease often have treatment with radiation without a tissue biopsy, because of the risk associated with the procedure of tissue biopsy. If the chances of mass being spread to other organs are very high, the risk of treating a lesion with radiation without a biopsy is less than the risk of a biopsy. Unfortunately, many of these patients will have their cancer come back at the local site or at a new site (10-20% risk of recurrence at 1 year). At such time they need a tissue biopsy for diagnosis and biomarker testing to decide the best treatment options. The time to get a biopsy and complete results can take over a month.

Liquid biopsy is a type of test which isolates the cancer derived circulating DNA from blood. This DNA can be tested for mutations and other changes. This offers a chance to diagnose cancer patients in whom biopsy is not possible, or may provide the result faster or safer than a conventional biopsy. Additionally, liquid biopsies can detect mutations which can be used to guide treatment if cancer comes back (recurs); and treatment could be started without a biopsy or immediately after the biopsy before the results are available. It may also help detect a recurrence earlier compared to surveillance imaging.

This study, SABR-DETECT may answer these questions -

1. Can the investigators detect a recurrence earlier with a liquid biopsy, compared to standard surveillance with CT scans?
2. Can radiation increase the ability to diagnose cancer when the baseline liquid biopsy test is negative?
3. Can liquid biopsy be used for diagnosis of lung cancer in patients when a tissue biopsy is not possible or the risks are too high?

Plasma will be collected for ctDNA and cancer detection analysis at eight time-points. At each time-point, four 10 mL (Paxgene ccfDNA, Streck BCT or K2EDTA) tubes will be drawn.

• Draw #1: prior to the first fraction of SABR, ideally on the same day as treatment (but before treatment delivery).
• Draw #2: this should be collected on the second day after the first fraction (± 24 hours if collection is not feasible on that day). For example, if the first treatment is on a Monday, the second collection should occur on a Wednesday, but may occur anytime between Tuesday and Thursday.
• Draws #3-8: at 3-month, 6-month, 9-month, 12-month, 18-month and 24-month follow-up, respectively.

• Study Type: Observational
• Enrollment (Estimated): 100

Contacts and Locations

• Study Contact: Name: David Palma, MD; Phone Number: 519-685-8500; Email: David.Palma@lhsc.on.ca
• Study Contact Backup: Name: Daniel Breadner, MD; Phone Number: 519-685-8640; Email: Daniel.Breadner@lhsc.on.ca

Study Locations

• Canada
  • Ontario
    • London, Ontario, Canada, N6A 5W9
      • Recruiting
      • London Health Sciences Centre

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: No

• Sampling Method: Non-Probability Sample

Study Population

Patients diagnosed with Stage I/IIA Non-small cell lung cancer that will undergo Stereotactic Ablative Radiotherapy (SABR) for a solitary pulmonary nodule with tissue confirmation or those without tissue confirmation of malignancy.

Description

Inclusion Criteria:

• Age 18 years or older
• Willing to provide informed consent
• Undergoing SABR for a solitary pulmonary nodule (SPN) with tissue confirmation or those without tissue confirmation of malignancy, must have a pretreatment likelihood of malignancy of ≥ 60% using either the Herder or Brock models (60% probability was chosen to have a reasonable chance that there will indeed be cancer in the nodule; however, most patients are expected to have a pretreatment probability of > 85%).
• Tumor stage T1-T2b (≤ 5 cm)
• No evidence of nodal or distant metastases
• Eastern Cooperative Oncology Group (ECOG) performance status 0-3

Exclusion Criteria:

• Contraindications to radiotherapy
• Prior history of any invasive malignancy within 5 years, which might interfere with the interpretation of the ctDNA results. Non-melanoma skin cancer is allowed if under appropriate control.

Study Plan

How is the study designed?

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort
Patients with Unbiopsied, Presumed Stage I/IIA NSCLC Undergoing SABR; The first cohort will be comprised of patients with suspected stage I/IIA NSCLC with plans to undergo curative SABR.
Patients with Stage I-IIA NSCLC Undergoing SABR; The second cohort will be comprised of patients with biopsy proven NSCLC, with clinically staged I/IIA disease with a plan to undergo definitive therapy with SABR.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
To assess whether liquid biopsy for molecular residual disease during follow-up can predict a recurrence of lung cancer.	Percentage of patients with MRD detected prior to or at the time of radiological recurrence, with longitudinal monitoring of ctDNA	End of study (approximately Dec 2023)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
To assess the impact of SABR on detection rates of ctDNA in patients undergoing SABR for early-stage lung tumors.	The percentage of patients with undetectable ctDNA at baseline who then develop detectable ctDNA after one fraction of SABR.; Increase in variant allelic frequency (vAF) or quantifiable ctDNA (mutant molecules/mL of plasma) from baseline to post-treatment samples, in patients with detectable ctDNA at baseline.	End of study (approximately Dec 2023)
To correlate positivity by blood-based cancer detection platforms and pre-treatment probability of malignancy using the Brock and Herder models.	Correlation between pre-treatment malignancy risk to the rate of ctDNA positivity	End of study (approximately Dec 2023)

Collaborators and Investigators

• Sponsor: Lawson Health Research Institute
• Investigators: Principal Investigator: Daniel Breadner, MD, 519-685-8640

Study record dates

Study Major Dates

• Study Start (Actual): April 3, 2023
• Primary Completion (Estimated): December 1, 2023
• Study Completion (Estimated): December 1, 2023

Study Registration Dates

• First Submitted: February 15, 2023
• First Submitted That Met QC Criteria: June 19, 2023
• First Posted (Actual): June 27, 2023

Study Record Updates

• Last Update Posted (Actual): June 27, 2023
• Last Update Submitted That Met QC Criteria: June 19, 2023
• Last Verified: June 1, 2023

More Information

Terms related to this study

• Keywords: Early Stage Lung Cancer; Stage I/IIA; Tumor stage T1-T2b
• Additional Relevant MeSH Terms: Respiratory Tract Diseases; Neoplasms; Lung Diseases; Neoplasms by Site; Respiratory Tract Neoplasms; Thoracic Neoplasms; Carcinoma, Bronchogenic; Bronchial Neoplasms; Lung Neoplasms; Carcinoma, Non-Small-Cell Lung
• Other Study ID Numbers: SABR-DETECT

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No