Macrophages Effect on Chemoresistance
Impact of Macrophages on De-Novo Chemoresistance of Adenocarcinoma Cells
Macrophages are derived from monocytes recruited to the tumor site and stimulated by specific chemokines secreted by tumor cells. These tumor associated macrophages (TAMs) have been postulated as being involved in the progression of cancer.
Based on our preliminary findings and on published data we hypothesized that macrophage-induced chemoresistance (MIC) can promote survival of pancreatic carcinoma cells during chemotherapy.
The overall goal of this study is to evaluate the mechanism of MIC in an in-vitro model of Pancreatic ductal adenocarcinoma (PDA).
methods:
- The human PDA cell line Panc1 will be grown in suitable conditions.
- Macrophages will be produced by incubating mononuclear cells from the blood of healthy donors in medium with M-CSF for 7 days.
- TAMs will be generated by culturing these macrophages with tumor-culture conditioning medium (TCCM) of PDA Cells for an additional 72 hours.
- Human pancreatic cells (PANC1) will be treated with gemcitabine following exposure to macrophages CM.
- Cell proliferation will be quantified by light microscopy and by an XTT Cell Proliferation Assay Kit.
Studieoversigt
Status
Intervention / Behandling
Undersøgelsestype
Tilmelding (Forventet)
Fase
- Ikke anvendelig
Deltagelseskriterier
Berettigelseskriterier
Aldre berettiget til at studere
Tager imod sunde frivillige
Køn, der er berettiget til at studere
Beskrivelse
Inclusion Criteria:
- healthy people
Exclusion Criteria:
- refusal to participate
Studieplan
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
- Primært formål: Grundvidenskab
- Tildeling: N/A
- Interventionel model: Enkelt gruppeopgave
- Maskning: Ingen (Åben etiket)
Våben og indgreb
Deltagergruppe / Arm |
Intervention / Behandling |
|---|---|
|
Andet: irrelevant
|
Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
|---|---|---|
|
proliferation and migration of PANC1 cell line in the tested groups.
Tidsramme: one day to get the blood samples, and a year after that to complete the basic research
|
the macrophages will be isolated from the blood we'll get from the healthy volunteers.
the outcome measure is related to the basic research and not clinical so there's no symptom/outcome related to the participants.
|
one day to get the blood samples, and a year after that to complete the basic research
|
Sekundære resultatmål
Resultatmål |
Tidsramme |
|---|---|
|
To determine whether direct cell-to-cell interaction or soluble factors participate in MIC.
Tidsramme: a year from obtaining the blood samples
|
a year from obtaining the blood samples
|
Samarbejdspartnere og efterforskere
Sponsor
Datoer for undersøgelser
Studer store datoer
Studiestart
Primær færdiggørelse (Forventet)
Datoer for studieregistrering
Først indsendt
Først indsendt, der opfyldte QC-kriterier
Først opslået (Skøn)
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Skøn)
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
Sidst verificeret
Mere information
Begreber relateret til denne undersøgelse
Nøgleord
Yderligere relevante MeSH-vilkår
Andre undersøgelses-id-numre
- Pending (Clinical Research Information Service)
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