Microbiological and Biochemical Profiling of Amniotic Fluid in Women With Short Cervix or Premature Rupture of Membranes (CIPRO)

Inclusion Criteria:

• Singleton pregnancy;
• Age > 18 years;
• Pregnant women diagnosed with cervical incompetence (via ultrasound criteria) with cervical length <24 mm, before 24 weeks of gestational age, with intact amniotic membranes and absence of uterine contractions;
• Pregnant women diagnosed with p-PROM (via clinical criteria and/or with a qualitative immunoassay vaginal test) before 34 weeks of gestational age;
• Healthy pregnant women with uncomplicated obstetric history who underwent amniocentesis to exclude chromosomal abnormalities;
• Informed consent to participate in the study.

Exclusion Criteria:

• Multiple pregnancy;
• Patients with contraindications to amniocentesis, including severe bleeding disorders or placenta previa;
• Patients with known fetal anomalies incompatible with life;
• Patients with sepsis or clinical diagnosis of chorioamnionitis;