Respiratory Pathogen Distribution During and After the COVID-19 Pandemic
Comparative Analysis of Respiratory Pathogens During and After the SARS-CoV-2 Pandemic: A Retrospective Multiplex PCR-Based Study
This retrospective observational study aims to evaluate changes in the prevalence and distribution of respiratory pathogens detected by multiplex respiratory polymerase chain reaction (PCR) testing during and after the SARS-CoV-2 pandemic. The study includes 537 patients presenting with respiratory infection symptoms at a tertiary care center. Patients were divided into pandemic-period and post-pandemic-period groups, and respiratory pathogen profiles were compared between the groups.
The study investigates the epidemiological impact of COVID-19-related public health measures on respiratory pathogen circulation patterns, including bacterial and viral respiratory infections. The findings may contribute to improved respiratory infection surveillance, diagnostic strategies, and post-pandemic public health preparedness.
調査の概要
状態
詳細な説明
The COVID-19 pandemic significantly altered the epidemiology of respiratory infections worldwide. Non-pharmaceutical interventions (NPIs), including mask use, social distancing, travel restrictions, enhanced hygiene measures, and lockdown policies, were implemented primarily to reduce SARS-CoV-2 transmission. However, these interventions also affected the circulation patterns of other respiratory pathogens.
Several studies have demonstrated substantial reductions in influenza, respiratory syncytial virus (RSV), and other respiratory viral infections during periods of strict public health measures. Following the relaxation of these interventions, many regions experienced re-emergence and altered seasonal patterns of respiratory pathogens. Continuous surveillance of respiratory infections has therefore become increasingly important for both clinical management and public health preparedness.
This retrospective observational cohort study aims to evaluate changes in respiratory pathogen prevalence during and after the SARS-CoV-2 pandemic using multiplex respiratory PCR testing data obtained from patients presenting with respiratory infection symptoms at a tertiary care center. A total of 537 patients were included and categorized into pandemic-period and post-pandemic-period groups.
Demographic characteristics and multiplex PCR results were analyzed and compared between the groups. Respiratory pathogens evaluated in the study included bacterial and viral agents such as Streptococcus pneumoniae, Haemophilus influenzae, Influenza A/B, SARS-CoV-2, RSV, Rhinovirus, Bocavirus, Parainfluenza virus, Coronavirus, and Metapneumovirus.
The study aims to contribute to understanding the long-term epidemiological effects of the COVID-19 pandemic and associated public health measures on respiratory pathogen distribution. In addition, the findings may support future infection surveillance strategies, diagnostic approaches, and healthcare preparedness in post-pandemic periods.
研究の種類
入学 (実際)
連絡先と場所
研究場所
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Istanbul、トルコ（Türkiye）、34768
- Hisar Intercontinental Hospital
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参加基準
適格基準
就学可能な年齢
- 子
- 大人
- 高齢者
健康ボランティアの受け入れ
サンプリング方法
調査対象母集団
説明
Inclusion Criteria:
- Adults and/or pediatric patients presenting with respiratory infection symptoms
- Patients who underwent multiplex respiratory PCR testing
- Patients with complete demographic and laboratory records
- Patients included during the defined pandemic or post-pandemic study periods
Exclusion Criteria:
- Incomplete laboratory or demographic data
- Duplicate test records from the same infection episode
- Invalid or inconclusive PCR results
- Patients without respiratory infection symptoms
研究計画
研究はどのように設計されていますか？
デザインの詳細
コホートと介入
グループ/コホート |
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Pandemic Period Group
Patients presenting with respiratory infection symptoms who underwent multiplex respiratory PCR testing during the SARS-CoV-2 pandemic period.
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Post-Pandemic Period Group
Patients presenting with respiratory infection symptoms who underwent multiplex respiratory PCR testing during the post-pandemic normalization period.
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この研究は何を測定していますか？
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
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Change in the prevalence of respiratory pathogens between the pandemic and post-pandemic periods
時間枠：Retrospectively assessed at the time of respiratory PCR testing during the study periods. Pandemic period: March 2020 - May 2023 Post-pandemic period: June 2023 - December 202
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The primary outcome is the difference in detection rates of respiratory pathogens identified by multiplex respiratory PCR testing between the SARS-CoV-2 pandemic period and the post-pandemic period.
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Retrospectively assessed at the time of respiratory PCR testing during the study periods. Pandemic period: March 2020 - May 2023 Post-pandemic period: June 2023 - December 202
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協力者と研究者
出版物と役立つリンク
一般刊行物
- Poole S, Brendish NJ, Clark TW. SARS-CoV-2 has displaced other seasonal respiratory viruses: Results from a prospective cohort study. J Infect. 2020 Dec;81(6):966-972. doi: 10.1016/j.jinf.2020.11.010. Epub 2020 Nov 15.
- Bandeira TJPG, Oliveira ALS, Martins LFP, Matos RM, Santos SRNGD, Lopes MC, Sobreira RTP, Rocha HAL. Molecular detection of respiratory viruses: an observational study on respiratory co-infections in children and adults. Braz J Microbiol. 2025 Mar;56(1):537-543. doi: 10.1007/s42770-024-01581-x. Epub 2024 Dec 17.
- Le Hingrat Q, Bouzid D, Choquet C, Laurent O, Lescure FX, Timsit JF, Houhou-Fidouh N, Casalino E, Lucet JC, Descamps D, Visseaux B. Viral epidemiology and SARS-CoV-2 co-infections with other respiratory viruses during the first COVID-19 wave in Paris, France. Influenza Other Respir Viruses. 2021 Jul;15(4):425-428. doi: 10.1111/irv.12853. Epub 2021 Apr 4.
- Olsen SJ, Azziz-Baumgartner E, Budd AP, Brammer L, Sullivan S, Pineda RF, Cohen C, Fry AM. Decreased influenza activity during the COVID-19 pandemic-United States, Australia, Chile, and South Africa, 2020. Am J Transplant. 2020 Dec;20(12):3681-3685. doi: 10.1111/ajt.16381.
- Yam SLS, Baguio JMJ, Chan RWY. Pediatric Respiratory Viral Infection. Viruses. 2024 May 6;16(5):733. doi: 10.3390/v16050733.
- Agca H, Akalin H, Saglik I, Hacimustafaoglu M, Celebi S, Ener B. Changing epidemiology of influenza and other respiratory viruses in the first year of COVID-19 pandemic. J Infect Public Health. 2021 Sep;14(9):1186-1190. doi: 10.1016/j.jiph.2021.08.004. Epub 2021 Aug 8.
- Agha R, Avner JR. Delayed Seasonal RSV Surge Observed During the COVID-19 Pandemic. Pediatrics. 2021 Sep;148(3):e2021052089. doi: 10.1542/peds.2021-052089. Epub 2021 Jun 9. No abstract available.
研究記録日
主要日程の研究
研究開始 (実際)
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (実際)
学習記録の更新
投稿された最後の更新 (実際)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
本研究に関する用語
追加の関連 MeSH 用語
その他の研究ID番号
- HHI-RP-PCR-2026-001
個々の参加者データ (IPD) の計画
個々の参加者データ (IPD) を共有する予定はありますか?
IPD プランの説明
医薬品およびデバイス情報、研究文書
米国FDA規制医薬品の研究
米国FDA規制機器製品の研究
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