Naturally acquired human Plasmodium knowlesi infection, Singapore

Oon Tek Ng, Eng Eong Ooi, Cheng Chuan Lee, Piao Jarrod Lee, Lee Ching Ng, Sze Wong Pei, Tian Ming Tu, Jin Phang Loh, Yee Sin Leo, Oon Tek Ng, Eng Eong Ooi, Cheng Chuan Lee, Piao Jarrod Lee, Lee Ching Ng, Sze Wong Pei, Tian Ming Tu, Jin Phang Loh, Yee Sin Leo

Abstract

We report a case of naturally acquired Plasmodium knowlesi in Singapore, a malaria-free country. Diagnosis was confirmed by PCR with validated species-specific primers. In industrialized countries, free-ranging primates are a potential source of P. knowlesi human infection. P. knowlesi infection is a differential diagnosis of febrile illness acquired in Singapore.

Figures

Figure 1
Figure 1
Patient’s temperature chart showing fever spikes 24 h apart at approximately 7 PM daily (red arrow). The black arrow denotes 38°C, and each blue arrow denotes a difference of 1°C from the neighboring arrow.
Figure 2
Figure 2
Phylogenetic trees comparing our case sample (denoted as SingPk1) with other Plasmodium species, based on SSU rRNA (A) and csp (B) sequences. Species and sequences used were selected to match those previously reported (5). Figures on the branches are bootstrap percentages based on 1,000 replicates, and only those above 80% are shown. GenBank accession numbers are in parentheses.

References

    1. Coatney GR, Collins WE, Warren M, Contacos PG. The primate malarias [CD-ROM; original book published 1971]. Version 1.0. Atlanta: Centers for Disease Control and Prevention; 2003.
    1. Knowles R, Das Gupta BM. A study of monkey-malaria and its experimental transmission to man. Ind Med Gaz. 1932;67:301–20.
    1. Chin W, Contacos PG, Coatney GR, Kimball HR. A naturally acquired quotidian-type malaria in man transferable to monkeys. Science. 1965;149:865. 10.1126/science.149.3686.865
    1. Fong YL, Cadigan FC, Coatney GR. A presumptive case of naturally occurring Plasmodium knowlesi in man in Malaysia. Trans R Soc Trop Med Hyg. 1971;65:839–40. 10.1016/0035-9203(71)90103-9
    1. Singh B, Kim Sung L, Matusop A, Radhakrishnan A, Shamsul SS, Cox-Singh J, et al. A large focus of naturally acquired Plasmodium knowlesi infections in human beings. Lancet. 2004;363:1017–24. 10.1016/S0140-6736(04)15836-4
    1. Jongwutiwes S, Putaporntip C, Iwasaki T, Sata T, Kanbara H. Naturally acquired Plasmodium knowlesi malaria in human, Thailand. Emerg Infect Dis. 2004;10:2211–3.
    1. Zhu HM, Li J, Zheng H. Human natural infection of Plasmodium knowlesi. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006;24:70–1.
    1. Cox-Singh J, Davis TME, Lee KS, Shamsul SSG, Matusop A, Ratnam S, et al. Plasmodium knowlesi malaria in humans is widely distributed and potentially life threatening. Clin Infect Dis. 2008;46:165–71. 10.1086/524888
    1. Goh KT. Eradication of malaria from Singapore. Singapore Med J. 1983;24:255–68.
    1. Chiam PT, Oh HM, Ooi EE. Localised outbreak of Falciparum malaria in Singapore. Singapore Med J. 2003;44:357–8.
    1. Singh B, Bobogare A, Cox-Singh J, Snounou G, Abdullah MS, Rahman HA. A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies. Am J Trop Med Hyg. 1999;60:687–92.
    1. Rougemont M, Van Saanen M, Sahli R, Hinrikson HP, Bille J, Haton K. Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species specific real-time PCR assays. J Clin Microbiol. 2004;42:5636–43. 10.1128/JCM.42.12.5636-5643.2004
    1. McCutchan TF, Kissinger JC, Touray MG, Rogers MJ, Li J, Sullivan M, et al. Comparison of circumsporozoite proteins from avian and mammalian malarias: biological and phylogenetic implications. Proc Natl Acad Sci U S A. 1996;93:11889–94. 10.1073/pnas.93.21.11889
    1. Chin W, Contacos PG, Collins WE, Jether MH, Alpert E. Experimental mosquito-transmission of Plasmodium knowlesi to man and monkey. Am J Trop Med Hyg. 1968;17:355–8.
    1. Yang CMMK. Yong K, Lim KKP. Wild mammals of Singapore. In: Chou LM, PKL Ng. Essays in zoology, papers commemorating the 40th anniversary of the Department of Zoology. Singapore: National University of Singapore; 1990. p. 1–24.

Source: PubMed

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