Bisphenol A in human saliva and urine before and after treatment with dental polymer-based restorative materials

Trine L L Berge, Gunvor B Lygre, Stein A Lie, Christian H Lindh, Lars Björkman, Trine L L Berge, Gunvor B Lygre, Stein A Lie, Christian H Lindh, Lars Björkman

Abstract

The aim of this study was to quantify bisphenol A (BPA) concentrations in saliva and urine before and after treatment with dental polymer-based restorative materials to assess if placement of this material is associated with increased BPA levels in saliva and urine. Twenty individuals in need of at least one dental restoration with polymer-based restorative material were included in this study. The participants were instructed to abstain from eating, drinking, and brushing their teeth for at least 10 h prior to sampling. Saliva and urine were collected before and 10 min (saliva only), 1 h, 24 h, and 1 wk after treatment. Samples were stored at -80°C before analyses. BPA in saliva and urine was determined with liquid chromatography/mass spectrometry. Linear mixed effects regression models were used for statistical analyses. There was a statistically significant increase of salivary BPA concentration directly after placement of the dental polymer-based restorations. Following placement, the concentration of BPA decreased exponentially with time. One week after treatment the BPA level in saliva was only marginally higher than before treatment. In urine, no statistically significant change of the BPA concentration was detected after treatment.

Keywords: composite filling; exposure to BPA; resin-based material; saliva; urine.

Conflict of interest statement

The authors declare no conflicts of interest.

© 2019 The Authors. Eur J Oral Sci published by John Wiley & Sons Ltd.

Figures

Figure 1
Figure 1
Time schedule of saliva (S) and urine (U) sampling.
Figure 2
Figure 2
Salivary concentrations (ng ml−1) of bisphenol A (BPA) among participants (individual patterns are shown) before treatment (baseline), and 10 min, 1 h, 24 h, and 1 wk after treatment with polymer‐based filling material (n = 20).
Figure 3
Figure 3
Concentration (ng ml−1) of bisphenol A (BPA) in saliva after treatment with dental polymer‐based restorative materials as a function of time (h).
Figure 4
Figure 4
Urinary concentrations (ng ml−1) of bisphenol A (BPA) among participants (individual patterns are shown) before treatment (baseline), and 1 h, 24 h, and 1 wk after treatment with polymer‐based filling material (n = 20).

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