Effect of micro-environment maintenance on embryo culture after in-vitro fertilization: comparison of top-load mini incubator and conventional front-load incubator

Abstract

Purpose: To investigate the effect of microenvironment maintenance on embryo culture and clinical results using two types of incubators.

Methods: Temperature and oxygen concentration in a mini-incubator and a conventional incubator were compared following a 5-s door opening/closing procedure. Embryos of 30 in-vitro fertilization embryo transfer (IVF-ET) cases were randomly allocated to either one of the incubator, cultured, and the early-stage good embryo formation rate and the good blastocyst formation rate were compared, as indicators for micro-environment maintenance ability.

Results: Temperature recovery after a 5-s door opening/closing procedure was approximately 5 min for the mini-incubator and 30 min for the conventional incubator. The oxygen concentration return was significantly improved in the mini-incubator (3.0 +/- 0 min) compared with the conventional incubator (7.8 +/- 0.9 min). Both the early-stage good embryo formation rate and the good blastocyst formation rate were significantly higher in the mini-incubator (39.5% and 15.1%) than the conventional incubator (28.4% and 7.8%).

Conclusion: The microenvironment maintenance ability of incubators appears to significantly influence the formation of good embryos.

Figures

Fig. 1

Photograph of top-load mini-incubator and front-load conventional incubator: (A) top-load mini-incubator; (B) front-load conventional incubator

Fig. 2

Embryo and blastcyst of good condition: (A) Early-stage embryo, 4 cells, G3; (B) Early-stage embryo, 8 cells, G3; (C) Blastocyst, 3AB

Fig. 3

Temperature changes of top-load mini-incubator (after opening the door for 5 s)

Fig. 4

Temperature changes of conventional front-load incubator (after opening the door for 5 s)