Oxidized LDL Is Strictly Limited to Hyperthyroidism Irrespective of Fat Feeding in Female Sprague Dawley Rats

Sieglinde Zelzer, Harald Mangge, Sabine Pailer, Herwig Ainoedhofer, Petra Kieslinger, Tatjana Stojakovic, Hubert Scharnagl, Florian Prüller, Daniel Weghuber, Christian Datz, Johannes Haybaeck, Barbara Obermayer-Pietsch, Christian Trummer, Johanna Gostner, Hans-Jürgen Gruber, Sieglinde Zelzer, Harald Mangge, Sabine Pailer, Herwig Ainoedhofer, Petra Kieslinger, Tatjana Stojakovic, Hubert Scharnagl, Florian Prüller, Daniel Weghuber, Christian Datz, Johannes Haybaeck, Barbara Obermayer-Pietsch, Christian Trummer, Johanna Gostner, Hans-Jürgen Gruber

Abstract

Metabolic dysfunctions might play a crucial role in the pathophysiology of thyroid dysfunctions. This study aimed to investigate the impact of a controlled diet (normal versus high fat feeding) on hypothyroid and hyperthyroid Sprague Dawley rats. Female Sprague Dawley rats (n = 66) were grouped into normal diet (n = 30) and high-fat diet (n = 36) groups and subdivided into controls, hypothyroid and hyperthyroid groups, induced through propylthiouracil or triiodothyronine (T3) treatment, respectively. After 12 weeks of treatment metabolic parameters, such as oxidized LDL (oxLDL), malondialdehyde (MDA), 4-hydroxynonenal (HNE), the lipid profile, body weight and food intake parameters were analyzed. Successfully induced thyroid dysfunctions were shown by T3 levels, both under normal and high fat diet. Thyroid dysfunctions were accompanied by changes in calorie intake and body weight as well as in the lipid profile. In detail, hypothyroid rats showed significantly decreased oxLDL levels, whereas hyperthyroid rats showed significantly increased oxLDL levels. These effects were seen under high fat diet and were less pronounced with normal feeding. Taken together, we showed for the first time in female SD rats that only hyper-, but not hypothyroidism, is associated with high atherogenic oxidized LDL irrespective of normal or high-fat diet in Sprague Dawley rats.

Keywords: Sprague Dawley rats; fat feeding; hyperthyroidism; hypothyroidism; metabolic dysfunction; oxidized LDL.

Figures

Figure 1
Figure 1
Box and Whisker Blot of oxidized LDL. Grey bars indicate normal diet and black bars indicate high fat diet. *p < 0.05 compared to appropriate diet control group; ºp < 0.05 compared to normal diet control group.

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