Microbiological characterization in children with aggressive periodontitis

L M Shaddox, H Huang, T Lin, W Hou, P L Harrison, I Aukhil, C B Walker, V Klepac-Ceraj, B J Paster, L M Shaddox, H Huang, T Lin, W Hou, P L Harrison, I Aukhil, C B Walker, V Klepac-Ceraj, B J Paster

Abstract

The objective of this study was to characterize the subgingival microbiota of African-American children with Localized Aggressive Periodontitis (LAP). Fifty-one children were included. Subgingival plaque samples were taken from diseased (DD) and healthy sites (DH) in LAP and from healthy sites in HS and HC and analyzed by 16S rRNA-based microarrays. Aggregatibacter actinomycetemcomitans (Aa) was the only species found to be both more prevalent (OR = 8.3, p = 0.0025) and abundant (p < 0.01) in DD. Filifactor alocis (Fa) was also found to be more prevalent in DD (OR 2.31, CI 1.06-5.01, p = 0.03). Most prevalent species in healthy sites were Selenomonas spp, Veillonella spp, Streptococcus spp, Bergeyella sp, and Kingella oralis. Overall, Streptococcus spp, Campylobacter gracilis, Capnocytophaga granulosa, Haemophilus parainfluenzae, and Lautropia mirabilis were most abundant in healthy children, while Aa, Fa, Tannerella sp, Solobacterium moorei, Parvimonas micra, and Capnocytophaga sp were most abundant in LAP. Based on a comprehensive analysis with 16S rRNA-based microarrays, Aa was strongly associated and site-specific in LAP. In contrast, other species were found to be associated with healthy sites and individuals (ClinicalTrials.gov number CT01330719).

Abbreviations: healthy site in healthy sibling (HS); healthy site in healthy control child (HC).

Trial registration: ClinicalTrials.gov NCT01330719.

Conflict of interest statement

The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

Figures

Figure.
Figure.
Bacterial species which showed significantly different prevalence among sites/groups. DD = diseased sites in children with disease; DH = healthy sites in children with disease; HS = healthy sites in healthy sibling; HC = healthy sites in healthy control children. Significant differences denoted by * DD vs. DH, † DD vs. HS, and ‡DD vs. HC.

References

    1. Alaluusua S, Asikainen S. (1988). Detection and distribution of Actinobacillus actinomycetemcomitans in the primary dentition. J Periodontol 59:504-507
    1. Alfant B, Shaddox LM, Tobler J, Magnusson I, Aukhil I, Walker C. (2008). Matrix metalloproteinase levels in children with aggressive periodontitis. J Periodontol 79:819-826
    1. Armitage GC. (1999). Development of a classification system for periodontal diseases and conditions. Ann Periodontol 4:1-6
    1. Colombo AP, Boches SK, Cotton SL, Goodson JM, Kent R, Haffajee AD, et al. (2009). Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol 80:1421-1432
    1. Colombo AP, Bennet S, Cotton SL, Goodson JM, Kent R, Haffajee AD, et al. (2012). Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and “refractory” subjects by the Human Oral Microbe Identification Microarray (HOMIM). J Periodontol [epub ahead of print 2/10/2012] (in press)
    1. Gajardo M, Silva N, Gomez L, Leon R, Parra B, Contreras A, et al. (2005). Prevalence of periodontopathic bacteria in aggressive periodontitis patients in a Chilean population. J Periodontol 76:289-294
    1. Haffajee AD, Socransky SS, Gunsolley JC. (2003). Systemic anti-infective periodontal therapy. a systematic review. Ann Periodontol 8:115-181
    1. Haubek D, Poulsen K, Westergaard J, Dahlén G, Kilian M. (1996). Highly toxic clone of Actinobacillus actinomycetemcomitans in geographically widespread cases of juvenile periodontitis in adolescents of African origin. J Clin Microbiol 34:1576-1578
    1. Haubek D, Dirienzo JM, Tinoco EM, Westergaard J, Lopez NJ, Chung CP, et al. (1997). Racial tropism of a highly toxic clone of Actinobacillus actinomycetemcomitans associated with juvenile periodontitis. J Clin Microbiol 35:3037-3042
    1. Haubek D, Ennibi OK, Poulsen K, Vaeth M, Poulsen S, Kilian M. (2008). Risk of aggressive periodontitis in adolescent carriers of the JP2 clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans in Morocco: a prospective longitudinal cohort study. Lancet 371:237-242
    1. Ickenstein GW, Klotz JM, Langohr HD. (1999). [Headache caused by Polycythemia vera. Classification of a headache under the heading of metabolic disturbances]. Schmerz 13:279-282 [in German].
    1. Kaldahl WB, Kalkwarf KL, Patil KD, Dyer JK, Bates RE. (1988). Evaluation of four modalities of periodontal therapy. Mean probing depth, probing attachment level and recession changes. J Periodontol 59:783-793
    1. Kaner D, Christan C, Dietrich T, Bernimoulin JP, Kleber BM, Friedmann A. (2007). Timing affects the clinical outcome of adjunctive systemic antibiotic therapy for generalized aggressive periodontitis. J Periodontol 78:1201-1208
    1. Kumar PS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML, Leys EJ. (2003). New bacterial species associated with chronic periodontitis. J Dent Res 82:338-344
    1. Kumar PS, Leys EJ, Bryk JM, Martinez FJ, Moeschberger ML, Griffen AL. (2006). Changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16S cloning and sequencing. J Clin Microbiol 44:3665-3673
    1. Leung WK, Ngai VK, Yau JY, Cheung BP, Tsang PW, Corbet EF. (2005). Characterization of Actinobacillus actinomycetemcomitans isolated from young Chinese aggressive periodontitis patients. J Periodontal Res 40:258-268
    1. Lopez NJ, Mellado JC, Giglio MS, Leighton GX. (1995). Occurrence of certain bacterial species and morphotypes in juvenile periodontitis in Chile. J Periodontol 66:559-567
    1. Mayorga-Fayad I, Lafaurie GI, Contreras A, Castillo DM, Barón A, Aya Mdel R. (2007). [Subgingival microbiota in chronic and aggressive periodontitis in Bogota, Colombia: an epidemiological approach]. Biomedica 27:21-33 [in Spanish].
    1. Offenbacher S. (1996). Periodontal diseases: pathogenesis. Ann Periodontol 1:821-878
    1. Page RC, Kornman KS. (1997). The pathogenesis of human periodontitis: an introduction. Periodontology 2000 14: 9-11
    1. Riep B, Edesi-Neuss L, Claessen F, Skarabis H, Ehmke B, Flemmig TF, et al. (2009). Are putative periodontal pathogens reliable diagnostic markers? J Clin Microbiol 47:1705-1711
    1. Schenkein HA, Barbour SE, Tew JG. (2007). Cytokines and inflammatory factors regulating immunoglobulin production in aggressive periodontitis. Periodontology 2000 45: 113-127
    1. Shaddox L, Wiedey J, Bimstein E, Magnuson I, Clare-Salzler M, Aukhil I, et al. (2010). Hyper-responsive phenotype in localized aggressive periodontitis. J Dent Res 89:143-148
    1. Shaddox LM, Wiedey J, Calderon NL, Magnusson I, Bimstein E, Bidwell JA, et al. (2011). Local inflammatory markers and systemic endotoxin in aggressive periodontitis. J Dent Res 90:1140-1144
    1. Spinozzi G, Langer J. (1999). Spontaneous classification in action by a human-enculturated and language-reared bonobo (Pan paniscus) and common chimpanzees (Pan troglodytes). J Comp Psychol 113:286-296
    1. Stingu CS, Eschrich K, Rodloff AC, Schaumann R, Jentsch H. (2008). Periodontitis is associated with a loss of colonization by Streptococcus sanguinis. J Med Microbiol 57(Pt 4):495-499
    1. Thiha K, Takeuchi Y, Umeda M, Huang Y, Ohnishi M, Ishikawa I. (2007). Identification of periodontopathic bacteria in gingival tissue of Japanese periodontitis patients. Oral Microbiol Immunol 22:201-207
    1. Yang EY, Tanner AC, Milgrom P, Mokeem SA, Riedy CA, Spadafora AT, et al. (2002). Periodontal pathogen detection in gingiva/tooth and tongue flora samples from 18- to 48-month-old children and periodontal status of their mothers. Oral Microbiol Immunol 17:55-59
    1. Yang HW, Huang YF, Chan Y, Chou MY. (2005). Relationship of Actinobacillus actinomycetemcomitans serotypes to periodontal condition: prevalence and proportions in subgingival plaque. Eur J Oral Sci 113:28-33
    1. Zambon JJ, Christersson LA, Slots J. (1983). Actinobacillus actinomycetemcomitans in human periodontal disease. Prevalence in patient groups and distribution of biotypes and serotypes within families. J Periodontol 54:707-711

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