Dual-mode imaging of cutaneous tissue oxygenation and vascular function

Ronald X Xu, Kun Huang, Ruogu Qin, Jiwei Huang, Jeff S Xu, Liya Ding, Urmila S Gnyawali, Gayle M Gordillo, Surya C Gnyawali, Chandan K Sen, Ronald X Xu, Kun Huang, Ruogu Qin, Jiwei Huang, Jeff S Xu, Liya Ding, Urmila S Gnyawali, Gayle M Gordillo, Surya C Gnyawali, Chandan K Sen

Abstract

Accurate assessment of cutaneous tissue oxygenation and vascular function is important for appropriate detection, staging, and treatment of many health disorders such as chronic wounds. We report the development of a dual-mode imaging system for non-invasive and non-contact imaging of cutaneous tissue oxygenation and vascular function. The imaging system integrated an infrared camera, a CCD camera, a liquid crystal tunable filter and a high intensity fiber light source. A Labview interface was programmed for equipment control, synchronization, image acquisition, processing, and visualization. Multispectral images captured by the CCD camera were used to reconstruct the tissue oxygenation map. Dynamic thermographic images captured by the infrared camera were used to reconstruct the vascular function map. Cutaneous tissue oxygenation and vascular function images were co-registered through fiduciary markers. The performance characteristics of the dual-mode image system were tested in humans.

Figures

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References

    1. Myers DE, Anderson LD, Seifert RP, Ortner JP, Cooper CE, Beilman GJ. Noninvasive method for measuring local hemoglobin oxygen saturation in tissue using wide gap second derivative near-infrared spectroscopy. J Biomed Opt. 2005;10(3):034017–034017.
    1. Jarm T, Kragelj R, Liebert A, Lukasiewitz P, Erjavec T, Preseren-Strukelj M. Postocclusive reactive hyperemia in healthy volunteers and patients with peripheral vascular disease measured by three noninvasive methods. Adv Exp Med Biol. 2003;530:661–669.
    1. Treacher DF, Leach RM. Oxygen transport-1. Basic principles. Bmj. 1998;317(7168):1302–1306.
    1. Leach RM, Treacher DF. Oxygen transport-2. Tissue hypoxia. BMJ. 1998;317(7169):1370–1373.
    1. Sen CK. Wound healing essentials: let there be oxygen. Wound Repair Regen. 2009;17(1):1–18.
    1. RL Greenman, Panasyuk S, Wang X, TE Lyons, Dinh T, Longoria L. Early changes in the skin microcirculation and muscle metabolism of the diabetic foot. Lancet. 2005;366(9498):1711–1717.
    1. Valvano JW. The use of thermal diffusivity to quantify tissue perfusion [dissertation] Massachusetts Institute of Technology; 1981.
    1. Hassan M, Togawa T. Observation of skin thermal inertia distribution during reactive hyperaemia using a single-hood measurement system. Physiol Meas. 2001;22(1):187–200.
    1. Ley O, Deshpande C, Prapamcham B, Naghavi M. Lumped parameter thermal model for the study of vascular reactivity in the fingertip. J Biomech Eng. 2008;130(3):031012–031012.
    1. Wilson SB, Spence VA. Dynamic thermographic imaging method for quantifying dermal perfusion: potential and limitations. Med Biol Eng Comput. 1989;27(5):496–501.

Source: PubMed

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