Bactericidal and virucidal activity of ethanol and povidone-iodine

Andreas Sauerbrei, Andreas Sauerbrei

Abstract

Ethanol and povidone-iodine (PVP-I) are important microbicides that inactivate bacteria and viruses. The present study provides a review of literature data on the concentration-dependent bactericidal and virucidal activity of ethanol and PVP-I in vitro. A systematic search was performed using the meta-database for biomedicine PubMed. Eventually, 74 studies with original data on the reduction of bacterial and viral infectivity using in vitro tests were analyzed. A safe bactericidal effect of ethanol can be expected at concentrations between 60% and 85%, and the exposure times vary between ≤0.5 and ≥5 min. Within an exposure of up to 5 min, 80%-90% ethanol also exerts virucidal/low-level activity, which includes its action against enveloped viruses plus adeno-, noro-, and rotaviruses. For PVP-I, the best bactericidal and virucidal/high-level effect is present at a concentration range of approx. 0.08%-0.9% depending on the free iodine concentration. The maximum exposure times are 5 min for bacteria and 60 min for viruses. The available data may help optimize the significant inactivation of bacteria and viruses in various areas. However, as the conditions in application practice can vary, concrete recommendations for the application can only be derived to a limited extent.

Keywords: PVP-I; bactericidal/virucidal activity; ethanol; exposure time; literature data; quantitative suspension test.

Conflict of interest statement

None declared.

© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

References

    1. Adams, D. , Quayum, M. , Worthington, T. , Lambert, P. , & Elliott, T. (2005). Evaluation of a 2% chlorhexidine gluconate in 70% isopropyl alcohol skin disinfectant. Journal of Hospital Infection, 61, 287–290. 10.1016/j.jhin.2005.05.015
    1. Anagnostopoulos, A. G. , Rong, A. , Miller, D. , Tran, A. Q. , Head, T. , Lee, C. , & Lee, W. W. (2018). 0.01 hypochlorous acid as alternative skin antiseptic: An in vitro comparison. Dermatologic Surgery, 44, 1489–1493. 10.1097/DSS.0000000000001594
    1. Anderson, M. J. , Horn, M. E. , Lin, Y. C. , Parks, P. J. , & Peterson, M. L. (2010). Efficacy of concurrent application of chlorhexidine gluconate and povidone iodine against six nosocomial pathogens. American Journal of Infection Control, 38, 826–831. 10.1016/j.ajic.2010.06.022
    1. Atemnkeng, M. A. , Plaizier‐Vercammen, J. , & Schuermans, A. (2006). Comparison of free and bound iodine and iodide species as a function of the dilution of three commercial povidone‐iodine formulations and their microbicidal activity. International Journal of Pharmaceutics, 317, 161–166. 10.1016/j.ijpharm.2006.03.013
    1. Belliot, G. , Lavaux, A. , Souihel, D. , Agnello, D. , & Pothier, P. (2008). Use of murine norovirus as a surrogate to evaluate resistance of human norovirus to disinfectants. Applied and Environmental Microbiology, 74, 3315–3318. 10.1128/AEM.02148-07
    1. Berkelman, R. L. , Holland, B. W. , & Anderson, R. L. (1982). Increased bactericidal activity of dilute preparations of povidone‐iodine solution. Journal of Clinical Microbiology, 15, 635–639.
    1. Boudouma, M. , Enjalbert, I. , & Didier, J. (1984). A simple method for the evaluation of antiseptic and disinfectant virucidal activity. Journal of Virological Methods, 9, 271–276. 10.1016/0166-0934(84)90052-1
    1. Boyce, J. M. , & Pittet, D. (2002). Guideline for hand hygiene in health‐care settings: Recommendations of the healthcare infection control practices advisory committee and the HICPAC/SHEA/APIC/IDSA hand hygiene task force. Infection Control and Hospital Epidemiology, 23(12 Suppl.), S3–S40. 10.1086/503164
    1. Ciesek, S. , Friesland, M. , Steinmann, J. , Becker, B. , Wedemeyer, H. , Manns, M. P. , … Steinmann, E. (2010). How stable is hepatitis C virus (HCV)? Environmental stability of HCV and its susceptibility to chemical biocides. The Journal of Infectious Diseases, 201, 1859–1866. 10.1086/652803
    1. Doerrbecker, J. , Friesland, M. , Ciesek, S. , Erichsen, T. J. , Mateu‐Gelabert, P. , Steinmann, J. , … Steinmann, E. (2011). Inactivation and survival of hepatitis C virus on inanimate surfaces. The Journal of Infectious Diseases, 204, 1830–1838. 10.1093/infdis/jir535
    1. Doultree, J. C. , Druce, J. D. , Birch, C. J. , Bowden, D. S. , & Marshall, J. A. (1999). Inactivation of feline calicivirus, a Norwalk virus surrogate. Journal of Hospital Infection, 41, 51–57. 10.1016/s0195-6701(99)90037-3
    1. Duizer, E. , Bijkerk, P. , Rockx, B. , de Groot, A. , Twisk, F. , & Koopmans, M. (2004). Inactivation of caliciviruses. Applied and Environmental Microbiology, 70, 4538–4543. 10.1128/AEM.70.8.4538-4543.2004
    1. Eggers, M. , Eickmann, M. , Kowalski, K. , Zorn, J. , & Reimer, K. (2015). Povidone‐iodine hand wash and hand rub products demonstrated excellent in vitro virucidal efficacy against Ebola virus and modified vaccinia virus Ankara, the new European test virus for enveloped viruses. BMC Infectious Diseases, 15, 375 10.1186/s12879-015-1111-1119
    1. Eggers, M. , Eickmann, M. , & Zorn, J. (2015). Rapid and effective virucidal activity of povidone‐iodine products against Middle East Respiratory Syndrome coronavirus (MERS‐CoV) and modified vaccinia virus Ankara (MVA). Infectious Diseases and Therapy, 4, 491–501. 10.1007/s40121-015-0091-9
    1. Eggers, M. , Koburger‐Janssen, T. , Eickmann, M. , & Zorn, J. (2018). In vitro bactericidal and virucidal efficacy of povidone‐iodine gargle/mouthwash against respiratory and oral pathogens. Infectious Diseases and Therapy, 7, 249–259. 10.1007/s40121-018-0200-7
    1. EN12791:2016+A1:2017 (2017). Chemical disinfectants and antiseptics. Surgical hand disinfection. Test method and requirements (phase 2, step 2).
    1. EN14476 (2019). Chemical disinfectants and antiseptics ‐ Quantitative suspension test for the evaluation of virucidal activity in the medical area ‐ Test method and requirements (Phase 2/Step 1).
    1. EN1500 (2013). Chemical disinfectants and antiseptics ‐ Hygienic handrub ‐ Test method and requirements (phase 2/step 2).
    1. Eterpi, M. , McDonnell, G. , & Thomas, V. (2009). Disinfection efficacy against parvoviruses compared with reference viruses. Journal of Hospital Infection, 73, 64–70. 10.1016/j.jhin.2009.05.016
    1. Gehrke, C. , Steinmann, J. , & Goroncy‐Bermes, P. (2004). Inactivation of feline calicivirus, a surrogate of norovirus (formerly Norwalk‐like viruses), by different types of alcohol in vitro and in vivo. Journal of Hospital Infection, 56, 49–55. 10.1016/j.jhin.2003.08.019
    1. Ghogawala, Z. , & Furtado, D. (1990). In vitro and in vivo bactericidal activities of 10%, 2,5%, and 1% povidone‐iodine solution. American Journal of Hospital Pharmacy, 47, 1562–1566.
    1. Gold, N. A. , & Avva, U. (2020). Alcohol sanitizers. StatPearls [Internet]. Treasure Island, FL: StatPears Publishing.
    1. Goroncy‐Bermes, P. , Koburger, T. , & Meyer, B. (2010). Impact of the amount of hand rub applied in hygienic hand disinfection on the reduction of microbial counts on hands. Journal of Hospital Infection, 74, 212–218. 10.1016/j.jhin.2009.09.018
    1. Görtz, G. , Reimer, K. , & Neef, H. (1996). Eigenschaften und Bedeutung von PVP‐I In Hierholzer G., Reimer K., & Weissenbacher E. R. (Eds.), Topische Infektionstherapie und Prophylaxe (pp. 3–7). Stuttgart, Germany: Thieme.
    1. Haley, C. E. , Marling‐Cason, M. , Smith, J. W. , Luby, J. P. , & Mackowiak, P. A. (1985). Bactericidal activity of antiseptics against methicillin‐resistant Staphylococcus aureus . Journal of Clinical Microbiology, 21, 991–992.
    1. Heiner, J. D. , Hile, D. C. , Demons, S. T. , & Wedmore, I. S. (2010). 10% povidone‐iodine may be a practical field water disinfectant. Wilderness and Environmental Medicine, 21, 332–336. 10.1016/j.wem.2010.09.008
    1. Hill, R. L. , & Casewell, M. W. (2000). The in‐vitro activity of povidone‐iodine cream against Staphylococcus aureus and its bioavailability in nasal secretions. Journal of Hospital Infection, 45, 198–205. 10.1053/jhin.2000.0733
    1. Ito, H. , Ito, T. , Hikida, M. , Yashiro, J. , Otsuka, A. , Kida, H. , & Otsuki, K. (2006). Outbreak of highly pathogenic avian influenza in Japan and anti‐influenza virus activity of povidone‐iodine products. Dermatology, 212(Suppl. 1), 115–118. 10.1159/000089210
    1. Iwasawa, A. , Niwano, Y. , Kohno, M. , & Ayaki, M. (2012). Virucidal activity of alcohol‐based hand rub disinfectants. Biocontrol Science, 17, 45–49. 10.4265/bio.17.45
    1. Kampf, G. (2018). Efficacy of ethanol against viruses in hands disinfection. Journal of Hospital Infection, 98, 331–338. 10.1016/j.jhin.2017.08.025
    1. Kampf, G. , & Hollingsworth, A. (2008). Comprehensive bactericidal activity of an ethanol‐based hand gel in 15 seconds. Annals of Clinical Microbiology and Antimicrobials, 7, 2 10.1186/1476-0711-7-2
    1. Kampf, G. , & Kramer, A. (2004). Epidemiologic background of hand hygiene and evaluation of the most important agents for scrubs and rubs. Clinical Microbiology Reviews, 17, 863–893. 10.1128/CMR.17.4.863-893.2004
    1. Kampf, G. , Marschall, S. , Eggerstedt, S. , & Ostermeyer, C. (2010). Efficacy of ethanol‐based hand foams using clinically relevant amounts: A cross‐over controlled study among healthy volunteers. BMC Infectious Diseases, 10, 78 10.1186/1471-2334-10-78
    1. Kampf, G. , Rudolf, M. , Labadie, J. C. , & Barrett, S. P. (2002). Spectrum of antimicrobial activity and user acceptability of the hand disinfectant agent Sterillium Gel. Journal of Hospital Infection, 52, 141–147. 10.1053/jhin.2002.1281
    1. Kasuga, Y. , Ikenova, H. , & Okuda, K. (1997). Bactericidal effect of moth rinses on oral bacteria. The Bulletin of Tokyo Dental Collage, 38, 297–302.
    1. Kawana, R. , Kitamura, T. , Nakagomi, O. , Matsumoto, I. , Arita, M. , Yoshihara, N. , … Chiba, S. (1997). Inactivation of human viruses by povidone‐iodine in comparison with other antiseptics. Dermatology, 195(Suppl. 2), 29–35. 10.1159/000246027
    1. Kida, N. (2009). Bactericidal and sporicidal activities of an improved iodine formulation and its derivates. Biocontrol Science, 3, 113–118. 10.4265/bio.14.113
    1. Koshiro, A. , & Oie, S. (1984). Bactericidal activity of ethanol against glucose nonfermentative Gram‐negative bacilli. Microbios, 40, 33–40.
    1. Kramer, A. , Rudolph, P. , Kampf, G. , & Pittet, D. (2002). Limited efficacy of alcohol‐based hand gels. The Lancet, 359, 1489–1490. 10.1016/S0140-6736(02)08426-X
    1. Kurtz, J. B. , Lee, T. W. , & Parson, A. J. (1980). The action of alcohols on rotavirus, astrovirus and enterovirus. Journal of Hospital Infection, 1, 321–325.
    1. Magulski, T. , Paulmann, D. , Bischoff, B. , Becker, B. , Steinmann, E. , Steinmann, J. , … Steinmann, J. (2009). Inactivation of murine norovirus by chemical biocides on stainless steel. BMC Infectious Diseases, 9, 107 10.1186/1471-2334-9-107
    1. Malik, Y. S. , Meherchandani, S. , & Goyal, S. M. (2006). Comparative efficacy of ethanol and isopropanol against feline calicivirus, a norovirus surrogate. American Journal of Infection Control, 34, 31–35. 10.1016/j.ajic.2005.05.012
    1. Marchetti, M. G. , Kampf, G. , Finzi, G. , & Salvtorelli, G. (2003). Evaluation of the bactericidal effect of five products for surgical hand disinfection according to prEN 12054 and prEN 12791. Journal of Hospital Infection, 54, 63–67. 10.1016/s0195-6701(03)00039-2
    1. Matsuhira, T. , Kaij, C. , Murakami, S. , Maebashi, K. , Oka, T. , Takeda, N. , & Katayama, K. (2012). Evaluation of four antiseptics using a novel murine norovirus. Experimental Animals, 61, 35–40. 10.1538/expanim.61.35
    1. McLure, A. R. , & Gordon, J. (1992). In‐vitro evaluation of povidone‐iodine and chlorhexidine against methicillin‐resistant Staphylococcus aureus . Journal of Hospital Infection, 21, 291–299. 10.1016/0195-6701(92)90139-d
    1. Messager, S. , Goddard, P. A. , Dettmar, P. W. , & Maillard, J. Y. (2001). Determination of the antibacterial efficacy of several antiseptics tested on skin by an ‘ex‐vivo’ model. Journal of Medical Microbiology, 50, 284–292. 10.1099/0022-1317-50-3-284
    1. Møretrø, T. , Vestby, L. K. , Nesse, L. L. , Storheim, S. E. , Kotlarz, K. , & Langsrud, S. (2009). Evaluation of efficacy of disinfectants against Salmonella from the feed industry. Journal of Applied Microbiology, 106, 1005–1012. 10.1111/j.1365-2672.2008.04067.x
    1. Musumeki, R. , Bandello, F. , Martinelli, M. , Calaresu, E. , & Cocuzza, C. E. (2018). In vitro bactericidal activity of 0.6% povidone‐iodine eye drops formulation. European Journal of Ophthalmology, 201, 673–677. 10.1177/1120672118802541
    1. Nakagawa, T. , Hosaka, Y. , Ishihara, K. , Hiraishi, T. , Sato, S. , Ogawa, T. , & Kamoi, K. (2006). The efficacy of povidone‐iodine products against periodontopathic bacteria. Dermatology, 212(Suppl. 1), 109–111. 10.1159/000089208
    1. Nishioka, H. , Nagahama, A. , Inoue, Y. , & Hagi, A. (2018). Evaluation of fast‐acting bactericidal activity and substantivity of an antiseptic agent, olanexidine gluconate, using an ex vivo skin model. Journal of Medical Microbiology, 67, 1796–1803. 10.1099/jmm.0.000870
    1. Noda, N. , Watanabe, M. , Yamada, F. , & Fujimoto, S. (1981). Virucidal activity of alcohols. Virucidal efficiency of alcohols against viruses in liquid phase. Kansenshogaku Zasshi, 55, 355–366. 10.11150/kansenshogakuzasshi1970.55.355
    1. Paulmann, D. , Steinmann, J. , Becker, B. , Bischoff, B. , Steinmann, E. , & Steinmann, J. (2011). Virucidal activity of different alcohols against murine norovirus, a surrogate of human norovirus. Journal of Hospital Infection, 79, 378–382. 10.1016/j.jhin.2011.04.029
    1. Pfaender, S. , Brinkmann, J. , Todt, D. , Riebesehl, N. , Steinmann, J. , Steinmann, J. , … Steinmann, E. (2015). Mechanisms of methods for hepatitis C virus inactivation. Applied Environmental Microbiology, 81, 1616–1621. 10.1128/AEM.03580-14
    1. Rabenau, H. F. , Rapp, I. , & Steinmann, J. (2010). Can vaccinia virus be replaced by MVA virus for testing virucidal activity of chemical disinfectants? BMC Infectious Diseases, 10, 185 10.1186/1471-2334-10-185
    1. Rabenau, H. F. , Schwebke, I. , Blümel, J. , Eggers, M. , Glebe, D. , Rapp, I. , … Wutzler, P. (2014). Guideline of the German Association for the Control of Viral Diseases (DVV) eV and the Robert Koch Institute (RKI) for testing chemical disinfectants for effectiveness against viruses in human medicine. Version of 1 December, 2014. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz, 58, 493–504. 10.1007/s00103-015-2131-8
    1. Rabenau, H. F. , Schwebke, I. , Steinmann, J. , Eggers, M. , & Rapp, I. & the members of the expert committee for virus disinfection (2012). Guideline of “Deutsche Vereinigung zur Bekämpfung der Viruskrankheiten e.V.” (DVV; German Association for the Control of Virus Diseases). Quantitative test for the evaluation of virucidal activity of chemical disinfectants on non‐porous surfaces. Hygiene und Medizin, 37, 78–85.
    1. Reimer, K. , Vogt, P. M. , Broegmann, B. , Hauser, J. , Rossbach, O. , Kramer, A. , … Fleischer, W. (2000). In innovative topical drug formulation for wound healing and infection treatment: In vitro and in vivo investigations of a povidone‐iodine liposome hydrogel. Dermatology, 201, 235–241. 10.1159/000018494
    1. Reimer, K. , Wichelhaus, T. A. , Schäfer, V. , Rudolph, P. , Kramer, A. , Wutzler, P. , … Fleischer, W. (2002). Antimicrobial effectiveness of povidone‐iodine and consequences for new application areas. Dermatology, 204(Suppl. 1), 114–120. 10.1159/000057738
    1. Reybrouck, G. (1985). The bactericidal activity of aqueous disinfectants applied on living tissues. Pharmaceutisch Weekblad, 7, 100–103. 10.1007/bf01968710
    1. Rikimaru, T. , Kondo, M. , Kajimura, K. , Oyamada, K. , Miyazaki, S. , Sagawa, K. , … Oizumi, K. (2002). Efficacy of common antiseptics against multidrug‐resistant Mycobacterium tuberculosis . International Journal of Tuberculosis and Lung Disease, 6, 763–770.
    1. Rikimaru, T. , Kondo, M. , Kondo, S. , & Oizumi, K. (2000). Efficacy of common antiseptics against mycobacteria. International Journal of Tuberculosis and Lung Disease, 4, 570–576.
    1. Saknimit, M. , Inatsuki, I. , Sugiyama, Y. , & Yagami, K. (1988). Virucidal efficacy of physico‐chemical treatments against coronaviruses of laboratory animals. Experimental Animals, 37, 341–345. 10.1538/expanim1978.37.3_341
    1. Salvatico, S. , Feuillolay, C. , Mas, Y. , Verrière, F. , & Roques, C. (2015). Bactericidal activity of 3 cutaneous/mucosal antiseptic solutions in the presence of interfering substances: Improvement of the NF EN 13727 European Standard? Médecine et Maladies Infectieuses, 45, 89–94. 10.1016/j.medmal.2015.01.006
    1. Sanchez, I. R. , Nusbaum, K. E. , Swaim, S. F. , Hale, A. S. , Henderson, R. A. , & McGuire, J. A. (1988). Chlorhexidine diacetate and povidone‐iodine cytotoxicity to canine embryonic fibroblasts and Staphylococcus aureus . Veterinary Surgery, 17, 182–185. 10.1111/j.1532-950x.1988.tb00995.x
    1. Sato, J. , Miki, M. , Kubota, H. , Hitomi, J. , Tokuda, H. , Todaka‐Takai, R. , & Katayama, K. (2016). Effects of disinfectants against norovirus‐like particles predict norovirus inactivation. Microbiology and Immunology, 60, 609–616. 10.1111/1348-0421.12435
    1. Sauerbrei, A. , Eschrich, W. , Brandstädt, A. , & Wutzler, P. (2009). Sensitivity of poliovirus type 1 and ECHO virus type 1 to different groups of chemical biocides. Journal of Hospital Infection, 72, 277–279. 10.1016/j.jhin.2009.04.003
    1. Sauerbrei, A. , Schacke, M. , Glück, B. , Bust, U. , Rabenau, H. F. , & Wutzler, P. (2012). Does limited virucidal activity of biocides include duck hepatitis B virucidal action? BMC Infectious Diseases, 12, 276 10.1186/1471-2334-12-276
    1. Sauerbrei, A. , Schacke, M. , Glück, B. , Egerer, R. , & Wutzler, P. (2006). Validation of biocides against duck hepatitis B virus as a surrogate virus for the human hepatitis B virus. Journal of Hospital Infection, 64, 358–365. 10.1016/j.jhin.2006.04.013
    1. Sauerbrei, A. , Sehr, K. , Brandstädt, A. , Heim, A. , Reimer, K. , & Wutzler, P. (2004). Sensitivity of human adenoviruses to different groups of chemical biocides. Journal of Hospital Infection, 57, 59–66. 10.1016/j.jhin.2004.01.022
    1. Sauerbrei, A. , & Wutzler, P. (2010). Virucidal efficacy of PVP‐iodine‐containing disinfectants. Letters of Applied Microbiology, 51, 158–163. 10.1111/j.1472-765X.2010.02871.x
    1. Schedler, K. , Assadian, O. , Brautferger, U. , Müller, G. , Koburger, T. , Classen, S. , & Kramer, A. (2017). Proposed phase 2/ step 2 in‐vitro test on basis of EN 14561 for standardized testing of the wound antiseptics PVP‐iodine, chlorhexidine digluconate, polyhexanide and octenidine dihydrochloride. BMC Infectious Diseases, 17, 143 10.1186/s12879-017-2220-4
    1. Shimizu, M. , Okuzumi, K. , Yoneyama, A. , Kunisada, T. , Araake, M. , Ogawa, H. , & Kimura, S. (2002). In vitro antiseptic susceptibility of clinical isolates from nosocomial infections. Dermatology, 204(Suppl. 1), 21–27. 10.1159/000057720
    1. Shiraishi, T. , & Nakagawa, Y. (2002). Evaluation of the bactericidal activity of povidone‐iodine and commercially available gargle preparations. Dermatology, 204(Suppl. 1), 37–41. 10.1159/000057723
    1. Smock, E. , Demertzi, E. , Abdolrasouli, A. , Azadian, B. , & Williams, G. (2018). Antiseptic efficacy of povidone iodine and chlorhexidine gluconate skin preparation solutions used in burn surgery. Journal of Burn Care and Research, 39, 440–444. 10.1097/BCR.0000000000000621
    1. Steinmann, J. , Paulmann, D. , Becker, B. , Bischoff, B. , & Steinmann, E. (2012). Comparison of virucidal activity of alcohol‐based hand sanitizers versus antimicrobial hand soaps in vitro and in vivo . Journal of Hospital Infection, 82, 277–280. 10.1016/j.jhin.2012.08.005
    1. Suzuki, T. , Kataoka, H. , Ida, T. , Mikuniya, T. , Suzuki, T. , & Kamachi, K. (2012). Bactericidal activity of topical antiseptics and their gargles against Bordetella pertussis . Journal of Infection and Chemotherapy, 18, 272–275. 10.1007/s10156-011-0312-4
    1. Tavichakorntrakool, R. , Sungkeeree, S. , Saisud, P. , Chaiyakhot, P. , Wongwian, A. , Pakarasang, M. , … Boonsiri, P. (2014). Bactericidal efficacy of alcohol solution in community hospital and health centers. Journal of the Medical Association of Thailand, 97, S44–S48.
    1. Tyler, R. , & Ayliffe, G. A. (1987). A surface test for virucidal activity of disinfectants: Preliminary study with herpes virus. Journal of Hospital Infection, 9, 22–29. 10.1016/0195-6701(87)90090-9
    1. Tyler, R. , Ayliffe, G. A. , & Bradley, C. (1990). Virucidal activity of disinfectants: Studies with the poliovirus. Journal of Hospital Infection, 15, 339–345. 10.1016/0195-6701(90)90090-b
    1. U.S. Food and Drug Administration (2019). If soap and water are not available, hand sanitizers may be a good alternative. Retrieved from
    1. Wada, H. , Nojima, Y. , Ogawa, S. , Hayashi, N. , Sugiyama, N. , Kajiura, T. , … Yokota, K. (2016). Relationship between virucidal efficacy and free iodine concentration of povidone‐iodine in buffer solution. Biocontrol Science, 21, 21–27. 10.4265/bio.21.21
    1. Whitehaed, K. , & McCue, K. A. (2010). Virucidal efficacy of disinfectant actives against feline calicivirus, a surrogate for norovirus, in a short contact time. American Journal of Infection Control, 38, 26–30. 10.1016/j.ajic.2009.03.015
    1. Wichelhaus, T. A. , Schäfer, V. , Hunfeld, K. P. , Reimer, K. , Fleischer, W. , & Brade, V. (1998). Antibakterielle Wirksamkeit von Polyvidon‐Iod (Betaisodona®) auf hochresistente grampositive Erreger. Zentralblatt für Hygiene und Umweltmedizin, 200, 435–442.
    1. Wolff, M. H. , Schmitt, J. , Rahaus, M. , & König, A. (2001). Hepatitis A virus: A test method for virucidal activity. Journal of Hospital Infection, 6701(Suppl. A), S18–S22. 10.1016/s0195-6701(01)90007-6
    1. World Health Organization (2009). WHO guideline on hand hygiene in health care. Retrieved from
    1. World Health Organization (2020). Water, sanitation, hygiene and waste management for the COVID‐19 virus. Technical brief 3 March 2020. Retrieved from
    1. Wutzler, P. , Sauerbrei, A. , Klöcking, R. , Brögmann, B. , & Reimer, K. (2002). Virucidal activity and cytotoxicity of the liposomal formulation of povidone‐iodine. Antiviral Research, 54, 89–97. 10.1016/s0166-3542(01)00213-3
    1. Wutzler, P. , Sauerbrei, A. , Klöcking, R. , Burkhardt, J. , Schacke, M. , Thust, R. , … Reimer, R. (2000). Virucidal and chlamydicidal activities of eye drops with povidone‐iodine liposome complex. Ophthalmic Research, 32, 118–125. 10.1159/000055600
    1. Yates, K. A. , Shanks, R. M. , Kowalski, R. P. , & Romanowski, E. G. (2019). The in vitro evaluation of povidone‐iodine against multiple ocular adenoviral types. Journal of Ocular Pharmacology and Therapeutics, 35, 132–136. 10.1089/jop.2018.0122

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