Antibacterial efficacy testing of a bioelectric wound dressing against clinical wound pathogens

Hosan Kim, Inder Makin, Jeff Skiba, Amy Ho, Greggory Housler, Alexander Stojadinovic, Mina Izadjoo, Hosan Kim, Inder Makin, Jeff Skiba, Amy Ho, Greggory Housler, Alexander Stojadinovic, Mina Izadjoo

Abstract

Silver-based wound dressings have been developed for the control of bioburden in wounds. However, the popularity and extensive use of silver-based dressings has been associated with emerging microbial resistances to silver. In this study we examined in vitro antibacterial efficacy of a bioelectric dressing containing silver and zinc against various wound pathogens. Antibiotic-sensitive clinical wound isolates showed a 100% reduction in bacterial growth, except that Enterococcus faecalis isolate was shown to survive with a bacterial log10 reduction rate of less than 10(2) CFU. We also investigated antibacterial efficacy against the extended spectrum β-lactamase (ESBL) bacteria, multidrug-resistant (MDR) bacteria, and methicillin-resistant Staphylococcus aureus (MRSA). The bioelectric dressing was effective in killing wound pathogens including ESBL, MDR, and MRSA in vitro. Furthermore, based on the primary results against E. faecalis, we carried out extensive studies against several nosocomial Enterococcus species including vancomycin-resistant species. Overall, the vancomycin-sensitive or -resistant Enterococcus species were resistant to this dressing at up to 48 h, except for the vancomycin-resistant Enterococcus raffinosus isolate only showing a 100% bacterial reduction at 48 h, but not at 24 h. The results demonstrated the effective bactericidal activity of a bioelectric dressing against antibiotic-sensitive and MDR strains, but Enterococcus species are bacteriostatic.

Keywords: Antibacterial efficacy testing; bioelectric dressing; clinical wound isolates..

Figures

Fig. (1)
Fig. (1)
Antibacterial properties of the bioelectric wound dressing showing log10 reduction in CFU of bacteria after 24 h exposure against various clinical wound isolates. The numbers of the bioelectric wound dressing treated bacteria at 24 h were significantly lower when compared to the control treated at 0 hour (*P

Fig. (2)

Prolonged incubation up to 48…

Fig. (2)

Prolonged incubation up to 48 hours showing the antibacterial properties of the bioelectric…

Fig. (2)
Prolonged incubation up to 48 hours showing the antibacterial properties of the bioelectric wound dressing against Enterococcus clinical wound isolates. Data reported are the average values of three independent assays. The results are shown as means the standard error on the mean of three experiments.
Fig. (2)
Fig. (2)
Prolonged incubation up to 48 hours showing the antibacterial properties of the bioelectric wound dressing against Enterococcus clinical wound isolates. Data reported are the average values of three independent assays. The results are shown as means the standard error on the mean of three experiments.

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Source: PubMed

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