Human skin penetration of a copper tripeptide in vitro as a function of skin layer

Jurij J Hostynek, Frank Dreher, Howard I Maibach, Jurij J Hostynek, Frank Dreher, Howard I Maibach

Abstract

Objective and design: Skin retention and penetration by copper applied as glycyl-L-histidyl-L-lysine cuprate diacetate was evaluated in vitro in order to assess its potential for its transdermal delivery as an anti-inflammatory agent.

Materials and methods: Flow-through diffusion cells with 1 cm(2) exposure area were used under infinite dose conditions. 0.68% aq. copper tripeptide as permeant was applied on isolated stratum corneum, heat-separated epidermis and dermatomed skin and receptor fluid collected over 48 h in 4 h intervals using inductively coupled plasma mass spectrometry to analyze for copper in tissues and receptor fluid.

Results: The permeability coefficient of the compound through dermatomed skin was 2.43 ± 0.51 × 10(-4) cm/h; 136.2 ± 17.5 μg/cm(2) copper permeated 1 cm(2) of that tissue over 48 h, while 97 ± 6.6 μg/cm(2) were retained as depot.

Conclusions: Copper as tripeptide was delivered in potentially therapeutically effective amounts for inflammatory disease.

Figures

https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3016279/bin/11_2010_238_Figa_HTML.jpg
Chemical: Glycyl-L-histidyl-L-lysine copper cuprate, diacetate GHK-Cu(Ac)2, Formula: C18H29CuN6O8

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Source: PubMed

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