Microbiological Assessment of Moringa Oleifera Extracts and Its Incorporation in Novel Dental Remedies against Some Oral Pathogens

Hanaa Elgamily, Amani Moussa, Asmaa Elboraey, Hoda El-Sayed, Marwa Al-Moghazy, Aboelfetoh Abdalla, Hanaa Elgamily, Amani Moussa, Asmaa Elboraey, Hoda El-Sayed, Marwa Al-Moghazy, Aboelfetoh Abdalla

Abstract

Aim: To assess the antibacterial and antifungal potentials of different parts of Moringa oleifera plant using different extraction methods in attempts to formulate natural dental remedies from this plant.

Material and methods: Three solvents extracts (Ethanol, acetone, and ethyl acetate) of different parts of Egyptian Moringa tree were prepared and tested against oral pathogens: Staphylococcus aureus, Streptococcus mutans, and Candida albicans using disc diffusion method; As well as to incorporate the plant extract to formulate experimental toothpaste and mouthwash. The two dental remedies were assessed against the same microbial strains. Statistical analysis was performed using One-Way ANOVA test to compare the inhibition zone diameter and t-test.

Results: Ethanol extracts as well as leaves extracts demonstrated the highest significant mean inhibition zone values (P ≤ 0.05) against Staphylococcus aureus and Streptococcus mutans growth. However, all extracts revealed no inhibition zone against Candida albicans. For dental remedies, experimental toothpaste exhibited higher mean inhibition than the mouthwash against Staphylococcus aureus, Streptococcus mutans and only the toothpaste revealed antifungal effect against Candida albicans.

Conclusion: The different extracts of different parts of Moringa showed an antibacterial effect against Staphylococcus aureus and Streptococcus mutans growth. The novel toothpaste of ethanolic leaves extract has antimicrobial and antifungal potential effects all selected strains.

Keywords: (Novel Dental Remedies); Antimicrobial Activity; Moringa extract; Novel experimental tooth paste; mouth wash.

Figures

Figure 1
Figure 1
Antibacterial activity of ethanolic extract of Moringa Oleifera leaves against the profile growth of (a) staphylococcus aureus and (b) Streptococcus mutans
Figure 2
Figure 2
Antibacterial activity of the experimental toothpaste with the active additive Moringa leaves ethanolic extract on the profile growth of (a) staphylococcus aureus and (b) Streptococcus mutans
Figure 3
Figure 3
Antifungal activity of experimental toothpaste with the active additive Moringa leaves ethanolic extract on the profile growth of (c) Candida albicans
Figure 4
Figure 4
Mean Inhibition Zones (mm) for different parts of Moringa Oleifera plant against S. aureus growth using different extraction methods
Figure 5
Figure 5
Mean Inhibition Zones (mm) for different parts of Moringa Oleifera plant against S. mutans growth using different extraction methods
Figure 6
Figure 6
Mean Inhibition Zones (mm) for experimental mouthwash and tooth paste

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