Spatially restricted JAG1-Notch signaling in human thymus provides suitable DC developmental niches
Enrique Martín-Gayo, Sara González-García, María J García-León, Alba Murcia-Ceballos, Juan Alcain, Marina García-Peydró, Luis Allende, Belén de Andrés, María L Gaspar, María L Toribio, Enrique Martín-Gayo, Sara González-García, María J García-León, Alba Murcia-Ceballos, Juan Alcain, Marina García-Peydró, Luis Allende, Belén de Andrés, María L Gaspar, María L Toribio
Abstract
A key unsolved question regarding the developmental origin of conventional and plasmacytoid dendritic cells (cDCs and pDCs, respectively) resident in the steady-state thymus is whether early thymic progenitors (ETPs) could escape T cell fate constraints imposed normally by a Notch-inductive microenvironment and undergo DC development. By modeling DC generation in bulk and clonal cultures, we show here that Jagged1 (JAG1)-mediated Notch signaling allows human ETPs to undertake a myeloid transcriptional program, resulting in GATA2-dependent generation of CD34+ CD123+ progenitors with restricted pDC, cDC, and monocyte potential, whereas Delta-like1 signaling down-regulates GATA2 and impairs myeloid development. Progressive commitment to the DC lineage also occurs intrathymically, as myeloid-primed CD123+ monocyte/DC and common DC progenitors, equivalent to those previously identified in the bone marrow, are resident in the normal human thymus. The identification of a discrete JAG1+ thymic medullary niche enriched for DC-lineage cells expressing Notch receptors further validates the human thymus as a DC-poietic organ, which provides selective microenvironments permissive for DC development.
© 2017 Martín-Gayo et al.
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References
- Ardavin C., Wu L., Li C.L., and Shortman K.. 1993. Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population. Nature. 362:761–763. 10.1038/362761a0
- Banchereau J., and Steinman R.M.. 1998. Dendritic cells and the control of immunity. Nature. 392:245–252. 10.1038/32588
- Bell J.J., and Bhandoola A.. 2008. The earliest thymic progenitors for T cells possess myeloid lineage potential. Nature. 452:764–767. 10.1038/nature06840
- Bendriss-Vermare N., Barthélémy C., Durand I., Bruand C., Dezutter-Dambuyant C., Moulian N., Berrih-Aknin S., Caux C., Trinchieri G., and Brière F.. 2001. Human thymus contains IFN-alpha-producing CD11c(-), myeloid CD11c(+), and mature interdigitating dendritic cells. J. Clin. Invest. 107:835–844. 10.1172/JCI11734
- Blom B., and Spits H.. 2006. Development of human lymphoid cells. Annu. Rev. Immunol. 24:287–320. 10.1146/annurev.immunol.24.021605.090612
- Breton G., Lee J., Zhou Y.J., Schreiber J.J., Keler T., Puhr S., Anandasabapathy N., Schlesinger S., Caskey M., Liu K., and Nussenzweig M.C.. 2015a Circulating precursors of human CD1c+ and CD141+ dendritic cells. J. Exp. Med. 212:401–413. 10.1084/jem.20141441
- Breton G., Lee J., Liu K., and Nussenzweig M.C.. 2015b Defining human dendritic cell progenitors by multiparametric flow cytometry. Nat. Protoc. 10:1407–1422. 10.1038/nprot.2015.092
- Brocker T., Riedinger M., and Karjalainen K.. 1997. Targeted expression of major histocompatibility complex (MHC) class II molecules demonstrates that dendritic cells can induce negative but not positive selection of thymocytes in vivo. J. Exp. Med. 185:541–550. 10.1084/jem.185.3.541
- Cheng P., Nefedova Y., Corzo C.A., and Gabrilovich D.I.. 2007. Regulation of dendritic-cell differentiation by bone marrow stroma via different Notch ligands. Blood. 109:507–515. 10.1182/blood-2006-05-025601
- del Hoyo G.M., Martín P., Vargas H.H., Ruiz S., Arias C.F., and Ardavín C.. 2002. Characterization of a common precursor population for dendritic cells. Nature. 415:1043–1047. 10.1038/4151043a
- de Pooter R.F., Schmitt T.M., de la Pompa J.L., Fujiwara Y., Orkin S.H., and Zúñiga-Pflücker J.C.. 2006. Notch signaling requires GATA-2 to inhibit myelopoiesis from embryonic stem cells and primary hemopoietic progenitors. J. Immunol. 176:5267–5275. 10.4049/jimmunol.176.9.5267
- De Smedt M., Hoebeke I., Reynvoet K., Leclercq G., and Plum J.. 2005. Different thresholds of Notch signaling bias human precursor cells toward B-, NK-, monocytic/dendritic-, or T-cell lineage in thymus microenvironment. Blood. 106:3498–3506. 10.1182/blood-2005-02-0496
- de Yébenes V.G., Carrasco Y.R., Ramiro A.R., and Toribio M.L.. 2002. Identification of a myeloid intrathymic pathway of dendritic cell development marked by expression of the granulocyte macrophage-colony-stimulating factor receptor. Blood. 99:2948–2956. 10.1182/blood.V99.8.2948
- Dontje W., Schotte R., Cupedo T., Nagasawa M., Scheeren F., Gimeno R., Spits H., and Blom B.. 2006. Delta-like1-induced Notch1 signaling regulates the human plasmacytoid dendritic cell versus T-cell lineage decision through control of GATA-3 and Spi-B. Blood. 107:2446–2452. 10.1182/blood-2005-05-2090
- Doulatov S., Notta F., Eppert K., Nguyen L.T., Ohashi P.S., and Dick J.E.. 2010. Revised map of the human progenitor hierarchy shows the origin of macrophages and dendritic cells in early lymphoid development. Nat. Immunol. 11:585–593. 10.1038/ni.1889
- Feyerabend T.B., Terszowski G., Tietz A., Blum C., Luche H., Gossler A., Gale N.W., Radtke F., Fehling H.J., and Rodewald H.R.. 2009. Deletion of Notch1 converts pro-T cells to dendritic cells and promotes thymic B cells by cell-extrinsic and cell-intrinsic mechanisms. Immunity. 30:67–79. 10.1016/j.immuni.2008.10.016
- Fogg D.K., Sibon C., Miled C., Jung S., Aucouturier P., Littman D.R., Cumano A., and Geissmann F.. 2006. A clonogenic bone marrow progenitor specific for macrophages and dendritic cells. Science. 311:83–87. 10.1126/science.1117729
- Gao E.K., Lo D., and Sprent J.. 1990. Strong T cell tolerance in parent----F1 bone marrow chimeras prepared with supralethal irradiation. Evidence for clonal deletion and anergy. J. Exp. Med. 171:1101–1121. 10.1084/jem.171.4.1101
- García-Peydró M., de Yébenes V.G., and Toribio M.L.. 2006. Notch1 and IL-7 receptor interplay maintains proliferation of human thymic progenitors while suppressing non-T cell fates. J. Immunol. 177:3711–3720. 10.4049/jimmunol.177.6.3711
- Geissmann F., Manz M.G., Jung S., Sieweke M.H., Merad M., and Ley K.. 2010. Development of monocytes, macrophages, and dendritic cells. Science. 327:656–661. 10.1126/science.1178331
- González-García S., García-Peydró M., Martín-Gayo E., Ballestar E., Esteller M., Bornstein R., de la Pompa J.L., Ferrando A.A., and Toribio M.L.. 2009. CSL-MAML-dependent Notch1 signaling controls T lineage-specific IL-7Rα gene expression in early human thymopoiesis and leukemia. J. Exp. Med. 206:779–791. 10.1084/jem.20081922
- Haniffa M., Collin M., and Ginhoux F.. 2013. Ontogeny and functional specialization of dendritic cells in human and mouse. Adv. Immunol. 120:1–49. 10.1016/B978-0-12-417028-5.00001-6
- Hao Q.L., George A.A., Zhu J., Barsky L., Zielinska E., Wang X., Price M., Ge S., and Crooks G.M.. 2008. Human intrathymic lineage commitment is marked by differential CD7 expression: identification of CD7- lympho-myeloid thymic progenitors. Blood. 111:1318–1326. 10.1182/blood-2007-08-106294
- Hozumi K., Mailhos C., Negishi N., Hirano K., Yahata T., Ando K., Zuklys S., Holländer G.A., Shima D.T., and Habu S.. 2008. Delta-like 4 is indispensable in thymic environment specific for T cell development. J. Exp. Med. 205:2507–2513. 10.1084/jem.20080134
- Jaleco A.C., Neves H., Hooijberg E., Gameiro P., Clode N., Haury M., Henrique D., and Parreira L.. 2001. Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation. J. Exp. Med. 194:991–1002. 10.1084/jem.194.7.991
- Koch U., Fiorini E., Benedito R., Besseyrias V., Schuster-Gossler K., Pierres M., Manley N.R., Duarte A., Macdonald H.R., and Radtke F.. 2008. Delta-like 4 is the essential, nonredundant ligand for Notch1 during thymic T cell lineage commitment. J. Exp. Med. 205:2515–2523. 10.1084/jem.20080829
- Krueger A. 2011. A missing link in thymic dendritic cell development. Eur. J. Immunol. 41:2145–2147. 10.1002/eji.201141850
- Kurobe H., Liu C., Ueno T., Saito F., Ohigashi I., Seach N., Arakaki R., Hayashi Y., Kitagawa T., Lipp M., et al. . 2006. CCR7-dependent cortex-to-medulla migration of positively selected thymocytes is essential for establishing central tolerance. Immunity. 24:165–177. 10.1016/j.immuni.2005.12.011
- Lee J., Breton G., Oliveira T.Y., Zhou Y.J., Aljoufi A., Puhr S., Cameron M.J., Sékaly R.P., Nussenzweig M.C., and Liu K.. 2015. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J. Exp. Med. 212:385–399. 10.1084/jem.20141442
- Lehar S.M., Dooley J., Farr A.G., and Bevan M.J.. 2005. Notch ligands Delta 1 and Jagged1 transmit distinct signals to T-cell precursors. Blood. 105:1440–1447. 10.1182/blood-2004-08-3257
- Li J., Park J., Foss D., and Goldschneider I.. 2009. Thymus-homing peripheral dendritic cells constitute two of the three major subsets of dendritic cells in the steady-state thymus. J. Exp. Med. 206:607–622. 10.1084/jem.20082232
- Liu K., and Nussenzweig M.C.. 2010. Origin and development of dendritic cells. Immunol. Rev. 234:45–54. 10.1111/j.0105-2896.2009.00879.x
- Liu K., Victora G.D., Schwickert T.A., Guermonprez P., Meredith M.M., Yao K., Chu F.F., Randolph G.J., Rudensky A.Y., and Nussenzweig M.. 2009. In vivo analysis of dendritic cell development and homeostasis. Science. 324:392–397.
- Luche H., Ardouin L., Teo P., See P., Henri S., Merad M., Ginhoux F., and Malissen B.. 2011. The earliest intrathymic precursors of CD8α(+) thymic dendritic cells correspond to myeloid-type double-negative 1c cells. Eur. J. Immunol. 41:2165–2175. 10.1002/eji.201141728
- Márquez C., Trigueros C., Fernández E., and Toribio M.L.. 1995. The development of T and non-T cell lineages from CD34+ human thymic precursors can be traced by the differential expression of CD44. J. Exp. Med. 181:475–483. 10.1084/jem.181.2.475
- Martín-Gayo E., Sierra-Filardi E., Corbí A.L., and Toribio M.L.. 2010. Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development. Blood. 115:5366–5375. 10.1182/blood-2009-10-248260
- Merad M., Sathe P., Helft J., Miller J., and Mortha A.. 2013. The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting. Annu. Rev. Immunol. 31:563–604. 10.1146/annurev-immunol-020711-074950
- Miller J.C., Brown B.D., Shay T., Gautier E.L., Jojic V., Cohain A., Pandey G., Leboeuf M., Elpek K.G., Helft J., et al. Immunological Genome Consortium . 2012. Deciphering the transcriptional network of the dendritic cell lineage. Nat. Immunol. 13:888–899. 10.1038/ni.2370
- Mohtashami M., Shah D.K., Nakase H., Kianizad K., Petrie H.T., and Zúñiga-Pflücker J.C.. 2010. Direct comparison of Dll1- and Dll4-mediated Notch activation levels shows differential lymphomyeloid lineage commitment outcomes. J. Immunol. 185:867–876. 10.4049/jimmunol.1000782
- Moore A.J., Sarmiento J., Mohtashami M., Braunstein M., Zúñiga-Pflücker J.C., and Anderson M.K.. 2012. Transcriptional priming of intrathymic precursors for dendritic cell development. Development. 139:373–384. 10.1242/dev.069344
- Naik S.H., Sathe P., Park H.Y., Metcalf D., Proietto A.I., Dakic A., Carotta S., O’Keeffe M., Bahlo M., Papenfuss A., et al. . 2007. Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo. Nat. Immunol. 8:1217–1226. 10.1038/ni1522
- Neves H., Weerkamp F., Gomes A.C., Naber B.A.E., Gameiro P., Becker J.D., Lúcio P., Clode N., van Dongen J.J.M., Staal F.J.T., and Parreira L.. 2006. Effects of Delta1 and Jagged1 on early human hematopoiesis: correlation with expression of notch signaling-related genes in CD34+ cells. Stem Cells. 24:1328–1337. 10.1634/stemcells.2005-0207
- Okada T., Lian Z.X., Naiki M., Ansari A.A., Ikehara S., and Gershwin M.E.. 2003. Murine thymic plasmacytoid dendritic cells. Eur. J. Immunol. 33:1012–1019. 10.1002/eji.200323616
- Onai N., Obata-Onai A., Schmid M.A., Ohteki T., Jarrossay D., and Manz M.G.. 2007. Identification of clonogenic common Flt3+M-CSFR+ plasmacytoid and conventional dendritic cell progenitors in mouse bone marrow. Nat. Immunol. 8:1207–1216. 10.1038/ni1518
- Onodera K., Fujiwara T., Onishi Y., Itoh-Nakadai A., Okitsu Y., Fukuhara N., Ishizawa K., Shimizu R., Yamamoto M., and Harigae H.. 2016. GATA2 regulates dendritic cell differentiation. Blood. 128:508–518. 10.1182/blood-2016-02-698118
- Petrie H.T., and Zúñiga-Pflücker J.C.. 2007. Zoned out: functional mapping of stromal signaling microenvironments in the thymus. Annu. Rev. Immunol. 25:649–679. 10.1146/annurev.immunol.23.021704.115715
- Pui J.C., Allman D., Xu L., DeRocco S., Karnell F.G., Bakkour S., Lee J.Y., Kadesch T., Hardy R.R., Aster J.C., and Pear W.S.. 1999. Notch1 expression in early lymphopoiesis influences B versus T lineage determination. Immunity. 11:299–308. 10.1016/S1074-7613(00)80105-3
- Radtke F., Wilson A., Stark G., Bauer M., van Meerwijk J., MacDonald H.R., and Aguet M.. 1999. Deficient T cell fate specification in mice with an induced inactivation of Notch1. Immunity. 10:547–558. 10.1016/S1074-7613(00)80054-0
- Radtke F., Ferrero I., Wilson A., Lees R., Aguet M., and MacDonald H.R.. 2000. Notch1 deficiency dissociates the intrathymic development of dendritic cells and T cells. J. Exp. Med. 191:1085–1094. 10.1084/jem.191.7.1085
- Reizis B., Bunin A., Ghosh H.S., Lewis K.L., and Sisirak V.. 2011. Plasmacytoid dendritic cells: recent progress and open questions. Annu. Rev. Immunol. 29:163–183. 10.1146/annurev-immunol-031210-101345
- Robert-Moreno A., Espinosa L., de la Pompa J.L., and Bigas A.. 2005. RBPjkappa-dependent Notch function regulates Gata2 and is essential for the formation of intra-embryonic hematopoietic cells. Development. 132:1117–1126. 10.1242/dev.01660
- Rodrigues N.P., Boyd A.S., Fugazza C., May G.E., Guo Y., Tipping A.J., Scadden D.T., Vyas P., and Enver T.. 2008. GATA-2 regulates granulocyte-macrophage progenitor cell function. Blood. 112:4862–4873. 10.1182/blood-2008-01-136564
- Satpathy A.T., Wu X., Albring J.C., and Murphy K.M.. 2012. Re(de)fining the dendritic cell lineage. Nat. Immunol. 13:1145–1154. 10.1038/ni.2467
- Schlenner S.M., and Rodewald H.R.. 2010. Early T cell development and the pitfalls of potential. Trends Immunol. 31:303–310. 10.1016/j.it.2010.06.002
- Schmitt T.M., and Zúñiga-Pflücker J.C.. 2002. Induction of T cell development from hematopoietic progenitor cells by delta-like-1 in vitro. Immunity. 17:749–756. 10.1016/S1074-7613(02)00474-0
- Schmitt T.M., Ciofani M., Petrie H.T., and Zúñiga-Pflücker J.C.. 2004. Maintenance of T cell specification and differentiation requires recurrent notch receptor-ligand interactions. J. Exp. Med. 200:469–479. 10.1084/jem.20040394
- Schotte R., Rissoan M.C., Bendriss-Vermare N., Bridon J.M., Duhen T., Weijer K., Brière F., and Spits H.. 2003. The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development. Blood. 101:1015–1023. 10.1182/blood-2002-02-0438
- Shortman K., and Liu Y.J.. 2002. Mouse and human dendritic cell subtypes. Nat. Rev. Immunol. 2:151–161. 10.1038/nri746
- Shortman K., and Wu L.. 1996. Early T lymphocyte progenitors. Annu. Rev. Immunol. 14:29–47. 10.1146/annurev.immunol.14.1.29
- Six E.M., Bonhomme D., Monteiro M., Beldjord K., Jurkowska M., Cordier-Garcia C., Garrigue A., Dal Cortivo L., Rocha B., Fischer A., et al. . 2007. A human postnatal lymphoid progenitor capable of circulating and seeding the thymus. J. Exp. Med. 204:3085–3093. 10.1084/jem.20071003
- Steinman R.M. 2012. Decisions about dendritic cells: past, present, and future. Annu. Rev. Immunol. 30:1–22. 10.1146/annurev-immunol-100311-102839
- Strobl H., Scheinecker C., Riedl E., Csmarits B., Bello-Fernandez C., Pickl W.F., Majdic O., and Knapp W.. 1998. Identification of CD68+lin- peripheral blood cells with dendritic precursor characteristics. J. Immunol. 161:740–748.
- Vandenabeele S., Hochrein H., Mavaddat N., Winkel K., and Shortman K.. 2001. Human thymus contains 2 distinct dendritic cell populations. Blood. 97:1733–1741. 10.1182/blood.V97.6.1733
- Van de Walle I., De Smet G., Gärtner M., De Smedt M., Waegemans E., Vandekerckhove B., Leclercq G., Plum J., Aster J.C., Bernstein I.D., et al. . 2011. Jagged2 acts as a Delta-like Notch ligand during early hematopoietic cell fate decisions. Blood. 117:4449–4459. 10.1182/blood-2010-06-290049
- Van de Walle I., Waegemans E., De Medts J., De Smet G., De Smedt M., Snauwaert S., Vandekerckhove B., Kerre T., Leclercq G., Plum J., et al. . 2013. Specific Notch receptor-ligand interactions control human TCR-αβ/γδ development by inducing differential Notch signal strength. J. Exp. Med. 210:683–697. 10.1084/jem.20121798
- Van de Walle I., Dolens A.C., Durinck K., De Mulder K., Van Loocke W., Damle S., Waegemans E., De Medts J., Velghe I., De Smedt M., et al. . 2016. GATA3 induces human T-cell commitment by restraining Notch activity and repressing NK-cell fate. Nat. Commun. 7:11171–11185. 10.1038/ncomms11171
- van Rijn R.S., Simonetti E.R., Hagenbeek A., Hogenes M.C., de Weger R.A., Canninga-van Dijk M.R., Weijer K., Spits H., Storm G., van Bloois L., et al. . 2003. A new xenograft model for graft-versus-host disease by intravenous transfer of human peripheral blood mononuclear cells in RAG2-/- gammac-/- double-mutant mice. Blood. 102:2522–2531. 10.1182/blood-2002-10-3241
- von Boehmer H. 2009. Notch 1 keeps pro-T cells on track. Immunity. 30:5–7. 10.1016/j.immuni.2008.12.008
- Wada H., Masuda K., Satoh R., Kakugawa K., Ikawa T., Katsura Y., and Kawamoto H.. 2008. Adult T-cell progenitors retain myeloid potential. Nature. 452:768–772. 10.1038/nature06839
- Watanabe N., Wang Y.H., Lee H.K., Ito T., Wang Y.H., Cao W., and Liu Y.J.. 2005. Hassall’s corpuscles instruct dendritic cells to induce CD4+CD25+ regulatory T cells in human thymus. Nature. 436:1181–1185. 10.1038/nature03886
- Weijer K., Uittenbogaart C.H., Voordouw A., Couwenberg F., Seppen J., Blom B., Vyth-Dreese F.A., and Spits H.. 2002. Intrathymic and extrathymic development of human plasmacytoid dendritic cell precursors in vivo. Blood. 99:2752–2759. 10.1182/blood.V99.8.2752
- Wilson A., MacDonald H.R., and Radtke F.. 2001. Notch 1-deficient common lymphoid precursors adopt a B cell fate in the thymus. J. Exp. Med. 194:1003–1012. 10.1084/jem.194.7.1003
- Wu L., and Shortman K.. 2005. Heterogeneity of thymic dendritic cells. Semin. Immunol. 17:304–312. 10.1016/j.smim.2005.05.001
- Yui M.A., and Rothenberg E.V.. 2014. Developmental gene networks: a triathlon on the course to T cell identity. Nat. Rev. Immunol. 14:529–545. 10.1038/nri3702
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