Relationship between pregnancy, embryo development, and sperm deoxyribonucleic acid fragmentation dynamics

Artur Wdowiak, Iwona Bojar, Artur Wdowiak, Iwona Bojar

Abstract

The way the dynamics of DNA fragmentation affects the growth of embryos in real time, and effectiveness of infertility treatment using the ICSI procedure were determined in 148 couples treated with the ICSI technique. The percentage of sperm with fragmented DNA (known as the DNA fragmentation index [DFI]) in semen samples was determined at 3, 6 and 12 h. Embryo culture was assessed continuously during 12 h of observation monitoring. Statistically significant difference was found in DFI at 12 h and outcome of treatment. For the remaining time intervals, no statistically significant differences were noted. An analysis of relationship between the DFI dynamics over time at individual measurements and achievement of pregnancy, confirmed a statistically significant relationship between the rate measured at 6-12 h of observations of DFI changes (DFI 12 h%/h), and achieving pregnancy. Correlation was observed between DFI (during 0, 3, 6 and 12 h), the growth rate in DFI, and time of embryo development. A statistically significant relationship was found between the rate from the start to the end of observations of the DFI, and outcome of treatment. Intensity level regarding fragmentation of sperm DNA and its growth rate affected the time of embryo development in the ICSI procedure. The most significant prognostic factor for achieving pregnancy was intensification of sperm DNA fragmentation after 12 h.

Keywords: Infertility; Intracytoplasmic sperm injection; Sperm DNA fragmentation dynamics; Time-lapse embryo.

Figures

Figure 1
Figure 1
DFI after 12 h in groups with and without achieved pregnancy, Z = −3.499, p = 0.0005.
Figure 2
Figure 2
Rate of DFI growth rate in individual measurement times (xh%/h) in groups where pregnancy was achieved and where pregnancy did not occur.
Figure 3
Figure 3
Dynamics of development of an embryo at individual measurement times (t) in groups where pregnancy was achieved and where pregnancy did not occur.

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Source: PubMed

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