Influence of human serum on pharmacodynamic properties of an investigational glycopeptide, LY333328, and comparator agents against Staphylococcus aureus
G G Zhanel, I D Kirkpatrick, D J Hoban, A M Kabani, J A Karlowsky, G G Zhanel, I D Kirkpatrick, D J Hoban, A M Kabani, J A Karlowsky
Abstract
The MICs and MBCs of 15 antibiotics for two strains of Staphylococcus aureus were determined in Mueller-Hinton broth (MHB) and 90% serum-10% MHB. Subsequent experiments established that highly protein-bound antibiotics (>/=80%), such as LY333328, demonstrated higher MICs and MBCs, less killing over an 8-h interval, and shorter postantibiotic effects in 90% serum-10% MHB than in MHB alone. Albumin was demonstrated to be almost solely responsible for changes in the aforementioned pharmacodynamic parameters of LY333328.
Figures
Representative time-kill curve of ATCC 29213 S. aureus exposed to LY333328 at a concentration equal to the MIC in MHB (0.5 μg/ml). Symbols: ■, MHB control; ▴, MHB plus LY333328; •, MHBA control; ⧫, MHBA plus LY333328; ★, MHBS control; ✚, MHBS plus LY333328.
Source: PubMed