In vivo comparison of cavity disinfection efficacy with APF gel, Propolis, Diode Laser, and 2% chlorhexidine in primary teeth

P V M Uday Mohan, K S Uloopi, C Vinay, R Chandrasekhar Rao, P V M Uday Mohan, K S Uloopi, C Vinay, R Chandrasekhar Rao

Abstract

Background: The survival of atraumatic restorative treatment (ART) restorations would be enhanced if near total elimination of cariogenic microorganisms could be done in the process of cavity cleaning before placing a restoration. Thus, use of disinfecting agents for achieving this goal could herald a new beginning in the field of contemporary dentistry.

Aim: To assess and compare the cavity disinfection efficacy of APF gel, Brazilian Propolis, Diode Laser, and 2% chlorhexidine (CHX).

Materials and methods: The study was a randomized, single blinded, parallel grouped, active controlled trial. Eighty primary molars in 68 children with cavitated dentinal occlusal caries were randomly assigned into four groups (20 teeth each) Group I: APF gel; Group II: Propolis; Group III: Diode Laser, and Group IV: 2% CHX (control). After cavity preparation using ART procedure, dentinal samples collected before and after disinfection with respective agent of the group. These samples were subjected to microbiological evaluation, for total viable count (TVC) on blood agar, Streptococcus mutans on mutans-sanguis (MS) agar, and Lactobacilli (LB) on Rogosa agar.

Results: Intragroup comparison (Wilcoxon signed rank test) showed significant reductions in TVC, MS, and LB counts in all the groups. Pairwise Mann-Whitney test showed APF gel had least bacterial reductions among the agents tested.

Conclusion: This study illustrated the need for cavity disinfection. Diode Laser and Brazilian Propolis are equally effective as 2% CHX in cavity disinfection.

Keywords: APF gel; Propolis; atraumatic restorative treatment; cavity disinfection; diode Laser and 2% chlorhexidine.

Figures

Figure 1
Figure 1
Summary flow chart of study design
Figure 2
Figure 2
(A) Total viable count before cavity disinfection in Group I; (B) total viable count after cavity disinfection in Group I; (C) mutans sanguis counts before cavity disinfection in Group I; (D) mutans sanguis counts after cavity disinfection in Group I; (E) Lactobacilli counts before cavity disinfection in Group I; and (F) Lactobacilli counts after cavity disinfection in Group I
Figure 3
Figure 3
(A) Total viable count before cavity disinfection in Group II; (B) total viable count after cavity disinfection in Group II; (C) mutans sanguis counts before cavity disinfection in Group II; (D) mutans sanguis counts after cavity disinfection in Group II; (E) Lactobacilli counts before cavity disinfection in Group II; and (F) Lactobacilli counts after cavity disinfection in Group II
Figure 4
Figure 4
(A) Total viable count before cavity disinfection in Group III; (B) total viable count after cavity disinfection in Group III; (C) mutans sanguis counts before cavity disinfection in Group III; (D) mutans sanguis counts after cavity disinfection in Group III; (E) Lactobacilli counts before cavity disinfection in Group III; and (F) Lactobacilli counts after cavity disinfection in Group III
Figure 5
Figure 5
(A) Total viable count before cavity disinfection in Group IV; (B) total viable count after cavity disinfection in Group IV; (C) mutans sanguis counts before cavity disinfection in Group IV; (D) mutans sanguis counts after cavity disinfection in Group IV; (E) Lactobacilli counts before cavity disinfection in Group IV; and (F) Lactobacilli counts after cavity disinfection in Group IV

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Source: PubMed

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