The effect of glucocorticosteroids on in vitro motility of the ureter of the sheep
M Angelo-Khattar, O Thulesius, T Cherian, M Angelo-Khattar, O Thulesius, T Cherian
Abstract
1. The effects of three glucocorticosteroids, hydrocortisone, methylprednisolone and dexamethasone, as well as two non-steroidal anti-inflammatory agents (NSAIDs) indomethacin and diclofenac sodium were tested in vitro on the spontaneously rhythmic contracting ureteral preparation of the sheep. 2. The NSAIDs and the steroids methylprednisolone (10(-7)-10(-4) M) and dexamethasone (10(-8) - 10(-4) M), dose-dependently inhibited ureteral motility. Hydrocortisone caused a cessation of spontaneous contractions only at the high concentration of 10(-4) M. 3. Pretreatment of ureteral strips with the protein synthesis inhibitor cycloheximide (10(-6) M) abolished the inhibitory action of the corticosteroids on peristalsis, consistent with the suggestion that the action of steroids on the ureter is mediated via the synthesis of the anti-phospholipase protein, lipocortin. 4. The potency of the steroids in descending order was found to be dexamethasone greater than methylprednisolone greater than hydrocortisone.
References
- Proc Natl Acad Sci U S A. 1976 May;73(5):1730-4
- Urol Res. 1988;16(4):287-93
- Nature. 1979 Mar 29;278(5703):456-9
- Proc Natl Acad Sci U S A. 1979 Jun;76(6):2640-3
- Nature. 1980 Sep 11;287(5778):147-9
- Lancet. 1982 May 15;1(8281):1096-7
- Biochim Biophys Acta. 1982 Jul 20;712(1):177-85
- Br J Urol. 1982 Oct;54(5):446-50
- Agents Actions. 1984 Jan;14(1):21-30
- Acta Pharmacol Toxicol (Copenh). 1985 Apr;56(4):298-301
- Acta Physiol Scand. 1986 Aug;127(4):485-90
- Scand J Urol Nephrol. 1986;20(3):225-9
- Gen Pharmacol. 1987;18(3):337-40
- Acta Physiol Scand. 1987 Sep;131(1):51-4
- J Urol. 1978 Dec;120(6):676-7
Source: PubMed