Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19
Robin McKenzie, Chad K Porter, Joyce A Cantrell, Barbara Denearing, Aisling O'Dowd, Shannon L Grahek, Stephanie A Sincock, Colleen Woods, Peter Sebeny, David A Sack, David R Tribble, A Louis Bourgeois, Stephen J Savarino, Robin McKenzie, Chad K Porter, Joyce A Cantrell, Barbara Denearing, Aisling O'Dowd, Shannon L Grahek, Stephanie A Sincock, Colleen Woods, Peter Sebeny, David A Sack, David R Tribble, A Louis Bourgeois, Stephen J Savarino
Abstract
Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 10(9) organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 10(9) organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.
Source: PubMed