A reach-out system for video microscopy analysis of ciliary motions aiding PCD diagnosis

Israel Amirav, Huda Mussaffi, Yehudah Roth, Miriam Schmidts, Heymut Omran, Claudius Werner, Israeli PCD Consortium Investigators, Revital Abitbul, Soliman Alkrinawi, Micha Aviram, Avi Avital, Dorit Baso, Lea Bentur, Hannah Blau, Malena Cohen-Cymberknoh, Husein Dabbah, Nael Elias, Arnon Elizur, Shmuel Goldberg, Avigdor Hevroni, Eytan Kerem, Moran Lavie, Galit Livnat, Anthony Luder, Avigdor Mandelberg, Michael Paz, Elie Picard, Joseph Rivlin, Moshe Rotschild, David Shoseyov, Ruth Soferman, Chaim Springer, Johanna Raidt, Israel Amirav, Huda Mussaffi, Yehudah Roth, Miriam Schmidts, Heymut Omran, Claudius Werner, Israeli PCD Consortium Investigators, Revital Abitbul, Soliman Alkrinawi, Micha Aviram, Avi Avital, Dorit Baso, Lea Bentur, Hannah Blau, Malena Cohen-Cymberknoh, Husein Dabbah, Nael Elias, Arnon Elizur, Shmuel Goldberg, Avigdor Hevroni, Eytan Kerem, Moran Lavie, Galit Livnat, Anthony Luder, Avigdor Mandelberg, Michael Paz, Elie Picard, Joseph Rivlin, Moshe Rotschild, David Shoseyov, Ruth Soferman, Chaim Springer, Johanna Raidt

Abstract

Backgrounds: High-speed Video-Microscopy Analysis (HVMA) is now being used to aid diagnosis of Primary Ciliary Dyskinesia (PCD). Only a few centers however, are equipped with the available resources and equipment to perform these tests. We describe our experience in HVMA reaching-out to many more peripheral and relatively remote areas. A portable computer with HVMA software, video camera and a microscope were used. Fourteen disperse pediatric centers were reached and a total of 203 subjects were tested within a relatively short time (Clinical Trial Registration: NCT 01070914 (registered February 6, 2010).

Results: With an average time of 20 minutes per patient, the system enabled us to test approximately 10-15 subjects per day. A valid HVMA result was made in 148 subjects and helped in the diagnosis of PCD in many of the patients who were subsequently confirmed to have PCD by electron microscopy and/or immunofluoresence and/or genetics and/or nasal Nitric Oxide testing. The sensitivity of abnormal HVMA to accurately predict PCD was 90.2%.

Discussion and conclusion: This is the first report of an out-reach system to record HVMA for improved diagnosis of PCD in remote regions that are not within reach of PCD centers and experts. It provides immediate preliminary results and instantaneous feedback to the physician, patient and his/her family members in these areas. Future studies to compare this system to conventional desk top systems are warranted.

Trial registration: NCT 01070914 (registered February 6, 2010).

Trial registration: ClinicalTrials.gov NCT01070914.

Figures

Figure 1
Figure 1
Use of a 24 well plate for microscopy.
Figure 2
Figure 2
Components of the HVMA system.
Figure 3
Figure 3
HVMA on-site results (percentage of each category) in all subjects (n = 203) (CBA = cannot be assessed).
Figure 4
Figure 4
HVMA post-hoc results (percentage of each category) in PCD patients (n = 112).

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Source: PubMed

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