Prospective study into the value of the automated Elecsys antimüllerian hormone assay for the assessment of the ovarian growing follicle pool
Richard A Anderson, Ellen Anckaert, Ernesto Bosch, Didier Dewailly, Cheryl E Dunlop, Daniel Fehr, Luciano Nardo, Johan Smitz, Kelton Tremellen, Barbara Denk, Andrea Geistanger, Martin Hund, Richard A Anderson, Ellen Anckaert, Ernesto Bosch, Didier Dewailly, Cheryl E Dunlop, Daniel Fehr, Luciano Nardo, Johan Smitz, Kelton Tremellen, Barbara Denk, Andrea Geistanger, Martin Hund
Abstract
Objective: To evaluate a new fully automated assay measuring antimüllerian hormone (AMH; Roche Elecsys) against antral follicle count in women of reproductive age.
Design: Prospective cohort study.
Setting: Hospital infertility clinics and academic centers.
Patient(s): Four hundred fifty-one women aged 18 to 44 years, with regular menstrual cycles.
Intervention(s): None.
Main outcome measure(s): AMH and antral follicle count (AFC) determined at a single visit on day 2-4 of the menstrual cycle.
Result(s): There was a statistically significant variance in AFC but not in AMH between centers. Both AFC and AMH varied by age (overall Spearman rho -0.50 for AFC and -0.47 for AMH), but there was also significant between-center variation in the relationship between AFC and age but not for AMH. There was a strong positive correlation between AMH and AFC (overall spearman rho 0.68), which varied from 0.49 to 0.87 between centers. An agreement table using AFC cutoffs of 7 and 15 showed classification agreement in 63.2%, 56.9% and 74.5% of women for low, medium, and high groups, respectively.
Conclusion(s): The novel fully automated Elecsys AMH assay shows good correlations with age and AFC in women of reproductive age, providing a reproducible measure of the growing follicle pool.
Keywords: Antimüllerian hormone; antral follicle count; ovarian follicle; ovarian reserve; reproductive life span.
Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
Source: PubMed