Antibacterial properties of tualang honey and its effect in burn wound management: a comparative study

Nur-Azida Mohd Nasir, Ahmad Sukari Halim, Kirnpal-Kaur Banga Singh, Ananda Aravazhi Dorai, Mehru-Nisha Muhammad Haneef, Nur-Azida Mohd Nasir, Ahmad Sukari Halim, Kirnpal-Kaur Banga Singh, Ananda Aravazhi Dorai, Mehru-Nisha Muhammad Haneef

Abstract

Background: The use of honey as a natural product of Apis spp. for burn treatment has been widely applied for centuries. Tualang honey has been reported to have antibacterial properties against various microorganisms, including those from burn-related diagnoses, and is cheaper and easier to be absorbed by Aquacel dressing. The aim of this study is to evaluate the potential antibacterial properties of tualang honey dressing and to determine its effectiveness as a partial thickness burn wound dressing.

Methods: In order to quantitate the bioburden of the swabs, pour plates were performed to obtain the colony count (CFU/ml). Swabs obtained from burn wounds were streaked on blood agar and MacConkey agar for bacterial isolation and identification. Later, antibacterial activity of Aquacel-tualang honey, Aquacel-Manuka honey, Aquacel-Ag and Aquacel- plain dressings against bacteria isolated from patients were tested (in-vitro) to see the effectiveness of those dressings by zone of inhibition assays.

Results: Seven organisms were isolated. Four types of Gram-negative bacteria, namely Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas spp. and Acinetobacter spp., and three Gram-positive bacteria, namely Staphylococcus aureus, coagulase-negative Staphylococcus aureus (CONS) and Streptococcus spp., were isolated. Total bacterial count decreased on day 6 and onwards. In the in-vitro antibacterial study, Aquacel-Ag and Aquacel-Manuka honey dressings gave better zone of inhibition for Gram positive bacteria compared to Aquacel-Tualang honey dressing. However, comparable results were obtained against Gram negative bacteria tested with Aquacel-Manuka honey and Aquacel-Tualang honey dressing.

Conclusions: Tualang honey has a bactericidal as well as bacteriostatic effect. It is useful as a dressing, as it is easier to apply and is less sticky compared to Manuka honey. However, for Gram positive bacteria, tualang honey is not as effective as usual care products such as silver-based dressing or medical grade honey dressing.

References

    1. Dunford CCR, Molan P, White R. The use of honey in wound management. Nursing Standard. 2000;15:63–68.
    1. Tan HT, Abdul Rahman R, Gan SH, Halim AS, Hassan SA, Sulaiman SA, Kirnpal-Kaur BS. The antibacterial properties of Malaysian Tualang honey against wound and enteric microorganisms in comparison to Manuka honey. BMC Complementary and Alternative Medicine. 2009;9(34):1–8.
    1. Cooper RA, Molan PC, Harding KG. Antibacterial activity of honey against strains of Staphylococcus aureus from infected wound. Journal of Society of Medicine. 1999;92:283–285.
    1. Cooper RA, Molan PC. The use of honey as an antiseptic in the managing pseudomonas infection. Journal of Wound Care. 1999;8(6):161–164.
    1. Molan P. A brief review of the use of honey as a clinical dressing. Australian J of Management. 1998;6:148–158.
    1. Aljady AMKMY, Jamal AM, Mohd Yassim MY. Biochemical Study on the Efficacy of Malaysian Honey on Inflicted Wounds: An Animal Model. Medical Journal of Islamic Academy of Sciences. 2000;13(3):125–132.
    1. Jeffrey AE, Echazarreta CM. Medical uses of honey. Rev Biomed. 1996;7:43–49.
    1. Lusby P, Coombes AL, Wilkinson JM. Bactericidal activity of different honeys against pathogenic bacteria. Archives of Medical Reaseach. 2005;36:464–467. doi: 10.1016/j.arcmed.2005.03.038.
    1. Pieper B. Honey-Based Dressings and Wound Care. An Option for care in the Uniter States. Journal Wound Ostomy Continence Nursing. 2009;36(1):60–66.
    1. Atiyeh BSCM, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: Review of the literature. Burns. 2007;33:139–148. doi: 10.1016/j.burns.2006.06.010.
    1. Wright JBLK, Hansen D, Burrel RE. Efficacy of topical silver against fungal burn wound pathogens. American Journal of Infection Control. 1999;27:344–350. doi: 10.1016/S0196-6553(99)70055-6.
    1. Wright JBLK, Burrel RE. Wound management in an era of increasing bacterial antibiotic resistance: A role for topical silver treatment. American Journal of Infection Control. 1998;26:572–577. doi: 10.1053/ic.1998.v26.a93527.
    1. Molan P. The Evidence Supporting the Use of Honey as a Wound Dressing. Lower Extremity Wounds. 2006;5(1):40–54. doi: 10.1177/1534734605286014.
    1. Basualdo C, Sgroy V, Finola MS, Marioli JM. Comparison of the Antibacterial Activity of Honey from Different Provenance Against Bacteria Usually Isolated from Skin Wound. Veterinary Microbiology. 2007;124:375–381. doi: 10.1016/j.vetmic.2007.04.039.
    1. Mulu A, Tessema B, Derbie F. In vitro assessment of the antimicrobial potential of honey on common human pathogen. Ethiop J Health Dev. 2004;18(2):107–112.
    1. Johnson RMR. R.M.S: Prtial Thickness Burns: Identification and Maangemenat. Advances in Skin and Wound. 2003;16(4):178–187. doi: 10.1097/00129334-200307000-00010.
    1. Shubangi Bhende. In-Vitro Assessment of Chlorhexidine Gluconate-Impregnated Polyurethane Foam Antimicrobial Dressing Using Zone of Inhibition Assay. Infection Control and Hospital Epidemiology. 2004;25(8):664–667. doi: 10.1086/502458.
    1. Molan P. Honey as an antimicrobial agent. New York: Plenum Press; 1997.
    1. Danilla SAP, Gomez ME, Chamorro M, Leniz P, Pineros JL, Lianos S, Roco H, Correa G, Pasten JA, Eulufi A, Roa R, Calderon W. Concordance between qualitative and quantitative culture in burned patients analysis of 2008 cultures. Burns. 2005;31:967–971. doi: 10.1016/j.burns.2005.08.019.
    1. Steer JA, Papini R.P.G, Wilson A.P.R, McGrouther GA, Parkhouse N. Quantitative microbiology in the management of burn patient. II. Relationship between bacterial counts obtained by burn wound biopsy culture and surface alginate swab culture, with clinical outcome following burn surgery and change of dressings. Burns. 1996;22(3):177–181. doi: 10.1016/0305-4179(95)00117-4.
    1. Church DES, Reid O, Winston B, Lindsay R. Burn Wound Infection. Clinical Microbiology Review. 2006;19(2):403–434. doi: 10.1128/CMR.19.2.403-434.2006.
    1. Gosh K, Clark R.A.F. Principle of tissue engineering. 3. Elsevier B.V; 1997.
    1. Nasser SMA, Maher A. Colonization of burn wounds in Ain Shams Burn Unit. Burns. 2003;29:229–233. doi: 10.1016/S0305-4179(02)00285-1.
    1. Seal DV, Hay RJ, Middleton KR. Skin and wound infection investigation and treatment in practice. United Kingdom: Martin Dunitz Ltd; 2000.
    1. Dastouri MR, Fakhimzadeh K, Shayeg J, Dolgari-Sharaf J, Valilou MR, Maheri-Sis N. Evaluating antibacterial activity of the Iranian honey through MIC method on some dermal and intestinal pathogenic bacteria. J of Animal and Veterinary Advances. 2008;7(4):409–412.
    1. Jones S, Bowler PG, Walker M. Antimicrobial activity of silver-containing dressing in influenced by dressing conformability with a wound surface. Wounds. 2005;17(9):263–270.

Source: PubMed

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