Impact of soluble HLA-G levels and endometrial NK cells in uterine flushing samples from primary and secondary unexplained infertile women

Roberta Rizzo, Giuseppe Lo Monte, Daria Bortolotti, Angela Graziano, Valentina Gentili, Dario Di Luca, Roberto Marci, Roberta Rizzo, Giuseppe Lo Monte, Daria Bortolotti, Angela Graziano, Valentina Gentili, Dario Di Luca, Roberto Marci

Abstract

The aim of this research was to determine the levels of human leukocyte antigen G (HLA-G) and endometrial Natural Killer ((e)NK) cell percentages in uterine flushing samples from primary and secondary infertile women. sHLA-G levels were lower in the uterine flushing samples from primary infertile women in comparison with women with secondary infertility. Lower CD56+KIR2DL4+ (e)NK cell percentages were detected in primary infertile women compared with secondary infertile women. This is the first study demonstrating that primary and secondary unexplained infertilities are characterized by different basal sHLA-G levels and CD56+KIR2DL4+ (e)NK cell percentages.

Figures

Figure 1
Figure 1
sHLA-G levels (mean ± SD) in primary and secondary infertility. sHLA-G levels were normalized for flushing volume and total protein content. * Student t test.
Figure 2
Figure 2
(a) Number of CD56brightCD16−KIR2DL4+ (e)NK cells (mean ± SD) in flushing samples from primary and secondary infertile women. * Student t test; (b) Number of CD56dimCD16−KIR2DL4+ (e)NK cells (mean ± SD) in flushing samples from primary and secondary infertile women. * Student t test.

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Source: PubMed

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