The retina rapidly incorporates ingested C20-D₃-vitamin A in a swine model

Doina M Mihai, Hongfeng Jiang, William S Blaner, Alexander Romanov, Ilyas Washington, Doina M Mihai, Hongfeng Jiang, William S Blaner, Alexander Romanov, Ilyas Washington

Abstract

Purpose: To determine how the retina uses vitamin A for vision, we studied the flux of oral vitamin A into and out of the swine retina.

Methods: We administered labeled vitamin A to swine daily for 30 days and measured the percent of the labeled vitamin A to native unlabeled vitamin A in the retinal epithelium, neuroretina, plasma, liver, lung, and kidney.

Results: We show that during normal vitamin A homeostasis, the retina rapidly assimilates newly ingested dietary vitamin A, which replaces native vitamin A. Retinal vitamin A is turned over faster than previously thought. Provitamin A carotenoids do not significantly contribute to retinal vitamin A pools when consuming diets adequate in vitamin A.

Conclusions: Fast vitamin A turnover in the retina has direct implications for emerging therapies to prevent major forms of blindness based on controlling the concentrations of retinal vitamin A.

Figures

Figure 1
Figure 1
Vitamin A absorption and retina uptake Vitamin A is denoted as lollipops. RBP: Retinol binding protein. RE: retinal epithelium. Dietary vitamin A is absorbed by the intestine and packaged into chylomicrons, which are secreted into the circulation. Plasma chylomicrons can deliver vitamin A to tissues or to the liver where vitamin A is stored and rereleased into circulation as retinol bound to its binding protein (RBP) for tissue uptake.

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