A proof-of-concept clinical study examining the NRF2 activator sulforaphane against neutrophilic airway inflammation

Charity G Duran, Allison J Burbank, Katherine H Mills, Heather R Duckworth, Maria M Aleman, Matthew J Kesic, David B Peden, Yinghao Pan, Haibo Zhou, Michelle L Hernandez, Charity G Duran, Allison J Burbank, Katherine H Mills, Heather R Duckworth, Maria M Aleman, Matthew J Kesic, David B Peden, Yinghao Pan, Haibo Zhou, Michelle L Hernandez

Abstract

Sulforaphane (SFN), a naturally occurring isothiocyanate found in cruciferous vegetables, is implicated as a possible therapy for airway inflammation via induction of the transcription factor NF-E2-related factor 2 (NRF2). In this proof-of-concept clinical study, we show that supplementation of SFN with broccoli sprout homogenate in healthy human subjects did not induce expression of antioxidant genes or protect against neutrophilic airway inflammation in an ozone-exposure model. Therefore, dietary sulforaphane supplementation is not a promising candidate for larger scale clinical trials targeting airway inflammation.

Trial registration: NCT01625130 . Registered 19 June, 2012.

Keywords: Airway inflammation; Antioxidants; NRF2; Ozone; Sulforaphane.

Figures

Fig. 1
Fig. 1
Plasma levels of SFN and its major metabolites SFN-N-acetylcysteine and SFN-glutathione following BSH supplementation (a). O3-induced changes in sputum neutrophil counts with placebo or SFN supplementation (N = 14) (b). Changes are presented as PMNs/mg sputum and %PMNs/mg sputum

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Source: PubMed

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