Satiety Evaluation of Mature Okra Flour-incorporated Biscuit (MOFB)
3 juni 2026 bijgewerkt door: Tan Chee Yee, Universiti Putra Malaysia
Evaluating the Impact of Mature Okra Flour-Incorporated Biscuits (MOFB) on Perceived Satiety Through the Satiety Labeled Intensity Magnitude (SLIM) Scale and Metabolomic Approach
This study aims to evaluate whether eating biscuits made with mature okra flour can help young adults feel fuller (enhance satiety) and change certain blood markers compared to eating standard biscuits.
Mature okra is a nutrient-dense agricultural by-product that is often discarded due to its tough texture. For this study, mature okra pods are dried and ground into a flour, which is then used to replace 10% of the wheat flour in a custom biscuit formulation.
Participants will take part in a single-blind, randomised trial where they will consume either the okra-incorporated biscuits or the standard control biscuits on separate occasions. Researchers will measure participants' perceived fullness over 165 minutes using a visual rating scale. Blood samples will also be collected at specific intervals during this time to evaluate blood glucose levels, appetite-related hormones, and metabolic responses.
Studie Overzicht
Toestand
Werving
Interventie / Behandeling
Gedetailleerde beschrijving
Okra is one of the top ten most consumed vegetable crops by Malaysians, with 1.7kg per capita consumption as reported by the Department of Statistic Malaysia in 2023.
Among the total 65.6 thousand tonnes of output available, around 7.7% of the okra is turned into waste and partially fed to the livestock.
Mature okra becomes unmarketable and turns into waste because it develops a tougher, more fibrous texture and a less desirable taste, unlike tender, immature okra, which is typically preferred for consumption.
Studies in the literature found that mature okra also possesses comparable nutrient content or is even better than immature okra in certain aspects.
Incorporation of agricultural waste, for instance, mature okra, into food products can offer significant health advantages besides reducing waste and also promotes a sustainable future, aligning with the objectives of Sustainable Development Goals (SDGs) goal No.12.
Besides, transforming mature okra and other agricultural by-products into functional or value-added foods is an urge to address environmental and unsustainable use of natural resources.
This study aims to evaluate the impact of mature okra flour-incorporated biscuits (MOFB) on perceived satiety and metabolomic responses.
Mature okra flour will be prepared by dehydrating and grinding okra pods, then incorporated into biscuits at 10% substitution of wheat flour.
This ratio of mature okra flour incorporation is pre-determined based on the previous phase study, as this ratio of incorporation results in a better nutritional profile and an acceptable sensorial score.
Subsequently, thirty healthy young adults will participate in a single-blind, randomised trial comparing MOFB and control biscuits.
Satiety will be assessed using the SLIM scale and blood glucose measurements over 165 minutes post-consumption.
Blood samples will be collected for hormone and metabolomic analyses using ELISA, RIA, and Liquid Chromatography-Mass Spectrometry (LC-MS).
The findings will help determine whether MOFB enhances satiety and alters metabolic profiles, supporting its potential as a functional food for appetite regulation.
Studietype
Ingrijpend
Inschrijving (Geschat)
30
Fase
- Niet toepasbaar
Contacten en locaties
In dit gedeelte vindt u de contactgegevens van degenen die het onderzoek uitvoeren en informatie over waar dit onderzoek wordt uitgevoerd.
Studiecontact
- Naam: Chee Yee Tan
- Telefoonnummer: +60134904250
- E-mail: cheeyee721@gmail.com
Studie Contact Back-up
- Naam: Mohd Redzwan Sabran, PhD
- Telefoonnummer: +60397692766
- E-mail: mohdredzwan@upm.edu.my
Studie Locaties
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Selangor
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Serdang, Selangor, Maleisië, 43400
- Werving
- Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM)
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Contact:
- Chee Yee Tan
- Telefoonnummer: +60134904250
- E-mail: cheeyee721@gmail.com
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Hoofdonderzoeker:
- Mohd Redzwan Sabran, PhD
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Onderonderzoeker:
- Chee Yee Tan
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Deelname Criteria
Onderzoekers zoeken naar mensen die aan een bepaalde beschrijving voldoen, de zogenaamde geschiktheidscriteria. Enkele voorbeelden van deze criteria zijn iemands algemene gezondheidstoestand of eerdere behandelingen.
Geschiktheidscriteria
Leeftijden die in aanmerking komen voor studie
- Volwassen
Accepteert gezonde vrijwilligers
Ja
Beschrijving
Inclusion Criteria:
- Young adults aged between 18 and 39 years.
- Normal Body Mass Index (BMI).
Exclusion Criteria:
- Smokers.
- Pregnant or lactating women.
- Individuals with daily medication prescriptions for chronic metabolic conditions, cardiovascular management, or hormonal regulation.
Use of daily medications/supplements that influence appetite regulation, metabolic signalling, or systemic metabolomic profiles, including:
- Hormonal contraceptives, glucocorticoids, and oral/injectable anti-obesity medications (e.g., GLP-1 agonists).
- Antihypertensives and lipid-lowering agents (statins).
- Chronic Nonsteroidal Anti-Inflammatory Drugs (NSAIDs).
- Daily fibre supplements.
Studie plan
Dit gedeelte bevat details van het studieplan, inclusief hoe de studie is opgezet en wat de studie meet.
Hoe is de studie opgezet?
Ontwerpdetails
- Primair doel: Ander
- Toewijzing: Gerandomiseerd
- Interventioneel model: Parallelle opdracht
- Masker: Enkel
Wapens en interventies
Deelnemersgroep / Arm |
Interventie / Behandeling |
|---|---|
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Experimenteel: Mature Okra Flour-incorporated Biscuit (MOFB) Group
Young adult participants assigned to this group will consume the functional biscuit formulation incorporated with mature okra flour.
Perceived satiety, metabolomic profiles, and appetite signalling will be assessed post-ingestion.
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Standard biscuit formulation manufactured where 10% of the wheat flour is substituted with mature okra flour, designed to enhance the nutritional profile while maintaining acceptable sensory scores.
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Actieve vergelijker: Control Biscuit Group
Young adult participants assigned to this group will consume the standard control biscuit formulation made with 100% wheat flour.
Perceived satiety, metabolomic profiles, and appetite signalling will be assessed post-ingestion.
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Standard biscuit formulation manufactured using 100% wheat flour, designed to match the sensory profile and appearance of the experimental biscuit without the inclusion of mature okra flour.
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Wat meet het onderzoek?
Primaire uitkomstmaten
Uitkomstmaat |
Maatregel Beschrijving |
Tijdsspanne |
|---|---|---|
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Satiety Response
Tijdsspanne: Baseline (0 minutes) and post-consumption at 15, 45, 75, 105, 135, and 165 minutes
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The satiety response after biscuit consumption will be determined by the Satiety Labeled Intensity Magnitude (SLIM) scale.
The SLIM scale consists of a 100mm vertical line with labelled phrases to indicate satiety: "greatest imaginable fullness", "extremely full", "very full", "moderately full", "slightly full", "neither hungry nor full", "slightly hungry", "moderately hungry", "very hungry", and "extremely hungry", and "greatest imaginable hunger".
The first satiety survey is filled before breakfast (time 0), and the remaining surveys at 15, 45, 75, 105, 135 and 165 min later.
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Baseline (0 minutes) and post-consumption at 15, 45, 75, 105, 135, and 165 minutes
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Change in Plasma Insulin Concentration
Tijdsspanne: Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Plasma concentration of insulin will be measured using an enzyme-linked immunosorbent assay (ELISA) kit.
This metric evaluates metabolic responses to the biscuit interventions.
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Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Change in Active Glucagon-Like Peptide-1 (GLP-1) Concentration
Tijdsspanne: Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Plasma concentration of active GLP-1 will be measured using an enzyme-linked immunosorbent assay (ELISA) kit.
This metric evaluates anorexigenic hormone responses to the biscuit interventions.
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Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Change in Plasma Peptide YY (PYY3-36) Concentration
Tijdsspanne: Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Plasma concentration of PYY3-36 will be measured using an enzyme-linked immunosorbent assay (ELISA) kit.
This metric evaluates anorexigenic hormone responses to the biscuit interventions.
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Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Change in Plasma Cholecystokinin (CCK) Concentration
Tijdsspanne: Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Plasma concentration of CCK will be determined using a radioimmunoassay (RIA) kit.
This metric evaluates anorexigenic hormone responses to the biscuit interventions.
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Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Change in Active Ghrelin Concentration
Tijdsspanne: Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Plasma concentration of active ghrelin will be measured using an enzyme-linked immunosorbent assay (ELISA) kit.
This metric evaluates orexigenic hormone responses to the biscuit interventions.
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Fasting state (0 minutes), 75 minutes, and 165 minutes post-consumption
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Changes in Metabolite Profiles
Tijdsspanne: Fasting state (0 minutes) and 165 minutes post-consumption
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For metabolomic analysis, the blood samples will be taken in the fasting state (0 min) and after the last satiety survey (165 min).
Metabolites will be quantified by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, and the serum samples will be prepared according to In Vitro Diagnostics Research (IVDr) standard operating procedures.
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Fasting state (0 minutes) and 165 minutes post-consumption
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Secundaire uitkomstmaten
Uitkomstmaat |
Maatregel Beschrijving |
Tijdsspanne |
|---|---|---|
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Blood Glucose Response
Tijdsspanne: Baseline (0 minutes) and post-consumption at 15, 45, 75, 105, 135, and 165 minutes
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The capillary blood glucose will be measured using the finger-prick method during the fasting state (time 0), and at 15, 45, 75, 105, 135 and 165 min later.
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Baseline (0 minutes) and post-consumption at 15, 45, 75, 105, 135, and 165 minutes
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Ad Libitum Energy Intake at a Subsequent Meal
Tijdsspanne: At the end of the satiety evaluation session (165 minutes post-biscuit consumption)
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Following the 165-minute satiety evaluation session, participants will be served an ad libitum meal consisting of fried rice and requested to consume as much as they want until comfortably full.
The weight of the food consumed will be calculated by subtracting the remaining food weight from the initial served weight using a digital weighing scale (accurate to 1.0 g).
Total energy intake will be calculated by multiplying the weight of the food consumed by the specific caloric content of the fried rice (expressed in kilocalories).
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At the end of the satiety evaluation session (165 minutes post-biscuit consumption)
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Medewerkers en onderzoekers
Hier vindt u mensen en organisaties die betrokken zijn bij dit onderzoek.
Sponsor
Onderzoekers
- Studie stoel: Mohd Redzwan Sabran, PhD, Universiti Putra Malaysia
Studie record data
Deze datums volgen de voortgang van het onderzoeksdossier en de samenvatting van de ingediende resultaten bij ClinicalTrials.gov. Studieverslagen en gerapporteerde resultaten worden beoordeeld door de National Library of Medicine (NLM) om er zeker van te zijn dat ze voldoen aan specifieke kwaliteitscontrolenormen voordat ze op de openbare website worden geplaatst.
Bestudeer belangrijke data
Studie start (Werkelijk)
15 mei 2026
Primaire voltooiing (Geschat)
25 juni 2026
Studie voltooiing (Geschat)
25 juni 2026
Studieregistratiedata
Eerst ingediend
28 mei 2026
Eerst ingediend dat voldeed aan de QC-criteria
3 juni 2026
Eerst geplaatst (Werkelijk)
8 juni 2026
Updates van studierecords
Laatste update geplaatst (Werkelijk)
8 juni 2026
Laatste update ingediend die voldeed aan QC-criteria
3 juni 2026
Laatst geverifieerd
1 juni 2026
Meer informatie
Termen gerelateerd aan deze studie
Andere studie-ID-nummers
- JKEUPM-2025-1085
- GP-IPS/2024/9813100 (Ander subsidie-/financieringsnummer: Universiti Putra Malaysia)
Plan Individuele Deelnemersgegevens (IPD)
Bent u van plan om gegevens van individuele deelnemers (IPD) te delen?
NEE
Beschrijving IPD-plan
Individual participant data will not be publicly shared to protect participant confidentiality in accordance with institutional ethics committee guidelines.
However, aggregated study data and final statistical outcomes will be made available upon reasonable request to the corresponding author.
Informatie over medicijnen en apparaten, studiedocumenten
Bestudeert een door de Amerikaanse FDA gereguleerd geneesmiddel
Nee
Bestudeert een door de Amerikaanse FDA gereguleerd apparaatproduct
Nee
Deze informatie is zonder wijzigingen rechtstreeks van de website clinicaltrials.gov gehaald. Als u verzoeken heeft om uw onderzoeksgegevens te wijzigen, te verwijderen of bij te werken, neem dan contact op met register@clinicaltrials.gov. Zodra er een wijziging wordt doorgevoerd op clinicaltrials.gov, wordt deze ook automatisch bijgewerkt op onze website .