Substrate Utilization and Hormonal Status in Women
Impact of Women Hormonal Status (Oral Contraception vs no Contraception) on Substrate Utilization During Exercise
Women sexual hormones (i.e. estrogen) are involved in the regulation of substrate utilization and storage. Being under oral contraception (OC) may conduct to metabolic and hormonal modifications, but results are confused regarding the impact on substrate utilization. In the present study, the aim is then to explore the effect of hormonal status on substrate utilization during sous-maximal exercise, comparing women with and without OC.
We hypothesised that women under OC have a lower Respiratory Exchange Ratio (RER) at exercise than women without contraception underlying a greater reliance on fat oxidation.
Visão geral do estudo
Status
Condições
Descrição detalhada
24 healthy young women (12 with OC and 12 without any contraception) will complete a maximal test on an ergometer cycle to obtain their maximal oxygen consumption (VO2max).Their body composition will be assessed by DXA. They will be asked to complete 3 experimental sessions.
During a first session the volunteers will remain inactive, and only a breakfast will be offered. During the second session, a breakfast will be offered and they will have to complete a sub-maximal exercise set at 60% of their VO2max 3 hours after breakfast. And the third session will consist in the realisation of exercise on fast state. Those three sessions will be realized in a randomized order. Several blood (catheter) and salivary samples will be collected during the three sessions, throughout the experimental day.
Tipo de estudo
Inscrição (Antecipado)
Contactos e Locais
Locais de estudo
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Clermont-Ferrand, França, 63003
- CHU clermont-ferrand
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Critérios de participação
Critérios de elegibilidade
Idades elegíveis para estudo
Aceita Voluntários Saudáveis
Gêneros Elegíveis para o Estudo
Método de amostragem
População do estudo
Descrição
Inclusion Criteria:
- Female
- Adults between 19 to 30 years old
- Body mass index between 20 and 25 kg.m-²
- Oral contraception (half of the sample) : mini-dosed, and mono-phasic, ethinyl estradiol ≤ 30 µg
- Without contraception (half of the sample)
- Affiliated to National Health Insurance
- Subject giving her written informed consent
- Subject considered as normal after clinical examination and medical questionnaire.
Exclusion Criteria:
- Chronic pathologies : cardiovascular diseases, cancer, chronic inflammation diseases, renal, intestinal impairments
- Refusal to be registered on the National Volunteers Data file
- Being in exclusion on the National Volunteers Data file
- Practising intensive physical exercise
- Heavy consumer of alcohol or/and tobacco
- Previous medical and/or surgery judged by the investigator as incompatible with this study
- Pregnancy
Plano de estudo
Como o estudo é projetado?
Coortes e Intervenções
Grupo / Coorte |
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12 women with oral contraception
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12 women without any contraception
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O que o estudo está medindo?
Medidas de resultados primários
Medida de resultado |
Descrição da medida |
Prazo |
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Substrate utilization will be the main outcome under study.For each volunteer, it will be assessed during exercise (between 12h and 12h45) for the 2 experimental conditions with exercise
Prazo: during exercise (between 12h and 12h45)
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Substrate utilization: The Respiratory Exchange Ratio (RER) that corresponds to carbon dioxide production divided by oxygen consumption (VCO2/VO2) will be used to determine the energetic substrate used during exercise. This RER will be obtained, in continuous, thanks to an indirect calorimetric system composed of a breath by breath gas analyzer. A RER closed to 0.7 illustrates lipid utilization while carbohydrate use will correspond to a RER that reached 1. |
during exercise (between 12h and 12h45)
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Medidas de resultados secundários
Medida de resultado |
Descrição da medida |
Prazo |
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The hormonal regulation involved in substrate utilization. It will be assessed during the 3 experimental conditions during all the experimental day: - Breakfast + exercise - Exercise alone - Breakfast alone
Prazo: before (8h, 8h45), during (12h, 12h20, 12h30, 12h45) and after exercise (13h15, 13h45; 14h45)
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The hormonal regulations involved in the substrate utilization will be assessed thanks to blood samples collected before, during and after exercise. At each blood collection, the nurses will collect a EDTA tube (5ml) used to assess free fatty acid and glycerol; and 2 heparin ones (2*5ml) to assess 17β-œstradiol, progesterone, triglycerides, cholesterol, HDL-C, LDL-C, glucose, insulin, growth hormone, IGF-I, IGFBP-1, IGFBP-3, adrenaline, noradrenaline, Atrial Natriuretic Peptide, and C-Reactive Protein. |
before (8h, 8h45), during (12h, 12h20, 12h30, 12h45) and after exercise (13h15, 13h45; 14h45)
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Colaboradores e Investigadores
Patrocinador
Investigadores
- Investigador principal: Martine DUCLOS, Pr, University Hospital, Clermont-Ferrand
Datas de registro do estudo
Datas Principais do Estudo
Início do estudo
Conclusão Primária (Real)
Conclusão do estudo (Real)
Datas de inscrição no estudo
Enviado pela primeira vez
Enviado pela primeira vez que atendeu aos critérios de CQ
Primeira postagem (Estimativa)
Atualizações de registro de estudo
Última Atualização Postada (Estimativa)
Última atualização enviada que atendeu aos critérios de controle de qualidade
Última verificação
Mais Informações
Termos relacionados a este estudo
Palavras-chave
Outros números de identificação do estudo
- CHU-0068
- AU818 (Outro identificador: CPP)
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