The Anatomical Location, Cellular Origin and Molecular Basis of Gut-derived Glucagon Secretion
研究概览
详细说明
Aim: to delineate the anatomical origin, the molecular structure and precursors, the regulation and the (patho-) physiological implications of gut-derived glucagon.
Eight totally pancreatectomised patients, eight type 2 diabetes patients and eight healthy controls (age 18-80, BMI <30) with normal kidney and liver parameters and hgb> 6.5 - will be included. The participants will each undergo one screening day and one study day including a gastroduodenoscopy. Multiple biopsies will be taken at several predefined locations in the upper gastrointestinal tract. These biopsies will be analysed with different techniques including immunohistochemical staining, quantitative polymerase chain reaction (qPCR-) analyses and more to search for glucagon. After the last biopsy has been collected, 50 g of glucose dissolved in 100 ml water is infused through the enteroscope into the most proximal part of the small intestine. Hereafter blood samples for glucagon and gut hormone analyses will be collected 15, 30 and 45 min after the glucose infusion.
研究类型
注册 (实际的)
阶段
- 不适用
联系人和位置
学习地点
-
-
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Hellerup、丹麦、2900
- Center for diabetes research
-
-
参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
描述
Inclusion Criteria:
- normal kidney function, normal liver function, normal hemoglobin levels
Exclusion Criteria:
- diabetes type 1, first degree relatives with type 2 diabetes
学习计划
研究是如何设计的？
设计细节
- 主要用途：基础科学
- 分配：非随机化
- 介入模型：并行分配
- 屏蔽：无（打开标签）
武器和干预
参与者组/臂 |
干预/治疗 |
|---|---|
|
有源比较器：Totally pancreatectomised patients
Oral glucose tolerance test and biopsies
|
Oral glucose tolerance test and biopsies
|
|
有源比较器：Type 2 diabetes patients
Oral glucose tolerance test and biopsies
|
Oral glucose tolerance test and biopsies
|
|
有源比较器：Healthy control subjects
Oral glucose tolerance test and biopsies
|
Oral glucose tolerance test and biopsies
|
研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
gut-derived glucagon
大体时间：1 year
|
The primary objective is to investigate mucosal biopsies from specific regions of the upper gastrointestinal tract
|
1 year
|
次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
Oral glucose tolerance test
大体时间：45 minutes
|
Glucose metabolism - hormones, insulin, glucagon, glp-1
|
45 minutes
|
合作者和调查者
调查人员
- 研究主任：Filip Knop, MD, PhD、University Hospital, Gentofte, Copenhagen
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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