A plant-based diet in overweight individuals in a 16-week randomized clinical trial: metabolic benefits of plant protein

Hana Kahleova, Rebecca Fleeman, Adela Hlozkova, Richard Holubkov, Neal D Barnard, Hana Kahleova, Rebecca Fleeman, Adela Hlozkova, Richard Holubkov, Neal D Barnard

Abstract

Background and objectives: A plant-based diet is an effective strategy in the treatment of obesity. In this 16-week randomized clinical trial, we tested the effect of a plant-based diet on body composition and insulin resistance. As a part of this trial, we investigated the role of plant protein on these outcomes.

Subjects and methods: Overweight participants (n = 75) were randomized to follow a plant-based (n = 38) or a control diet (n = 37). Dual X-ray Absorptiometry assessed body composition, Homeostasis Model Assessment (HOMA-IR) assessed insulin resistance, and a linear regression model was used to test the relationship between protein intake, body composition, and insulin resistance.

Results: The plant-based vegan diet proved to be superior to the control diet in improving body weight, fat mass, and insulin resistance markers. Only the vegan group showed significant reductions in body weight (treatment effect -6.5 [95% CI -8.9 to -4.1] kg; Gxt, p < 0.001), fat mass (treatment effect -4.3 [95% CI -5.4 to -3.2] kg; Gxt, p < 0.001), and HOMA-IR (treatment effect -1.0 [95% CI -1.2 to -0.8]; Gxt, p = 0.004). The decrease in fat mass was associated with an increased intake of plant protein and decreased intake of animal protein (r = -0.30, p = 0.011; and r = +0.39, p = 0.001, respectively). In particular, decreased % leucine intake was associated with a decrease in fat mass (r = +0.40; p < 0.001), in both unadjusted and adjusted models for changes in BMI and energy intake. In addition, decreased % histidine intake was associated with a decrease in insulin resistance (r = +0.38; p = 0.003), also independent of changes in BMI and energy intake.

Conclusions: These findings provide evidence that plant protein, as a part of a plant-based diet, and the resulting limitation of leucine and histidine intake are associated with improvements in body composition and reductions in both body weight and insulin resistance.

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Changes in amino acid (AA) profiles between vegans and controls at baseline and 16 weeks. (A) Control Baseline, (B) Vegan Baseline (C) Control Final, (D) Vegan Final
Fig. 2
Fig. 2
Changes in body weight, fat mass, and insulin resistance in the vegan and control group at baseline and after 16 weeks. (A) Body Mass Index (BMI), Gxt p 

Fig. 3

Regression models for changes in…

Fig. 3

Regression models for changes in (A) Animal protein and fat mass: r =…

Fig. 3
Regression models for changes in (A) Animal protein and fat mass: r = +0.39; p = 0.001; (B) Vegetable protein and fat mass: r = -0.30; p = 0.011; (C) % Leucine intake and fat mass: r = +0.40; p 
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Fig. 3
Fig. 3
Regression models for changes in (A) Animal protein and fat mass: r = +0.39; p = 0.001; (B) Vegetable protein and fat mass: r = -0.30; p = 0.011; (C) % Leucine intake and fat mass: r = +0.40; p 

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