Assessment of antioxidants status and superoxide dismutase activity in HIV-infected children

Camila Pugliese, Rose Vega Patin, Cecilia Zanin Palchetti, Cristiane Chiantelli Claudio, Aída de Fátima Thomé Barbosa Gouvêa, Regina Célia de Menezes Succi, Olga Maria Silverio Amancio, Silvia Maria Franciscato Cozzolino, Fernanda Luisa Ceragioli Oliveira, Camila Pugliese, Rose Vega Patin, Cecilia Zanin Palchetti, Cristiane Chiantelli Claudio, Aída de Fátima Thomé Barbosa Gouvêa, Regina Célia de Menezes Succi, Olga Maria Silverio Amancio, Silvia Maria Franciscato Cozzolino, Fernanda Luisa Ceragioli Oliveira

Abstract

Objective: This study aims to assess the nutritional status of selenium, copper and zinc; and also the erythrocyte superoxide dismutase activity of HIV-infected children compared to a control group.

Methods: A cross-sectional study was carried out with prepubertal HIV-infected children (n=51) and their healthy siblings (n=32). All biochemical measurements including plasma selenium, serum copper levels, serum and erythrocyte zinc levels and erythrocyte superoxide dismutase activity were evaluated according to dietary, clinical and biochemical parameters.

Results: Compared to the control group, the HIV-infected children had lower z-score values for height-for-age (p=0.0006), higher prevalence of stunting (11.8%) (p=0.047), lower selenium levels (p=0.0006) and higher copper levels (p=0.019). No difference was found concerning superoxide dismutase activity (p>0.05). The HIV-infected group presented a higher proportion (45.1%) of children with zinc intakes below the estimated average requirement (p=0.014); however, no association with zinc biochemical parameters was found.

Conclusion: HIV-infected children have an inadequate selenium and copper nutritional status, which could influence the progression to AIDS. An adequate micronutrient status could improve the clinical conditions in these patients and minimize free radical production and cellular oxidative stress.

Keywords: Antioxidants; HIV and nutrition; Oxidative stress; Trace elements.

Copyright © 2014. Published by Elsevier Editora Ltda.

References

    1. Dworkin B.M. Selenium deficiency in HIV infection and the acquired immunodeficiency syndrome (AIDS) Chem Biol Interact. 1994;91:181–186.
    1. Seifried H.E., Anderson D.E., Sorkin B.C., Costello R.B. Free radicals: the pros and cons of antioxidants. American Society for Nutritional Sciences. J Nutr. 2004;134:3143–3163.
    1. Maggini S., Wintergerst E.S., Beveridge S., Hornig D.H. Selected vitamins and trace elements support immune function by strengthening epithelial barriers and cellular and humoral immune responses. Br J Nutr. 2007;98:29–35.
    1. Campa A., Shor-Posner G., Indacochea F., et al. Mortality risk in selenium-deficient HIV-positive children. J Acquir Immune Defic Syndr Hum Retrovirol. 1999;20:508–513.
    1. Kassu A., Yabutani T., Mohammad A., et al. Alterations in serum levels of trace elements in tuberculosis and HIV infections. Eur J Clin Nutr. 2006;60:580–586.
    1. Ishigami N., Isoda K., Adachi T., et al. Deficiency of CuZn superoxide dismutase promotes inflammation and alters medial structure following vascular injury. J Atheroscler Thromb. 2011;18:1009–1017.
    1. Catania A.S., Barros C.R., Ferreira S.R.G. Vitamins and minerals with antioxidant properties and cardiometabolic risk: controversies and perspectives. Arq Bras Endocrinol Metab. 2009;53:550–559.
    1. Marshall E.A., Tanner J.M. Growth and physiological development during adolescence. Ann Rev Med. 1975;19:283–300.
    1. Centers for Disease Control Prevention Revised classification system for human immunodeficiency virus infection in children less than 13 years of age. MMWR Recomm Rep. 1994;43:1–10.
    1. WHO . World Health Organization; Geneva, Switzerland: 2007. The WHO child growth standards. Growth reference, 5–19y.
    1. Smit E., Tang A. Nutritional assessment in intravenous drug users with HIV/AIDS. J Acquir Immune Defic Syndr. 2000;25:62–69.
    1. Institute of Medicine . National Academy Press; Washington, DC: 2002. Dietary reference intakes for energy, carbohydrate, fiber, fat, protein and amino acids (macronutrients)
    1. Institute of Medicine . National Academy Press; Washington, DC: 2001. Dietary reference intakes: vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybidenum, nickel, silicon, vanadium and zinc.
    1. Hao D., Xie G., Zhang Y., Tian G. Determination of serum selenium by hydride generation flame atomic absorption spectrometry. Talanta. 1996;43:595–600.
    1. Bates C.J., Thane C.W., Prentice A., Delves H.T., Gregory J. Selenium status and associated factors in a British National Diet and Nutrition Survey: young people aged 4–18 y. Eur J Clin Nutr. 2002;56:873–881.
    1. Whitehouse R., Prasad A.S., Rabbani P.I., Cossak Z.T. Zinc in plasma, neutrophils, lymphocytes and erythrocytes as determined by flameless atomic absorption spectrophotometry. Clin Chem. 1982;28:475–480.
    1. Gibson R.S. In: Principles of nutrition assessment. 2nd ed. Gibson R.S., editor. Oxford University Press; New York: 1990. Assessment of trace-element status.
    1. Guthrie H.A., Picciano M. Human nutrition. Mosby; St Louis: 1995. Micronutrient mineral.
    1. McCord J.M., Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein) J Biol Chem. 1969;244:6049–6055.
    1. Grinspoon S.K., Grunfeld C., Kotler D.P., et al. State of the science conference: initiative to decrease cardiovascular risk and increase quality of care for patients living with HIV/AIDS: executive summary. Circulation. 2008;118:198–210.
    1. Allard J.P., Aghdassi E., Chau J., Salit I., Walmsley S. Oxidative stress and plasma antioxidant micronutrients in humans with HIV infection. Am J Clin Nutr. 1998;67:143–147.
    1. Look M.P., Rockstroh J.K., Rao G.S., et al. Serum selenium, plasma glutathione (GSH) and erythrocyte glutathione peroxidase (GSH-Px)-levels in asymptomatic versus symptomatic human immunodeficiency virus-1 (HIV-1)-infection. Eur J Clin Nutr. 1997;51:266–272.
    1. Stephensen C.B., Marquis G.S., Douglas S.D., Kruzich L.A., Wilson C.M. Glutathione, glutathione peroxidase, and selenium status in HIV-positive and HIV-negative adolescents and young adults. Am J Clin Nutr. 2007;85:173–181.
    1. Moreno T., Artacho R., Navarro M., Pérez A., Ruiz-López M.D. Serum copper concentration in HIV-infection patients and relationships with other biochemical indices. Sci Total Environ. 1998;217:21–26.
    1. Ucar S.K., Coker M., Sözmen E., Simsek D.G., Darcan S. An association among iron, copper, zinc, and selenium, and antioxidative status in dyslipidemic pediatric patients with glycogen storage disease types IA and III. J Trace Elem Med Biol. 2010;24:42–45.
    1. Ferns G.A., Lamb D.J., Taylor A. .The possible role of copper ions in atherogenesis: the Blue Janus. Atherosclerosis. 1997;133:139–152.
    1. Mazur A., Gueux E., Bureau I., Feillet-Coudray C., Rock E., Rayssiquier Y. Copper deficiency and lipoprotein oxidation. Atherosclerosis. 1998;137:443–445.

Source: PubMed

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