Evaluation of serum interleukins-6, 8 and 10 levels as diagnostic markers of neonatal infection and possibility of mortality

Hassan Boskabadi, Gholamali Maamouri, Jalil Tavakol Afshari, Shahin Mafinejad, Golkoo Hosseini, Hesam Mostafavi-Toroghi, Hamidreza Saber, Majid Ghayour-Mobarhan, Gordon Ferns, Hassan Boskabadi, Gholamali Maamouri, Jalil Tavakol Afshari, Shahin Mafinejad, Golkoo Hosseini, Hesam Mostafavi-Toroghi, Hamidreza Saber, Majid Ghayour-Mobarhan, Gordon Ferns

Abstract

Bacterial infection contributes substantially to neonatal morbidity and mortality. Early diagnosis of neonatal sepsis is difficult because clinical signs are non-specific. We have evaluated serum IL-6, 8 and 10 as potential early diagnostic markers of neonatal infection and their relationship to mortality rate and poor prognosis. Materials and Methods : A total of 84 infants, aged ≥ 72 hr were enrolled in this prospective case-control trial. The case group (n=41) included babies with clinical and laboratory findings compatible with sepsis and/or positive blood or cerebrospinal fluid cultures. The control group (n=43) included healthy infants. IL-6, 8 and 10 were measured for all infants. Receiver-operating characteristic (ROC) curves were used for the determination of thresholds. Results : Statistically significant differences were observed between control and case groups for serum median level of IL-6, 8 and 10 (P<0.001). IL-6 cut-off values of 10.85 Pg/ml for discriminating between cases and controls and 78.2 Pg/ml for predicting mortality are suggested. IL-8 at a cut-off value of 60.05 Pg/ml was valuable for differentiation of definite versus indefinite infection. Conclusion : Evaluating the IL-6, 8 and 10 simultaneously, could improve the sensitivity and specificity of early diagnosis of the neonatal sepsis. Regarding our results, interleukin 6 had the greatest value for predicting infection and possible mortality, whereas IL-8 was valuable for diagnosing definitive infection.

Keywords: Infection; Interleukin-10; Interleukin-6; Interleukin-8; Newborn; Sepsis.

Figures

Figure 1
Figure 1
ROC curves indicating sensitivity and specificity cut-offs of IL-6, 8 and 10 for predicting case and control subjects. IL-6 at a cut off value of 10.85 Pg/ml and IL-8 at a cut off value of 64.05 Pg/ml

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Source: PubMed

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