Early administration of Bifidobacterium breve to preterm infants: randomised controlled trial

H Kitajima, Y Sumida, R Tanaka, N Yuki, H Takayama, M Fujimura, H Kitajima, Y Sumida, R Tanaka, N Yuki, H Takayama, M Fujimura

Abstract

Aim: To investigate the colonisation with Bifidobacterium breve of the bowels of very low birthweight (VLBW) infants.

Methods: The adverse effects of B breve were examined in 66 VLBW infants (preliminary study). A prospective randomised clinical study of 91 VLBW infants was also completed and these infants were followed up for three years. Precise viable bacterial counts of serial stool specimens were examined for the first eight weeks after birth in 10 infants. The colonisation rates of administered bacteria were examined using immunohistochemical staining of stool specimens with a B breve specific monoclonal antibody.

Results: In the preliminary study there were no side effects attributable to the bacteria. Immunohistochemical staining of stool specimens showed that the colonisation rates of the administered bacteria were 73% at 2 weeks of age, but only 12% in the control group. Early administration of B breve significantly decreased aspirated air volume from the stomach and improved weight gain.

Conclusions: B breve can colonise the immature bowel very effectively and is associated with fewer abnormal abdominal signs and better weight gain in VLBW infants, probably as a result of stabilisation of their intestinal flora and accelerated feeding schedules.

Figures

Figure 1
Figure 1
Corn starch aggregates in stool specimen of extremely low birthweight infant (24.4 weeks, 616 g, female): (A) corn starch aggregates in stool specimen; (B) aggregates broken in distilled water (× 400 magnification).
Figure 1
Figure 1
Corn starch aggregates in stool specimen of extremely low birthweight infant (24.4 weeks, 616 g, female): (A) corn starch aggregates in stool specimen; (B) aggregates broken in distilled water (× 400 magnification).
Figure 2
Figure 2
Bacterial counts from stool specimens from each infant.
Figure 3
Figure 3
Daily changes in aspirated air volume from the stomach. Bars represent mean (SEM): ***P

Figure 4

Daily changes in feeding volume:…

Figure 4

Daily changes in feeding volume: bars represent mean (SEM). *** P

Figure 4
Daily changes in feeding volume: bars represent mean (SEM). *** P 

Figure 5

Weekly changes in body weight:…

Figure 5

Weekly changes in body weight: bars represent mean (SEM). *** P

Figure 5
Weekly changes in body weight: bars represent mean (SEM). *** P 
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References
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Figure 4
Figure 4
Daily changes in feeding volume: bars represent mean (SEM). *** P 

Figure 5

Weekly changes in body weight:…

Figure 5

Weekly changes in body weight: bars represent mean (SEM). *** P

Figure 5
Weekly changes in body weight: bars represent mean (SEM). *** P 
Similar articles
Cited by
References
    1. Pediatrics. 1983 Sep;72(3):317-21 - PubMed
    1. J Hyg (Lond). 1982 Aug;89(1):59-67 - PubMed
    1. Eur J Pediatr. 1985 Jul;144(2):186-90 - PubMed
    1. Pediatr Infect Dis J. 1987 Apr;6(4):344-51 - PubMed
    1. Keio J Med. 1987 Jul;36(3):298-314 - PubMed
Show all 22 references
Publication types
MeSH terms
Related information
Full text links [x]
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM
Figure 5
Figure 5
Weekly changes in body weight: bars represent mean (SEM). *** P 

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