The role of DNA repair genes in recombination between repeated sequences in yeast

B Liefshitz, A Parket, R Maya, M Kupiec, B Liefshitz, A Parket, R Maya, M Kupiec

Abstract

The presence of repeated sequences in the genome represents a potential source of karyotypic instability. Genetic control of recombination is thus important to preserve the integrity of the genome. To investigate the genetic control of recombination between repeated sequences, we have created a series of isogenic strains in which we could assess the role of genes involved in DNA repair in two types of recombination: direct repeat recombination and ectopic gene conversion. Naturally occurring (Ty elements) and artificially constructed repeats could be compared in the same cell population. We have found that direct repeat recombination and gene conversion have different genetic requirements. The role of the RAD51, RAD52, RAD54, RAD55, and RAD57 genes, which are involved in recombinational repair, was investigated. Based on the phenotypes of single and double mutants, these genes can be divided into three functional subgroups: one composed of RAD52, a second one composed of RAD51 and RAD54, and a third one that includes the RAD55 and RAD57 genes. Among seven genes involved in excision repair tested, only RAD1 and RAD10 played a role in the types of recombination studied. We did not detect a differential effect of any rad mutation on Ty elements as compared to artificially constructed repeats.

References

    1. Genetics. 1989 Dec;123(4):683-94
    1. Nature. 1981 Jul 23;292(5821):306-11
    1. Nature. 1992 Feb 6;355(6360):555-8
    1. Genetics. 1988 Oct;120(2):367-77
    1. Genetics. 1995 Jan;139(1):109-23
    1. Genes Dev. 1994 Sep 1;8(17):2035-45
    1. Mol Cell Biol. 1986 May;6(5):1497-507
    1. Genetics. 1995 May;140(1):67-77
    1. Genetics. 1991 Jan;127(1):53-60
    1. Mol Cell Biol. 1991 Jun;11(6):3278-87
    1. Nature. 1993 Apr 29;362(6423):860-2
    1. Cell. 1992 May 1;69(3):457-70
    1. Science. 1993 Mar 26;259(5103):1896-9
    1. Genetics. 1989 May;122(1):19-27
    1. Mol Cell Biol. 1992 Mar;12(3):1292-303
    1. Genetics. 1990 Mar;124(3):547-59
    1. Annu Rev Genet. 1993;27:33-70
    1. Mol Cell Biol. 1992 Jul;12(7):3235-46
    1. Genetics. 1987 Feb;115(2):233-46
    1. Mol Cell Biol. 1992 Oct;12(10):4441-8
    1. Genetics. 1986 Jul;113(3):551-67
    1. Genetics. 1986 Nov;114(3):731-52
    1. Science. 1994 Sep 30;265(5181):2082-5
    1. Mol Cell Biol. 1994 Sep;14(9):6135-42
    1. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12711-5
    1. Mol Cell Biol. 1992 Apr;12(4):1613-20
    1. Genetics. 1987 Aug;116(4):541-5
    1. Genetics. 1989 Oct;123(2):261-8
    1. J Bacteriol. 1981 Nov;148(2):618-23
    1. Nature. 1981 Jan 15;289(5794):144-8
    1. Semin Cancer Biol. 1993 Apr;4(2):73-83
    1. Mol Cell Biol. 1988 Sep;8(9):3619-26
    1. Genetics. 1980 Dec;96(4):819-39
    1. Genes Dev. 1994 Jun 1;8(11):1344-55
    1. Methods Enzymol. 1983;101:202-11
    1. Nature. 1976 Dec 23-30;264(5588):719-22
    1. Mol Cell Biol. 1990 Jan;10(1):103-12
    1. Genetics. 1971 Sep;69(1):17-27
    1. Nature. 1981 Mar 19;290(5803):191-2
    1. Proc Natl Acad Sci U S A. 1980 Jan;77(1):503-7
    1. J Bacteriol. 1989 Apr;171(4):1862-9
    1. Mol Cell Biol. 1994 Jun;14(6):3569-76
    1. Genetics. 1980 Jan;94(1):31-50
    1. Genetics. 1989 Dec;123(4):725-38
    1. Genetics. 1994 Nov;138(3):587-95
    1. Genetics. 1987 Aug;116(4):547-53
    1. Mol Cell Biol. 1991 Mar;11(3):1222-31
    1. Microbiol Rev. 1988 Mar;52(1):70-102
    1. Mol Cell Biol. 1993 Dec;13(12):7757-65
    1. Genetics. 1988 Aug;119(4):779-90
    1. Genetics. 1992 Oct;132(2):387-402
    1. Mol Gen Genet. 1984;197(2):345-6
    1. Mol Cell Biol. 1990 Jun;10(6):2485-91
    1. Gene. 1991 Jul 31;104(1):103-6
    1. Science. 1994 Aug 26;265(5176):1241-3
    1. J Bacteriol. 1981 May;146(2):692-704
    1. Genes Dev. 1993 Sep;7(9):1755-65
    1. Genetics. 1994 Jun;137(2):393-405
    1. J Biol Chem. 1993 Dec 15;268(35):26391-9
    1. Gene. 1991 Aug 30;105(1):139-40
    1. Science. 1992 Oct 16;258(5081):480-4
    1. J Bacteriol. 1986 Jun;166(3):914-23
    1. Mol Cell Biol. 1995 Jan;15(1):19-25
    1. Gene. 1994 May 3;142(1):103-6
    1. Mutat Res. 1980 Dec;73(2):251-65
    1. Genetics. 1995 Jan;139(1):45-56
    1. Mol Cell Biol. 1988 Jul;8(7):2942-54
    1. Curr Genet. 1989 Dec;16(5-6):339-46
    1. Mol Gen Genet. 1976 Jan 16;143(2):119-29

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