Fasting plasma adropin concentrations correlate with fat consumption in human females

Abstract

Objective: This study investigated whether plasma adropin concentrations are influenced by sleep restriction and correlate with dietary preferences.

Methods: Plasma adropin concentrations were measured by ELISA using samples from a study that investigated feeding behavior in sleep deprived lean (body mass index 22-26 kg/m(2) ) men and women aged 30-45 y. Sleep (habitual or restricted to 4h/night) and diet were controlled during a 4-day inpatient period. On day 5, food was self-selected (FS). Adropin was measured on day 4 in samples collected throughout the day, and then after an overnight fast at 0730 on days 5 (Pre-FS) and 6 (Post-FS).

Results: Plasma adropin concentrations were not affected by sleep restriction. However, circulating adropin concentrations correlated with food selection preferences in women, irrespective of sleep status. Pre-FS adropin correlated positively with fat intake (total fat, r = 0.867, P < 0.05; saturated fat, r = 0.959, P < 0.01) and negatively with carbohydrate intake (r = -0.894, P < 0.05) as a percent total energy. Post-FS adropin correlated with total (r = 0.797, P < 0.05) and saturated fat intake (r = 0.945, P < 0.01), and negative with total carbohydrate intake (r = -0.929, P < 0.01). Pre-FS adropin also correlated with fat intake in kcal adjusted for body size (total fat, r = 0.852, P < 0.05; saturated fat, r = 0.927, P < 0.01).

Conclusions: Plasma adropin concentrations correlate with fat consumption in women.

Conflict of interest statement

Conflicts of interest

Competing interests: the authors have no competing interests.

Copyright © 2013 The Obesity Society.

Figures

Figure 1

Schematic of the experimental protocol (A) and food intake data during habitual (H) or sleep restriction (SR) (B). The volunteers participated in a 4 day inpatient study where diet, sleep and food intake were controlled. Serial sampling of plasma occurred on day 4. Fasting samples were collected on morning of day 5 prior to individuals given the opportunity of food selection (Pre-FS) and on day 6 (Post-FS). The impact of sleep was assessed using a randomized crossover design; in the following panels short sleep data is shown with open symbols while habitual sleep data is shown with grey-shaded symbols. Also shown are the intakes of energy in the form of protein, carbohydrates or fat expressed as kcal (B) during habitual and sleep deprivation.

Figure 2

Plasma adropin concentrations on day 4 of the inpatient study. Plasma adropin concentrations were measured in samples collected serially over a twenty-four hour period. These samples were from females subjected to restricted sleep or allowed to adhere to normal (habitual) sleep patterns. The black triangles indicate meal times (breakfast at 0800, lunch at 1200, afternoon snack at 1600, and dinner and 1800 h).

Figure 3

Scatterplots showing correlations between fasting plasma adropin concentrations on day 5 (Pre-FS) and food intake. The values on the x-axis are fasting plasma adropin concentrations measured in samples collected at 0730 on day 5. The values on the Y-axis indicate intakes of fat (A,E), carbohydrate (CHO) (B,F) and protein (C, G) expressed as calories (A–C) or percent of total energy (E–G). Food preference data was collected on day 5 in habitual or sleep restricted conditions.

Figure 4

Scatterplots showing correlations between fasting plasma adropin concentrations on day 6 (Post-FS) with day 5 intakes of fat (A,E), carbohydrate (CHO) (B,F) and protein (C, G) as calories (A–C) or percent of total energy (E–G). Food intake data was collected on day 5 in habitual or sleep restricted conditions.

Figure 5

Scatterplots showing the correlations in women between fasting plasma adropin concentrations on day 5 (A, C) and day 6 (B, D) with measures of fat intake on day 5 during habitual (open symbols, solid trendlines ) or after sleep restriction (black symbols, broken trendlines). The data shown are for total fat intake (circles) or saturated fat (triangles) in kcal (A,B) or as a percent of total energy (C, D).