Blueberry polyphenol-enriched soybean flour reduces hyperglycemia, body weight gain and serum cholesterol in mice
Diana E Roopchand, Peter Kuhn, Leonel E Rojo, Mary Ann Lila, Ilya Raskin, Diana E Roopchand, Peter Kuhn, Leonel E Rojo, Mary Ann Lila, Ilya Raskin
Abstract
Defatted soybean flour (DSF) can sorb and concentrate blueberry anthocyanins and other polyphenols, but not sugars. In this study blueberry polyphenol-enriched DSF (BB-DSF) or DSF were incorporated into very high fat diet (VHFD) formulations and provided ad libitum to obese and hyperglycemic C57BL/6 mice for 13 weeks to investigate anti-diabetic effects. Compared to the VHFD containing DSF, the diet supplemented with BB-DSF reduced weight gain by 5.6%, improved glucose tolerance, and lowered fasting blood glucose levels in mice within 7 weeks of intervention. Serum cholesterol of mice consuming the BB-DSF-supplemented diet was 13.2% lower than mice on the diet containing DSF. Compounds were eluted from DSF and BB-DSF for in vitro assays of glucose production and uptake. Compared to untreated control, doses of BB-DSF eluate containing 0.05-10μg/μL of blueberry anthocyanins significantly reduced glucose production by 24-74% in H4IIE rat hepatocytes, but did not increase glucose uptake in L6 myotubes. The results indicate that delivery of blueberry polyphenols stabilized in a high-protein food matrix may be useful for the dietary management of pre-diabetes and/or diabetes.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Figures
![Figure 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3833590/bin/nihms-431287-f0001.jpg)
![Figure 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3833590/bin/nihms-431287-f0002.jpg)
Figure 3
BB-DSF-e is hypoglycemic in C57BL/6…
Figure 3
BB-DSF-e is hypoglycemic in C57BL/6 mice. Blood glucose levels of mice before and…
Figure 4
BB-DSF-e and DSF-e repress glucose…
Figure 4
BB-DSF-e and DSF-e repress glucose production in H4IIE cells. ( A ) Release…
![Figure 3](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3833590/bin/nihms-431287-f0003.jpg)
![Figure 4](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3833590/bin/nihms-431287-f0004.jpg)
Source: PubMed