The fungal microbiota of de-novo paediatric inflammatory bowel disease

I Mukhopadhya, R Hansen, C Meharg, J M Thomson, R K Russell, S H Berry, E M El-Omar, G L Hold, I Mukhopadhya, R Hansen, C Meharg, J M Thomson, R K Russell, S H Berry, E M El-Omar, G L Hold

Abstract

Inflammatory bowel disease (IBD) is characterised by an inappropriate chronic immune response against resident gut microbes. This may be on account of distinct changes in the gut microbiota termed as dysbiosis. The role of fungi in this altered luminal environment has been scarcely reported. We studied the fungal microbiome in de-novo paediatric IBD patients utilising next generation sequencing and compared with adult disease and normal controls. We report a distinct difference in fungal species with Ascomycota predominating in control subjects compared to Basidiomycota dominance in children with IBD, which could be as a result of altered tolerance in these patients.

Keywords: Fungal microbiota; Gut microbiota; Paediatric inflammatory bowel disease.

Copyright © 2015 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Figures

Fig. 1
Fig. 1
Individual patient phylum-level diversity assessment as stacked bars.
Fig. 2
Fig. 2
Individual patient genus-level diversity assessment as stacked bars.

References

    1. Stone M.A., Mayberry J.F., Baker R. Prevalence and management of inflammatory bowel disease: a cross-sectional study from central England. Eur J Gastroenterol Hepatol. 2003;15:1275–1280.
    1. Armitage E., Drummond H.E., Wilson D.C., Ghosh S. Increasing incidence of both juvenile-onset Crohn's disease and ulcerative colitis in Scotland. Eur J Gastroenterol Hepatol. 2001;13:1439–1447.
    1. Hansen R., Thomson J.M., El-Omar E.M., Hold G.L. The role of infection in the aetiology of inflammatory bowel disease. J Gastroenterol. 2010;45:266–276.
    1. Jostins L., Ripke S., Weersma R.K., Duerr R.H., McGovern D.P., Hui K.Y. Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease. Nature. 2012;491:119–124.
    1. Henderson P., Satsangi J. Genes in inflammatory bowel disease: lessons from complex diseases. Clin Med. 2011;11:8–10.
    1. Qin J., Li R., Raes J., Arumugam M., Burgdorf K.S., Manichanh C. A human gut microbial gene catalogue established by metagenomic sequencing. Nature. 2010;464:59–65.
    1. Eckburg P.B., Bik E.M., Bernstein C.N., Purdom E., Dethlefsen L., Sargent M. Diversity of the human intestinal microbial flora. Science. 2005;308:1635–1638.
    1. Ott S.J., Kuhbacher T., Musfeldt M., Rosenstiel P., Hellmig S., Rehman A. Fungi and inflammatory bowel diseases: alterations of composition and diversity. Scand J Gastroenterol. 2008;43:831–841.
    1. Zoetendal E.G., von Wright A., Vilpponen-Salmela T., Ben-Amor K., Akkermans A.D., de Vos W.M. Mucosa-associated bacteria in the human gastrointestinal tract are uniformly distributed along the colon and differ from the community recovered from feces. Appl Environ Microbiol. 2002;68:3401–3407.
    1. Hayashi H., Sakamoto M., Benno Y. Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture-based methods. Microbiol Immunol. 2002;46:535–548.
    1. Bernhardt H., Knoke M. Mycological aspects of gastrointestinal microflora. Scand J Gastroenterol Suppl. 1997;222:102–106.
    1. Simon G.L., Gorbach S.L. Intestinal flora in health and disease. Gastroenterology. 1984;86:174–193.
    1. Kaul A., Hutfless S., Liu L., Bayless T.M., Marohn M.R., Li X. Serum anti-glycan antibody biomarkers for inflammatory bowel disease diagnosis and progression: a systematic review and meta-analysis. Inflamm Bowel Dis. 2012;18:1872–1884.
    1. Russell R.K., Ip B., Aldhous M.C., MacDougall M., Drummond H.E., Arnott I.D. Anti-Saccharomyces cerevisiae antibodies status is associated with oral involvement and disease severity in Crohn disease. J Pediatr Gastroenterol Nutr. 2009;48:161–167.
    1. Landers C.J., Cohavy O., Misra R., Yang H., Lin Y.C., Braun J. Selected loss of tolerance evidenced by Crohn's disease-associated immune responses to auto- and microbial antigens. Gastroenterology. 2002;123:689–699.
    1. Murdoch T.B., Xu W., Stempak J.M., Landers C., Targan S.R., Rotter J.I. Pattern recognition receptor and autophagy gene variants are associated with development of antimicrobial antibodies in Crohn's disease. Inflamm Bowel Dis. 2012;18:1743–1748.
    1. Hansen R., Berry S.H., Mukhopadhya I., Thomson J.M., Saunders K.A., Nicholl C.E. The microaerophilic microbiota of de-novo paediatric inflammatory bowel disease: the BISCUIT study. PLoS One. 2013;8:e58825.
    1. Thomson J.M., Hansen R., Berry S.H., Hope M.E., Murray G.I., Mukhopadhya I. Enterohepatic helicobacter in ulcerative colitis: potential pathogenic entities? PLoS One. 2011;6:e17184.
    1. Brad T., van Breukelen B.M., Braster M., van Straalen N.M., Roling W.F. Spatial heterogeneity in sediment-associated bacterial and eukaryotic communities in a landfill leachate-contaminated aquifer. FEMS Microbiol Ecol. 2008;65:534–543.
    1. Hansen R., Russell R.K., Reiff C., Louis P., McIntosh F., Berry S.H. Microbiota of de-novo pediatric IBD: increased faecalibacterium prausnitzii and reduced bacterial diversity in Crohn's but not in ulcerative colitis. Am J Gastroenterol. 2012;107:1913–1922.
    1. Mavromanolakis E., Maraki S., Cranidis A., Tselentis Y., Kontoyiannis D.P., Samonis G. The impact of norfloxacin, ciprofloxacin and ofloxacin on human gut colonization by Candida albicans. Scand J Infect Dis. 2001;33:477–478.
    1. Hoffmann C., Dollive S., Grunberg S., Chen J., Li H., Wu G.D. Archaea and fungi of the human gut microbiome: correlations with diet and bacterial residents. PLoS One. 2013;8:e66019.
    1. Standaert-Vitse A., Jouault T., Vandewalle P., Mille C., Seddik M., Sendid B. Candida albicans is an immunogen for anti-Saccharomyces cerevisiae antibody markers of Crohn's disease. Gastroenterology. 2006;130:1764–1775.

Source: PubMed

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