The Effect of Meat and Food Drived Polyphenols on Oxidize LDL Level

Nutritional Study:The Effect of Meat and Food Drived Polyphenols on Oxidize LDL Level

The postprandial increase in MDA level following meat meal could cause modification to LDL particles in healthy volunteers. Co-consumption of food drived polyphenols with the meat will result in a significant decrease in LDL modification.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Other: frozen meat cutlets meal; Other: meat meals

• Study Type: Interventional
• Enrollment (Actual): 14
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Israel
  • Jerusalem, Israel, 91120
    • The Hebrew University of Jerusalem

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 60 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Healthy adults, normal BMI

Exclusion Criteria:

• Metabolic disorders
• Smokers
• Drinkers
• Taking nutritional supplement

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Other: frozen meat; 250 g of frozen meat meal for 4 days	Other: frozen meat cutlets meal; 250 g of meat cutlets; Other: meat meals; 250 g of meat meal for 4 days
Other: frozen meat and wine; 250 g of frozen meat and red wine for 4 days	Other: frozen meat cutlets meal; 250 g of meat cutlets; Other: meat meals; 250 g of meat meal for 4 days
Other: fresh meat; 250 g of fresh meat meal	Other: frozen meat cutlets meal; 250 g of meat cutlets; Other: meat meals; 250 g of meat meal for 4 days
Other: fresh meat and wine; 250 g of fresh meat meal	Other: frozen meat cutlets meal; 250 g of meat cutlets; Other: meat meals; 250 g of meat meal for 4 days

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in plasma MDA levels following meat meal	After an overnight fast baseline (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The subjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 and 6 h after the meal was eaten. Serum was separated from whole blood by centrifugation (910 g, 15 min), and MDA was evaluated by HPLC in plasma samples	Fasting before meal and 3, and 6 h postprandial, and fasting levels
Changes in MDA concentration on LDL fraction following meat meals	Subject consumed the test meals for 4 days. Day 1, (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The sbjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 h after the meal was eaten. Blood was collected at day 4, 3 h after the meal was eaten, at the morning of day 5.Plasma LDL was isolated using a single discontinuous density gradient ultracentrifugation. The protein concentration was determined based on the method of Bradford. MDA concentration was evaluated by HPLC.	Fasting, before meal was eaten, 3 h following meal, at day 4, 3 h following meal consumption, and day 5 following over nigth fast

Collaborators and Investigators

• Sponsor: Hadassah Medical Organization
• Investigators: Study Director: Shlomit Gorelik, PhD, Hebrew University of Jerusalem

Study record dates

Study Major Dates

• Study Start: October 1, 2010
• Primary Completion (Actual): February 1, 2011
• Study Completion (Actual): May 1, 2011

Study Registration Dates

• First Submitted: July 3, 2011
• First Submitted That Met QC Criteria: July 6, 2011
• First Posted (Estimate): July 8, 2011

Study Record Updates

• Last Update Posted (Estimate): July 8, 2011
• Last Update Submitted That Met QC Criteria: July 6, 2011
• Last Verified: October 1, 2009

More Information

Terms related to this study

• Keywords: MDA; LDL; meat
• Other Study ID Numbers: MWPP09-HMO-CTIL