- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT01541592
Does Class of Dietary Fat Affect Insulin Resistance?
Insulin Resistance and Dietary Fat
It has been shown that intravenous fatty acids given to normal volunteers in the form of heparin and lipid emulsions will cause insulin resistance in a matter of a few hours. It is not known if this same phenomenon can be demonstrated with oral fat. The investigators are specifically interested in whether or not there are differences in the induction of insulin resistance between the 3 main classes of fatty acids (saturated, monounsaturated and polyunsaturated).
The investigators also plan to evaluate endothelial dysfunction and blood pressure; both of which frequently accompany insulin resistance.
Study Overview
Detailed Description
Type 2 Diabetes and cardiovascular disease are closely linked. A common abnormality in both conditions is insulin resistance. The primary cause of insulin resistance in not known. A significant question is what dietary components contribute to the development of insulin resistance. Based on epidemiologic data, it seems that the type of dietary fat is a significant contributor to the development of insulin resistance. However, some researchers argue that the main determinant is total amount of fat, not the composition.
This study is intended to determine if the main types of fat (saturated, monounsaturated and polyunsaturated) ingested over a short time can cause insulin resistance in lean, healthy people. It has been demonstrated that 6 hours of intravenous fat infused into lean, healthy subjects can result in insulin resistance, as well as blood pressure elevation and endothelial dysfunction. Whether this is so with oral fat is not known. The question is important because there is debate about whether the type of fat is as important as the quantity of fat in a person's daily diet. Settling this debate will enhance the ability of health care personnel to determine the optimal dietary recommendations.
This study will make use of a high fat diet consisting of vegetable oils high in the 3 main fatty acids in plasma (palmitate, oleate and linoleate). Because large fat loads can cause intestinal discomfort, a feeding tube will be used to bypass the stomach. The 3 oils will be assigned randomly following a base line study with saline 2 weeks prior to the intervention. Our primary endpoint is insulin resistance, which will be measured by a euglycemic, hyperinsulinemic clamp. Secondary measures will include changes in blood pressure and in vascular reactivity as measured by ultrasound after brief occlusion of a forearm vessel. The changes will be compared to baseline and to the other 2 groups.
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Locations
-
-
Minnesota
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Rochester, Minnesota, United States, 55905
- Mayo Clinic in Rochester
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- Lean,healthy adults 18 - 40
- No chronic medications (birth control pills allowed)
- Weight stable
- Normal fasting labs performed at screening
- Body mass index ≤ 25 kg/M^2 .
Exclusion Criteria:
- Insulin dependent diabetes
- Thyroid disease
- History of diabetes or heart disease
- History of type 2 diabetes in parent or sibling
Study Plan
How is the study designed?
Design Details
- Primary Purpose: BASIC_SCIENCE
- Allocation: RANDOMIZED
- Interventional Model: PARALLEL
- Masking: NONE
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
ACTIVE_COMPARATOR: Saturated fat
Palm oil (rich in the saturated fat palmitate)
|
On the study day, each participant will receive 100 grams of a randomly assigned fat via a nasoduodenal tube.
The oil will be infused at a rate of ~1 tablespoon per hour so that the total infusion time will be 6 hours.
|
ACTIVE_COMPARATOR: Monounsaturated fat
Olive oil (rich in the monounsaturated fat oleate)
|
On the study day, each participant will receive 100 grams of a randomly assigned fat via a nasoduodenal tube.
The oil will be infused at a rate of ~1 tablespoon per hour so that the total infusion time will be 6 hours.
|
ACTIVE_COMPARATOR: Polyunsaturated fat
Safflower oil (rich in the polyunsaturated fat linoleate)
|
On the study day, each participant will receive 100 grams of a randomly assigned fat via a nasoduodenal tube.
The oil will be infused at a rate of ~1 tablespoon per hour so that the total infusion time will be 6 hours.
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in Insulin Sensitivity from Baseline at Two Weeks
Time Frame: Average of 2 weeks.
|
Insulin sensitivity will be measured twice (once at baseline following a nasoduodenal infusion of saline for 6 hours; two weeks later following a nasoduodenal infusion of a vegetable oil high in one of 3 fatty acids for 6 hours) using a euglycemic, hyperinsulinemic clamp as described by R. DeFronzo.
The actual measurement is expressed as a glucose infusion rate.
|
Average of 2 weeks.
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in Endothelial Function from Baseline at Two Weeks
Time Frame: Average of two weeks
|
Endothelial function will be measured by measuring the dilation of the brachial artery in the elbow following occlusion by a blood pressure cuff, which is then released.
The actual measurement involves use of ultrasound by an investigator experienced with this technique.
|
Average of two weeks
|
Change in Systolic Blood Pressure at Two Weeks
Time Frame: Average of two weeks
|
Blood pressure will be measured using a standard automated blood pressure cuff.
The measurements will be taken several times during each study day.
|
Average of two weeks
|
Collaborators and Investigators
Sponsor
Investigators
- Principal Investigator: Robert Nelson, MD, Mayo Clinic
Study record dates
Study Major Dates
Study Start
Primary Completion (ACTUAL)
Study Completion (ACTUAL)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (ESTIMATE)
Study Record Updates
Last Update Posted (ESTIMATE)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- 11-000179
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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