Fructose-induced Intestinal de Novo Lipogenesis (FIDNL)

Measure of Intestinal Lipogenesis in Healthy Volunteers and Obese Subjects Before and After Gastroplasty: Modulation by Glucose and Fructose

8 healthy volunteers, 8 pre-bypass obese subjects, 8 subjects between 12-18 months post-bypass, and 8 subjects matched to the post-bypass patients will be studied in two sub-studies Sub-study 1) after ingestion of two or three different of the following test-meals: A)cream and whey protein B)cream, whey protein and fructose + 13C6 fructose C)test meal with cream, whey protein, fructose and glucose + 13C6 fructose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood Sub-study 2) after ingestion of two or three different of the following test-meals: A)cream labelled with 13C6 palmitic acid and whey protein B))cream labelled with 13C6 palmitic acid, whey protein, fructose and glucose

Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood in sub-study 1

Their exogenous lipid absorption kinetics will be assessed by measuring 13C palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in sub-study 2

Study Overview

• Status: Completed
• Conditions: Obesity
• Intervention / Treatment: Other: fat+protein meal; Other: Fat + protein + 13C fructose meal; Other: Fat+protein+13C fructose+glucose meal; Other: 13C Fat + whey protein; Other: 13C Fat + whey protein + glucose+ glucose meal

• Study Type: Observational
• Enrollment (Actual): 24

Contacts and Locations

Study Locations

• Switzerland
  • VD
    • Lausanne, VD, Switzerland
      • Centre Hospitalier Universitaire Vaudois

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 25 years to 50 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

8 non-obese healthy male and female subjects with BMI <25 8 obese male and female subjects with BMI> 35 8 post-gastric bypass male and female subjects 8 healthy subjects matched to post-gastric bypass subjects for age, gender and weight

Description

Inclusion Criteria:

• age 18-50 years
• sex male or female
• less than 2 kg body weight change over the past 3 months subjects

Exclusion Criteria:

• antidiabetic and hypolipemic drugs
• alcohol consumption >20g/day
• severe eating disorders
• severe psychological problems
• vegetarian diet or other specific diet
• consumption of illicit substances
• pregnancy
• weight gain or weight loss > 1 kg in the last 3 months

Study Plan

How is the study designed?

Number of groups / cohorts

3

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
non-obese healthy subjects; subjects with BMI<25 and no known disease	Other: fat+protein meal; cream+ whey protein; Other: Fat + protein + 13C fructose meal; cream+ whey protein +13C fructose; Other: Fat+protein+13C fructose+glucose meal; Cream + whey protein + glucose:13C fructose mixture; Other: 13C Fat + whey protein; cream labelled with 13C palmitate + whey protein; Other: 13C Fat + whey protein + glucose+ glucose meal; cream labelled with 13C palmitate + whey protein + glucose:fructose mixture
post-gastric bypass; post-obese subjects 12-48 months after Roux-en-Y gastric bypass	Other: fat+protein meal; cream+ whey protein; Other: Fat + protein + 13C fructose meal; cream+ whey protein +13C fructose; Other: 13C Fat + whey protein + glucose+ glucose meal; cream labelled with 13C palmitate + whey protein + glucose:fructose mixture
matched control subjects; Nonobese, healthy subjects of similar age, weight, and gender ratio, than post-bypass subjects	Other: fat+protein meal; cream+ whey protein; Other: Fat + protein + 13C fructose meal; cream+ whey protein +13C fructose; Other: 13C Fat + whey protein + glucose+ glucose meal; cream labelled with 13C palmitate + whey protein + glucose:fructose mixture

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Intestinal de novo lipogenesis (sub-study 1)	Measurement of total TG and 13C palmitate in chylomicrons: The sf >20 fraction of post-prandial blood samples will be prepared, and non-chylomicrons, apoB100-containing lipoprotein will be removes by immuno-affinity chromatography using a specific anti-apoB100 antibody	0-8-hour post-prandial
Postprandial exogenous, labelled palmitate concentration (sub-study 2)	Measurement of total TG and 13C palmitate in chylomicrons of sf >20 fraction of post-prandial blood samples will be prepared,	0-8-hour post-prandial

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
post-prandial triglyceride profile (sub-studies 1 and 2	Triglyceride will be measured in the chylomicron, VLDL, LDL and HDL fractions of post-prandial blood samples	0-8 hour post-prandial

Collaborators and Investigators

• Sponsor: University of Lausanne

Publications and helpful links

General Publications

• Theytaz F, de Giorgi S, Hodson L, Stefanoni N, Rey V, Schneiter P, Giusti V, Tappy L. Metabolic fate of fructose ingested with and without glucose in a mixed meal. Nutrients. 2014 Jul 15;6(7):2632-49. doi: 10.3390/nu6072632.

Study record dates

Study Major Dates

• Study Start: February 1, 2013
• Primary Completion (ACTUAL): December 1, 2016
• Study Completion (ACTUAL): December 1, 2016

Study Registration Dates

• First Submitted: February 12, 2013
• First Submitted That Met QC Criteria: February 13, 2013
• First Posted (ESTIMATE): February 15, 2013

Study Record Updates

• Last Update Posted (ACTUAL): March 25, 2019
• Last Update Submitted That Met QC Criteria: March 22, 2019
• Last Verified: March 1, 2019

More Information

Terms related to this study

• Keywords: fructose; de novo lipogenesis; roux-en-Y gastric bypass; post-prandial triglycerides
• Other Study ID Numbers: 193/11